Healing incisional surgical wounds using Rose Hip oil in rats

Purpose: To evaluate incisional surgical wound healing in rats by using Rose Hip (Rosa rubiginosa L.) oil. Methods: Twenty-one days after the oophorectomy procedure, twenty-seven female, adult, Wistar rats were distributed into three groups: Control group (wound treatment with distilled water); Collagenase group (treatment with collagenase ointment); and Rose Hip group (wound treatment with Rose Hip oil). Each group was distributed according to the date of euthanasia: 7, 14 and 21 days. The wound was evaluated considering the macroscopic and microscopic parameters. Results: The results indicated differences in the healing of incisional wounds between treatments when compared to control group. Accelerated wound healing was observed in the group treated with Rose Hip oil in comparison to the control and collagenase, especially after the 14th day. Morphometric data confirmed the structural findings. Conclusion: There was significant effect in topical application of Rose Hip oil on incisional surgical wound healing

Effects of Veronica officinalis extract in the kidney of rats with induced sepsis

PURPOSE: To evaluate the effects of Veronica officinalis extract as a anti-inflammatory substance on the kidney function of rats subjected to cecal ligation and puncture, describing histopathological and biochemical findings. METHODS: Twenty rats (Rattus norvegicus) were distributed into four groups (N=5): Sham group (GS), normal standard animals; Sepsis group (GSep), submitted a cecal ligation and puncture (CLP); Veronica group (GV), administered only Veronica officinalis extract once daily by gavage from the first day until the seventh day, without sepsis induction; Sepsis-Veronica group (GSep-V), administered Veronica officinalis extract once daily by gavage from the first day of CLP until the seventh day procedure. After the euthanasia, blood was collected for assessment of kidney biochemical and histological analysis were performed. RESULTS: The statistical analysis showed that there was statistically significant in serum levels of creatinine between GS and GSep (p=0.0029); and GSep and GSep-V (p=0.0184). In addition, there was statistically significant of histopathological parameters observed between GS and GSep (p=0.0001); GSep and GV (p=0.0415); also between GSep and GSep-V (p=0.0003). CONCLUSION: Veronica officinalis extract promoted a significant anti-inflammatory effect on kidney function and reduction of tissue damage in rats with sepsis

Continuous versus interrupted suture technique in microvascular anastomosis in rats

<div><p>Abstract Purpose: To compare the continuous and interrupted suture technique on femoral artery on rats after vessel repair and 14 days after. Methods: Twenty rats were operated randomly divided into two group matched according to the suture technique used: interrupted or continuous. We performed a femoral anastomosis on the right femoral artery. We analyzed weight, arterial caliber, anastomosis time and patency after vessel repair and 14 days after. Results: There was no significant difference between groups in the weight (p=0.64), diameter of the femoral artery (p=0.95) and patency (p=1.00). The time spent in the anastomosis was 451 seconds in the continuous group and 718 seconds in the interrupted group, presenting significant difference (p<0.01). Conclusion: The continuous suture technique shows a similar patency rates than interrupted technique, however with a shorter time to perform the anastomosis.</p></div

Perconditioning associated to hypertonic saline solution on liver function improvement after ischemia/reperfusion injury

<div><p>Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. Methods: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. Results: Per+HSS group showed a lower degree of liver dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly improved liver function and reduced histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute liver injury induced by ischemia/reperfusion.</p></div