Mortality, SAH grading, body weight, and neurological score.

<p>A and B: Mortality and SAH grading, no significant difference between the SAH+vehicle group and the SAH+BBG group. C: SAH induced body weight loss, BBG administration increased body weight without a significant difference. D: BBG administration improved neurological deficits at 24 hours after SAH. B and C, Error bars represent mean ± standard error of the mean. D, Error bars represent median ±25th–75th interquartile percentiles. * <i>p</i><0.05 vs. Sham; ** <i>p</i><0.01 vs. Sham; # <i>p</i><0.05 vs. SAH+vehicle group.</p

Quantitative analysis of mature IL-1β(A), MPO (B), Occludin (C), and ZO-1 (D) expressions 24 hours post-SAH.

<p>1, sham group; 2, SAH+Vehicle group; 3, SAH+BBG group. n = 6 rats per group. Error bars represent mean ± standard error of the mean. * <i>p</i><0.05 vs. Sham; ** <i>p</i><0.01 vs. Sham; # <i>p</i><0.05 vs. SAH+vehicle group. Correlation between mature IL-1β and neurobehavioral score (E).</p

Change in the P-index at 24 hours after SAH.

<p>A: The P-index of NPE was significantly higher than non-NPE in SAH rats. @ <i>p</i><0.05 vs. NPE; B: BBG decreased the incidence of NPE without reaching significance; C: BBG significantly reduced the P-index at 24 hours after SAH. Error bars represent mean ± standard error of the mean. * <i>p</i><0.05 vs. Sham; ** <i>p</i><0.01 vs. Sham; # <i>p</i><0.05 vs. SAH+vehicle group.</p

Lung histopathology at 24 hours after SAH.

<p>(A) Zymography assay for MMP-9 activity in the left lung in sham, vehicle, and BBG treated groups. (B) Representative microphotographs of immunofluorescence staining showing co-localization of T1-α (FITC/green), P2X7R (Texas red/red) and DAPI (blue). Scale bar = 100 µm. (C) H&E staining showed BBG administration ameliorated diffuse neutrophilic infiltration and swelling of the alveolar interstitium.</p