Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC)

Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible,biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercialfi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing bloodcoagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partialthromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability toenhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachmentand proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells couldattach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cantdifferences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed thatiCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissueculture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration

PENGARUH APLIKASI SISTEM PERANCAH NON FREEZE-DRYING CHA-GELATIN SHEET PADA PROLIFERASI DAN EKSPRESI GEN DIFERENSIASI OSTEOGENIK Runx2, Osterix DAN Osteocalcin SEL PUNCA MESENKIM DARI TALI PUSAT BAYI

Bone has a limited ability to perform self-repair and tissue regeneration, thus becoming the world's health problems. Bone tissue engineering is found to be great solution of bone regeneration therapy. The three basic elements in bone tissue engineering are (1) progenitor cells, (2) extracellular matrix scaffold system as required for the initial proliferation, migration and differentiation of cells, and (3) growth factors. Carbonate Apatite (CHA)-gelatin sheet, as a scaffold system for bone tissue engineering, has been developed by Tissue Engineering team in Faculty of Dentistry, University of Gadjah Mada. The mesenchymal stem cells also known as progenitor cells that have osteoblast-lineage potential. Therefore, in order to design a bone implant that can be tolerated human body, it is need to be tested the effect of the application system based on a synthetic scaffold CHA-gelatin sheet on the ability of proliferation and osteogenic differentiation of mesenchymal stem cells. In this study, mesenchymal stem cells obtained from human umbilical cord because it is one of the richest source of mesenchymal stem cells, and can be isolated easily and non-invasive by explant method. CHA-gelatin sheet was fabricated with two variations of the concentration (solid dissolved in liquid), which are 10 % w/v (CHA/gel-10) and 5% w/v (CHA/gel-05). Each variant uses 25% CHA and 75 % gelatin ratio. Proliferation test performed using the MTT method to determine the ability of proliferation induced by CHA-gelatin scaffold sheet on mesenchymal stem cells. Furthermore, semi-quantitative analysis of gene expression of osteogenic markers Runx2 , Osterix and Osteocalcin using RT-PCR was performed to determine the ability of osteogenic differentiation induction system CHA-gelatin scaffold sheet on mesenchymal stem cells . The results of this study indicate CHA/gel-05 scaffold capable of inducing proliferation of mesenchymal stem cells during 7 days of observation time. Then on osteogenic differentiation observation, scaffolding CHA/gel-10 able to increase the expression of Osterix and Osteocalcin genes of mesenchymal stem cells on day 10 of incubation time. Thus CHA-gelatin sheet scaffold are known to have the ability to induce proliferation and increase osteogenic differentiation of mesenchymal stem cells from human umbilical cord . However, it still needs further in-vivo testin

Difference between Microscopic and PCR Examination Result for Malaria Diagnosis and Treatment Evaluation in Sumba Barat Daya, Indonesia

Microscopic examination is the backbone of malaria diagnosis and treatment evaluation in Indonesia. This test has limited ability to detect malaria at low parasite density. Inversely, nested polymerase chain reaction (PCR) can detect parasites at a density below the microscopic examination’s detection limit. The objective of this study is to compare microscopic and PCR results when being used to identify malaria in suspected patients and patients who underwent dihydroartemisinin–piperaquine (DHP) therapy in the last 3–8 weeks with or without symptoms in Sumba Barat Daya, Nusa Tenggara Timur, Indonesia. Recruitment was conducted between April 2019 and February 2020. Blood samples were then taken for microscopic and PCR examinations. Participants (n = 409) were divided into three groups: suspected malaria (42.5%), post-DHP therapy with fever (4.9%), and post-DHP therapy without fever (52.6%). Microscopic examination found five cases of P. falciparum + P. vivax infection, while PCR found 346 cases. All microscopic examinations turned negative in the post-DHP-therapy group. Conversely, PCR result from the same group yielded 29 negative results. Overall, our study showed that microscopic examination and PCR generated different results in detecting Plasmodium species, especially in patients with mixed infection and in patients who recently underwent DHP therapy