RNAseq analysis of mutants in coding and non-coding transcription of phosphate genes in the yeast Saccharomyces cerevisiae

Microbial BiotechnologyPlant science

Transcriptional response of Saccharomyces cerevisiae to potassium starvation

Plant science

Visualization of VirE2 protein translocation by the Agrobacterium type IV secretion system into host cells

Plant science

Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4

Plant science

Image analysis platform for yeast biologists

Plant science

Graphical analysis and experimental evaluation of Saccharomyces cerevisiae P_TRK1|2 and P_BMH1|2 promoter region

Plant sciencesMicrobial Biotechnolog

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic

Self-association of the spindle pole body-related intermediate filament protein Fin 1p and its phosphorylation-dependent interaction with 14-3-3 proteins in yeast

Moleculaire ontwikkelingsgenetic

Cell-to-cell heterogeneity of phosphate gene expression in yeast is controlled by alternative transcription, 14-3-3 and Spl2

Dependent on phosphate availability the yeast Saccharomyces cerevisiae expresses either low or high affinity phosphate transporters. In the presence of phosphate yeast cells still express low levels of the high affinity phosphate transporter Pho84. The regulator Spl2 is expressed in approximately 90% of the cells, and is not expressed in the remaining cells. Here we report that deletion of RRP6, encoding an exonuclease degrading non-coding RNA, or BMH1, encoding the major 14-3-3 isoform, resulted in less cells expressing SPL2 and in increased levels of RNA transcribed from sequences upstream of the SPL2 coding region. SPL2 stimulates its own expression and that of PHO84 ensuing a positive feedback. Upon deletion of the region responsible for upstream SPL2 transcription almost all cells express SPL2. These results indicate that the cell-to-cell variation in PHO84 and SPL2 expression is dependent on a specific part of the SPL2 promoter and is controlled by Bmh1 and Spl2.Microbial Biotechnolog

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic