Immunostimulatory mouse granuloma protein.

Earlier studies have shown that from subcutaneous talc-induced granuloma in mice, a fraction could be extracted that fully protected mice against Listeria monocytogenes. Using standard biochemical procedures--i.e., ammonium sulfate fractionation, preparative electrophoresis, gel filtration chromatography, isoelectric focusing, and preparative polyacrylamide gel electrophoresis--we have now purified an active factor to homogeneity. A single band was obtained in NaDodSO4/polyacrylamide gel with an apparent Mr of 55,000. It migrated with alpha 1-globulins and the isoelectric point was 5 +/- 0.1. The biological activity was destroyed with Pronase but not with trypsin and a monospecific polyclonal rabbit antiserum was obtained. The intravenous injection of 5 micrograms of this "mouse granuloma protein" fully protects mice against a lethal inoculum of L. monocytogenes. Moreover, after their incubation with 10 nM mouse granuloma protein, mouse peritoneal cells became cytostatic against Lewis carcinoma cells

Antiinflammatory effects of murine malignant cells.

Development of teratocarcinoma does not impair immunization of mice against Listeria monocytogenes. Endotoxin injection a short time before tumor cell inoculation allows the growth of teratocarcinoma in non syngenic mice despite immune stimulation. In contrast with this absence of impaired systematic immunity, teratocarcinoma cells were found to repulse macrophages in vitro. This effect on macrophages was also found with three other malignant cells and with trophoblast cells. In vivo, teratocarcinoma cells were found to impair local inflammation. These cells and other malignant cells are able to produce a compound(s) of molecular weight between 10(3) and 10(4), which prevents inflammatory reaction. These results suggest that mouse teratocarcinomas and other tumors by-pass the host immunological system of surveillance by at least two mechanisms: a direct toxic effect on macrophages and the release of an inhibitor of inflammation. The possible relations between these properties of malignant cells and physiological functions of trophoblast are discussed

Attitudes et couverture vaccinale antigrippal des soignants d'un établissement de santé universitaire

International audienc

Automated Surface Wave Measurements for Evaluating the Depth of Surface-Breaking Cracks in Concrete

The primary objective of this study is to investigate the feasibility of an innovative surface-mount sensor, made of a piezoelectric disc (PZT sensor), as a consistent source for surface wave velocity and transmission measurements in concrete structures. To this end, one concrete slab with lateral dimensions of 1500 by 1500 mm and a thickness of 200 mm was prepared in the laboratory. The concrete slab had a notch-type, surface-breaking crack at its center, with depths increasing from 0 to 100 mm at stepwise intervals of 10 mm. A PZT sensor was attached to the concrete surface and used to generate incident surface waves for surface wave measurements. Two accelerometers were used to measure the surface waves. Signals generated by the PZT sensors show a broad bandwidth with a center frequency around 40 kHz, and very good signal consistency in the frequency range from 0 to 100 kHz. Furthermore, repeatability of the surface wave velocity and transmission measurements is significantly improved compared to that obtained using manual impact sources. In addition, the PZT sensors are demonstrated to be effective for monitoring an actual surface-breaking crack in a concrete beam specimen subjected to various external loadings (compressive and flexural loading with stepwise increases). The findings in this study demonstrate that the surface mount sensor has great potential as a consistent source for surface wave velocity and transmission measurements for automated health monitoring of concrete structures