33 research outputs found

    Draft genome assembly of two <i>Pseudoclavibacter helvolus</i> strains, G8 and W3, isolated from slaughterhouse environments

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    We report the draft genome sequences of two Pseudoclavibacter helvolus strains. Strain G8 was isolated from a meat chopper and strain W3 isolated from the wall of a small slaughterhouse in Denmark. The two annotated genomes are 3.91 Mb and 4.00 Mb in size, respectively

    Genome sequence of <i>Arthrobacter antarcticus</i> strain W2, isolated from a slaughterhouse

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    We report the draft genome sequence of Arthrobacter antarcticus strain W2, which was isolated from a wall of a small slaughterhouse in Denmark. The 4.43-Mb genome sequence was assembled into 170 contigs

    Genome sequence of <i>Kocuria palustris</i> strain W4

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    We report the 3.09 Mb draft genome sequence of Kocuria palustris W4, isolated from a slaughterhouse in Denmark

    Genome sequence of <i>Kocuria varians</i> G6 ssolated from a slaughterhouse in Denmark

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    We report here the first draft genome sequence of Kocuria varians G6, which was isolated from a meat chopper at a small slaughterhouse in Denmark. The 2.90-Mb genome sequence consists of 95 contigs and contains 2,518 predicted protein-coding genes

    Draft genome sequences of two <i>Kocuria</i> isolates, K. <i>salsicia</i> G1 and K. <i>rhizophila</i> G2, isolated from a slaughterhouse in Denmark

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    We report here the draft genome sequences of Kocuria salsicia G1 and Kocuria rhizophila G2, which were isolated from a meat chopper at a small slaughterhouse in Denmark. The two annotated genomes are 2.99 Mb and 2.88 Mb in size, respectively

    Genome sequence of <i>Psychrobacter cibarius</i> strain W1

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    Here, we report the draft genome sequence of Psychrobacter cibarius strain W1, which was isolated at a slaughterhouse in Denmark. The 3.63-Mb genome sequence was assembled into 241 contigs

    A meta-proteomics approach to study the interspecies interactions affecting microbial biofilm development in a model community

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    Abstract Microbial biofilms are omnipresent in nature and relevant to a broad spectrum of industries ranging from bioremediation and food production to biomedical applications. To date little is understood about how multi-species biofilm communities develop and function on a molecular level, due to the complexity of these biological systems. Here we apply a meta-proteomics approach to investigate the mechanisms influencing biofilm formation in a model consortium of four bacterial soil isolates; Stenotrophomonas rhizophila, Xanthomonas retroflexus, Microbacterium oxydans and Paenibacillus amylolyticus. Protein abundances in community and single species biofilms were compared to describe occurring inter-species interactions and the resulting changes in active metabolic pathways. To obtain full taxonomic resolution between closely related species and empower correct protein quantification, we developed a novel pipeline for generating reduced reference proteomes for spectral database searches. Meta-proteomics profiling indicated that community development is dependent on cooperative interactions between community members facilitating cross-feeding on specific amino acids. Opposite regulation patterns of fermentation and nitrogen pathways in Paenibacillus amylolyticus and Xanthomonas retroflexus may, however, indicate that competition for limited resources also affects community development. Overall our results demonstrate the multitude of pathways involved in biofilm formation in mixed communities

    Unravelling the mechanisms of bacterial interactions in model communities

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