Pengelompokan Klon-Klon Ubi Jalar Berdasarkan Analisis Gerombol, Komponen Utama dan Biplot dari Karakter Morfologi

Sweet potato is a food crop with high genetic and phenotypic diversities. The objective of this study was to cluster sweet potato clones based on their morphological characters. This research used descriptive method using 25 morphological characters on 28 sweet potato clones and clustered using dice coefficient, principal component analysis and biplot analysis. The results showed that the clones were grouped into two clusters and 14 sub-clusters. Three sub-clusters had high similarity coefficients, i.e., 0.68-0.96, one clone was eliminated, and 11 sub-clusters had a low similarity, i.e., 0.41-0.52. The principal component analysis showed 14 of 25 morphological characters determined the diversity in 27 sweet potato clones with cumulative variance of 70.79%. Biplot analysis showed that 12 characters contributed to cumulative variance of 61.3%. Twelve morphological characters had weak contribution on the characteristics of the clones in quadrant I; meanwhile, characteristics of clones in quadrant II were orange, yellow and white tuber flesh, in quadrant III was dark purple tuber flesh, and in quadrant IV were purple tuber flesh and cortex. Result of the grouping analysis identified clones that were closely related and those distantly related for improvement purposes

Keragaan Pertumbuhan dan Produksi Beberapa Aksesi Kacang Tunggak (Vigna Unguiculata (L) Walp) di Desa Watidal Kabupaten Maluku Tenggara Barat

Cowpea (Vigna unguiculata (L) Walp) of the District of West Southeast Maluku (MTB) needs to be evaluated in the region of its origin. The objectives this research was to study the growth patterns of cowpea, to obtain local cowpea accessions with agronomic traits as expected, as well as high production potential. This research was conducted at Watidal village, MTB District, Maluku using a randomized completely block design (RCBD) with three replications. Factors to be tested was 15 accessions of cowpea. The observed variables were some vegetative characters, yield component and yield. Measured data were analyzed of its variance, and if the effects of treatments were highly significant then it is followed by Duncan difference test. The results showed differences in plant growth patterns, number of leaves, and number of internodes between accessions at 8 weeks after planting, and apparent at week 16. At week 16, most accessions showed a slowdown in growth rates including improved varieties (KT6). The result of variance test shows that the variation of plant height is significantly different, so that the variables of leaves number of branches, and all different variables are very real. Duncan\u27s different test results show the appearance of lush growth through the number of leaves, the number of books and the number of branches more in accession KTm7, KTm27, KTm9, and KTm4, while some other local accessions including superior varieties showed a slight growth of leaf number. The results showed six accessions of KTm24, KTm13, KTm14, KTm12, KTm9, and KTm5 had the highest seed weight of the crop. Three accessions of local cowpea KTm5, KTm12 and KTm9 had the highest production components by weight of pod (g) and number of first pods

Akurasi Karakterisasi Tingkat In situ Tanaman Ubi Jalar pada Dua Kecamatan di Kabupaten Seram Bagian Barat

Karakter morfologi tajuk ubi jalar dapat digunakan sebagai pembeda antar aksesi. Tujuan penelitian untuk mendapatkan sejumlah klon ubi jalar yang beragam berdasarkan morfologi tajuk serta ketepatan karakterisasi morfologi pada tingkat in situ. Penelitian ini menggunakan metode survei di lima desa pada dua kecamatan yaitu kecamatan Inomosol dan Huamual Muka pada Kabupaten Seram Bagian Barat, Propinsi Maluku. Tahap pertama dengan mengkarakterisasi morfologi tajuk di kebun petani (in situ), dan tahap kedua penanaman dan karakterisasi ex situ di kebun koleksi pada umur tanaman 2 bulan menggunakan deskriptor ubi jalar. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa terdapat 25 aksesi ubi jalar di kecamatan Inomosol dan Huamual Muka yang dibedakan berdasarkan bentuk dan warna daun, pola dan jumlah lekuk daun, warna tangkai dan batang, serta sifat membelit. Hasil verifikasi terhadap karakterisasi in situ didapatkan akurasi ≥80% pada 3 fenotip pada pola lekuk daun, daun dengan bentuk bagian tengah linear, daun dengan jumlah cuping 1, 5 dan 7, tulang daun permukaan bawah berwarna hijau, 2 fenotip pada warna tangkai daun, batang berwarna merah keunguan, serta ada warna tambahan pada batang. Sedangkan akurasi karakterisasi pada fenotip lainnya yaitu 0-76%. Kata kunci: ex situ, Ipomoea batatas, keragaman, Maluku, morfolog

AKURASI KARAKTERISASI TINGKAT IN SITU TANAMAN UBI JALAR PADA DUA KECAMATAN DI KABUPATEN SERAM BAGIAN BARAT

Morphological characters of above ground plant parts can be used to distinguishsweet potato accessions. The objective of this study was to get a number of diversity of sweet potato accessions based on above ground part morphology and to determine the accuracy of in situ characterization. This study used a survey method in five villages in two sub-districts, namely Inomosol and Huamual Muka, Western Ceram District. The first stage of this study involved in situ characterization, and the second stage involved planting and ex situ morphological characterization of 2-month-old plants which was used sweet potato descriptors. Data were analyzed descriptively and qualitatively. The results showed that there were 25 accessions of sweet potato found in Huamual Muka and Inomosol Sub-districts, as distinguished by leaf shape and color, leaf patterns and leaf lobe number, petiole and stem colors, and vine twisting tendency. Verification results of the above ground morphology showed accuracy of ≥80% in 3 phenotypes in leaf lobe pattern, shape of central leaf lobe, linear middle leaves, leaves with 1, 5 and 7 lobes, green abaxial leaf veins, 2 petiole color phenotypes, purplish red stem, and stem additional colors. Meanwhile, the accuracy of characterization of other phenotypes was 0-76%. Keywords: diversity, ex situ, Ipomoea batatas, Maluku, morphology   ABSTRAK Karakter morfologi tajuk ubi jalar dapat digunakan sebagai pembeda antar aksesi. Tujuan penelitian untuk mendapatkan sejumlah klon ubi jalar yang beragam berdasarkan morfologi tajuk serta ketepatan karakterisasi morfologi pada tingkat in situ. Penelitian ini menggunakan metode survei di lima desa pada dua kecamatan yaitu kecamatan Inomosol dan Huamual Muka pada Kabupaten Seram Bagian Barat, Propinsi Maluku. Tahap pertama dengan mengkarakterisasi morfologi tajuk di kebun petani (in situ), dan tahap kedua penanaman dan karakterisasi ex situ di kebun koleksi pada umur tanaman 2 bulan menggunakan deskriptor ubi jalar. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa terdapat 25 aksesi ubi jalar di kecamatan Inomosol dan Huamual Muka yang dibedakan berdasarkan bentuk dan warna daun, pola dan jumlah lekuk daun, warna tangkai dan batang, serta sifat membelit. Hasil verifikasi terhadap karakterisasi in situ didapatkan akurasi ≥80% pada 3 fenotip pada pola lekuk daun, daun dengan bentuk bagian tengah linear, daun dengan jumlah cuping 1, 5 dan 7, tulang daun permukaan bawah berwarna hijau, 2 fenotip pada warna tangkai daun, batang berwarna merah keunguan, serta ada warna tambahan pada batang. Sedangkan akurasi karakterisasi pada fenotip lainnya yaitu 0-76%. Kata kunci: ex situ, Ipomoea batatas, keragaman, Maluku, morfolog

Karakterisasi Morfologi Bunga dan Buah Abnormal Kelapa Sawit (Elaeis Guineensis Jacq) Hasil Kultur Jaringan

Tissue culture-derived plants of oil palm (Elaeis guineensis Jacq) can produce abnormal female dan male flowers, especially female, which develop into mantled fruit. This abnormality have caused yield lost up to 40%. The objectives of this research were to study the stages of flower development in which the abnormality started to appear, to characterize the morphology of the abnormal flower and fruit, and to determine the degree of abnormality. Plant materials used in this research were MK152, MK 176, MK 203, MK163, MK104, MK 212 and MK 209 clones obtained from the collection of "Badan Pengkajian dan Penerapan Teknologi (BPPT)" in Ciampea, Bogor. Characterization of abnormality at the flowering stage was conducted based on morphological performance at several stages of flower development, while the degree of abnormality at fruiting stage was determined based on the condition of carpel, mesocarp and seed. Normal flower and fruit of the same clone were used as the control. The results showed that the abnormality started at primordial stage of flower organ formation. Supplement carpel was found surrounding gynoecium for the abnormal female flower, which was composed of three to six carpels present at separate whorl of flower. Abnormal male flower had no additional whorl of flower, instead stamens were converted into carpel-like structures. In this case, number of carpels structure depends on the degree of abnormality. There were three levels of abnormality, i.e. light abnormality (AbR), heavy abnormality (AbB) and severe abnormality (AbSB). The severe abnormal fruit was seedless, with soft mesocarp (AbSB1) and woody mesocarp (AbSB2)

Keragaman dan Potensi Genetik Ubi Jalar Lokal Pada Kondisi Partial Submergence

Excess ground water above normal due to high rainfall affect the growth and yields of crops. The objectives of the study were to measure the genetic diversity of local sweet potato clones, to obtain characters of vegetative and yield characters that were  responsive against of partial submergence and sweet potato clones that had highest yield components. The method of research was a one-factor experiment involving 21 sweet potato clones using a Completely Randomized Block design with 3 replication. The partial submergence in this study mean that was the lower parts of the plants submerged when the plant were 2 and 3 months old. Data were analyzed with F test, genotype and phenotype variance, and DMRT test. The results show the sweet potato clones have a wide diversities of vegetative and yield characters. Vegetative characters that were positively responsive to partial submergence were the number of green leaves, leaf area per plant, leaf area index, and stem length. Yield characters that were positively responsive to partial submergence were tuber number and tuber length. Some of the clones with the highest production potential were shown through two of the three yields indicators (tubers number, individual tuber weight and tuber weight per plant), i.e. A13, AR15, B9, BSepa-U, BR5, H1 clones and Antin 2 variety.. Keywords: excess water, genotype variance, Ipomoea batatas, Maluku, yield character   ABSTRAK Kelebihan air tanah di atas normal akibat curah hujan yang tinggi mempengaruhi pertumbuhan dan produksi tanaman. Tujuan penelitian untuk mengukur keragaman genetik klon-klon ubi jalar lokal,  serta mendapatkan karakter vegetatif dan karakter hasil yang responsif positif terhadap partial submergence dan klon ubi jalar yang mempunyai komponen hasil tertinggi. Metode penelitian berupa percobaan satu faktor yaitu 21 klon ubi jalar menggunakan rancangan acak lengkap berblok dengan pengulangan tiga kali. Partial submergence  dalam penelitian ini yaitu  bagian bawah dari tanaman terendam air pada umur tanaman 2 dan 3 bulan. Analisis data dengan uji F, ragam genotip dan fenotip, dan uji DMRT. Hasil menunjukkan bahwa klon-klon ubi jalar mempunyai  keragaman luas pada semua karakter vegetatif dan produksi. Karakter vegetatif  yang responsif positif terhadap  partial submergence yaitu jumlah daun hijau, luas daun per tanaman, indeks luas daun dan panjang batang. Karakter produksi yang responsif positif terhadap  partial submergence yaitu jumlah umbi dan panjang umbi. Beberapa klon dengan potensi produksi melalui dua dari tiga indikator produksi (jumlah umbi, bobot per umbi dan bobot umbi segar per tanaman) tertinggi yaitu  A13, A15, B9,  BSepa-U, BR5, H1, dan varietas Antin 2. Kata kunci: Ipomoea batatas, karakter hasil, Maluku, ragam genotip, terenda