Chemical constituents and antileukemic activity of <i>Eugenia dysenterica</i>

<p>The study about <i>Eugenia dysenterica</i> led to the isolation of 3<i>-</i>acetyl<i>-</i>urs<i>-</i>12-en-28<i>-</i>oic (<b>1</b>), 3-acetyl-olean-12-en-28-oic acid (<b>2</b>) and isoquercetin (<b>3</b>) from the stem barks, and of 3-<i>O</i>-β-glucopyranosyl-β-sitosterol (<b>4</b>), methyl 3-hydroxy-4-methoxybenzoate (<b>5</b>), methyl 4-hydroxyphenyl propionate (<b>6</b>), <i>E</i>-methyl-4-hydroxycinnamate (<b>7</b>), quercetin-3-<i>O</i>-(6ꞌꞌ-<i>O</i>-galloyl)-β-d-glucopyranoside (<b>8</b>) and quercetin-3-<i>O</i>-β-d-galactopyranoside (<b>9</b>) from the leaves. The structures <b>1</b>–<b>9</b> were set through the analysis of their NMR spectroscopic data. Compounds <b>2</b>, <b>3</b> and <b>5</b>–<b>8</b> were reported for the first time in the <i>Eugenia</i> genus. Compound <b>8</b> reduced cell viability and presented IC₅₀ values 40.3 and 36.7 μM, for the CCRF-CEM and the Kasumi-1 cells, respectively.</p

[1-8-NαC]-Zanriorb A1, a Proapoptotic Orbitide from Leaves of <i>Zanthoxylum riedelianum</i>

A new orbitide named [1-8-NαC]-zanriorb A1 (<b>1</b>) was isolated and characterized from the leaves of <i>Zanthoxylum riedelianum</i> using NMR and mass spectrometry. The absolute configuration of the amino acids was determined using Marfey’s method on the acid hydrolysates. Compound <b>1</b> induced cell death by apoptosis in Jurkat leukemia T cells (IC₅₀ 218 nM)