77 research outputs found

    The role of the gut microbiota in the dietary niche expansion of fishing bats

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    This is the final version. Available from BMC via the DOI in this record. Availability of data and materials: The raw data have been deposited at the NCBI SRA database under the project accession number PRJEB47836. Bioinformatics pipelines used to process the sequence data and generate count tables are available at https://github. com/ostaizka/fishing- bats.Background: Due to its central role in animal nutrition, the gut microbiota is likely a relevant factor shaping dietary niche shifts. We analysed both the impact and contribution of the gut microbiota to the dietary niche expansion of the only four bat species that have incorporated fish into their primarily arthropodophage diet. Results: We first compared the taxonomic and functional features of the gut microbiota of the four piscivorous bats to that of 11 strictly arthropodophagous species using 16S rRNA targeted amplicon sequencing. Second, we increased the resolution of our analyses for one of the piscivorous bat species, namely Myotis capaccinii, and analysed multiple populations combining targeted approaches with shotgun sequencing. To better understand the origin of gut microorganisms, we also analysed the gut microbiota of their fish prey (Gambusia holbrooki). Our analyses showed that piscivorous bats carry a characteristic gut microbiota that differs from that of their strict arthropodophagous counterparts, in which the most relevant bacteria have been directly acquired from their fish prey. This characteristic microbiota exhibits enrichment of genes involved in vitamin biosynthesis, as well as complex carbohydrate and lipid metabolism, likely providing their hosts with an enhanced capacity to metabolise the glycosphingolipids and longchain fatty acids that are particularly abundant in fish. Conclusions: Our results depict the gut microbiota as a relevant element in facilitating the dietary transition from arthropodophagy to piscivory.The Carlsberg Foundation’s Distinguished Postdoctoral FellowshipDanish National Research FoundationVillum Fonden GrantLundbeckfonden GrantDanish Council for Independent Research GrantConsejo Nacional de Ciencia y Tecnologí

    Search for invisible decays of the Higgs boson produced via vector boson fusion in proton-proton collisions at root s=13 TeV

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