16 research outputs found
Cell death profile after treatment with IC<sub>50</sub> of the ExEP-P and ExEP-A.
<p>(A) Dot plots indicating the flow cytometry, and (B) representative diagrams obtained via flow cytometry of cells stained with annexin V-FITC/PI; Anx<sup>–</sup>/PI<sup>–</sup>: viable cells; Anx<sup>+</sup>/PI<sup>–</sup>: apoptotic cells; Anx<sup>–</sup>/PI<sup>+</sup>: necrotic cells, and Anx<sup>+</sup>/PI<sup>+</sup>: cells in late apoptosis. ***p < 0.001 treated group versus control viable cells. <sup>###</sup>p < 0.001 treated group versus control apoptosis. <sup>+++</sup>p < 0.001 treated group versus control necrosis. <sup>xxx</sup>p < 0.001 and <sup>x</sup>p < 0.05 treated group versus control late apoptosis.</p
Antioxidant and cytotoxic activity of propolis of <i>Plebeia droryana</i> and <i>Apis mellifera</i> (Hymenoptera, Apidae) from the Brazilian Cerrado biome - Fig 3
<p>Cytotoxic activity of ExEP from (A) <i>P</i>. <i>droryana</i> and (B) <i>A</i>. <i>mellifera</i> against the peripheral blood mononuclear cells (PBMC) and K562 erythroleukemia cell lines.</p
Viability of K562 cells at 0, 4, 8, 24 and 48 h after treatment with IC<sub>50</sub> of the ExEP-P or ExEP-A.
<p>Images are representative of those seen from at least three such fields of view per sample and three independent repeats.</p
Effect of a necrosis inhibitor necrostatin-1 (NEC-1) on cell death mediated by ExEP-P and ExEP-A.
<p>*** p<0.0001 compared with the control group. # p<0.05 NEC-1+ ExEP-P compared with the ExEP-P.</p
DPPH free radical-scavenging activity (%) of ExEP at different concentrations (μg/mL).
<p>DPPH free radical-scavenging activity (%) of ExEP at different concentrations (μg/mL).</p
Compounds identified in nonpolar fraction of ExEP from <i>P</i>. <i>droryana</i> (ExEP-P) and <i>A</i>. <i>mellifera</i> (ExEP-A) by gas chromatography−mass spectrometry (GC-MS).
<p>Compounds identified in nonpolar fraction of ExEP from <i>P</i>. <i>droryana</i> (ExEP-P) and <i>A</i>. <i>mellifera</i> (ExEP-A) by gas chromatography−mass spectrometry (GC-MS).</p
Compounds identified in polar fraction of ExEP from <i>Plebeia droryana</i> (ExEP-P) and <i>Apis mellifera</i> (ExEP-A) by high-performance liquid chromatography (HPLC).
<p>Compounds identified in polar fraction of ExEP from <i>Plebeia droryana</i> (ExEP-P) and <i>Apis mellifera</i> (ExEP-A) by high-performance liquid chromatography (HPLC).</p
Image_5_First generation of multifunctional peptides derived from latarcin-3a from Lachesana tarabaevi spider toxin.TIF
The need for discovering new compounds that can act selectively on pathogens is becoming increasingly evident, given the number of deaths worldwide due to bacterial infections or tumor cells. New multifunctional biotechnological tools are being sought, including compounds present in spider venoms, which have high biotechnological potential. The present work aims to perform the rational design and functional evaluation of synthetic peptides derived from Lachesana tarabaevi spider toxin, known as latarcin-3a. The antimicrobial activity was tested against Gram-positive and -negative bacteria, with minimum inhibitory concentrations (MIC) between 4 and 128 μg.ml−1. Anti-biofilm tests were then performed to obtain MICs, where the peptides demonstrated activity from 4 to 128 μg.ml−1. In vitro cell cytotoxicity assays were carried out from tumor cell lines, lineages C1498, Kasumi-1, K-562, Jurkat, MOLT4, and Raji. Erythrocyte integrity was evaluated in the presence of synthetic peptides analog, which did not promote hemolysis at 128 μg.ml−1. The peptide that showed the best antibacterial activity was Lt-MAP3 and the best antitumor was Lt-MAP2. In conclusion, rational design of multifunctional antimicrobial peptides may be promising alternative tools in the treatment of emerging diseases such as bacterial infections and tumor cells.</p
Image_3_First generation of multifunctional peptides derived from latarcin-3a from Lachesana tarabaevi spider toxin.TIF
The need for discovering new compounds that can act selectively on pathogens is becoming increasingly evident, given the number of deaths worldwide due to bacterial infections or tumor cells. New multifunctional biotechnological tools are being sought, including compounds present in spider venoms, which have high biotechnological potential. The present work aims to perform the rational design and functional evaluation of synthetic peptides derived from Lachesana tarabaevi spider toxin, known as latarcin-3a. The antimicrobial activity was tested against Gram-positive and -negative bacteria, with minimum inhibitory concentrations (MIC) between 4 and 128 μg.ml−1. Anti-biofilm tests were then performed to obtain MICs, where the peptides demonstrated activity from 4 to 128 μg.ml−1. In vitro cell cytotoxicity assays were carried out from tumor cell lines, lineages C1498, Kasumi-1, K-562, Jurkat, MOLT4, and Raji. Erythrocyte integrity was evaluated in the presence of synthetic peptides analog, which did not promote hemolysis at 128 μg.ml−1. The peptide that showed the best antibacterial activity was Lt-MAP3 and the best antitumor was Lt-MAP2. In conclusion, rational design of multifunctional antimicrobial peptides may be promising alternative tools in the treatment of emerging diseases such as bacterial infections and tumor cells.</p
Image_2_First generation of multifunctional peptides derived from latarcin-3a from Lachesana tarabaevi spider toxin.TIF
The need for discovering new compounds that can act selectively on pathogens is becoming increasingly evident, given the number of deaths worldwide due to bacterial infections or tumor cells. New multifunctional biotechnological tools are being sought, including compounds present in spider venoms, which have high biotechnological potential. The present work aims to perform the rational design and functional evaluation of synthetic peptides derived from Lachesana tarabaevi spider toxin, known as latarcin-3a. The antimicrobial activity was tested against Gram-positive and -negative bacteria, with minimum inhibitory concentrations (MIC) between 4 and 128 μg.ml−1. Anti-biofilm tests were then performed to obtain MICs, where the peptides demonstrated activity from 4 to 128 μg.ml−1. In vitro cell cytotoxicity assays were carried out from tumor cell lines, lineages C1498, Kasumi-1, K-562, Jurkat, MOLT4, and Raji. Erythrocyte integrity was evaluated in the presence of synthetic peptides analog, which did not promote hemolysis at 128 μg.ml−1. The peptide that showed the best antibacterial activity was Lt-MAP3 and the best antitumor was Lt-MAP2. In conclusion, rational design of multifunctional antimicrobial peptides may be promising alternative tools in the treatment of emerging diseases such as bacterial infections and tumor cells.</p