Vitamin A türevi içeren ışığa duyarlı lipozomlardan kontrollü doxorubicin salımı.

Drug delivery systems (DDS) have been an attractive approach to eliminate the drawbacks of conventional drug administration. Controlled and photoresponsive drug delivery systems have a special advantage; they deliver drugs more effectively. Liposomes are mostly preferred as drug carriers due to their ability to carry both hydrophilic and hydrophobic drugs, their being non-toxic and non-immunogenic. In this study, photoresponsive liposomes were prepared by incorporating vitamin A derivatives into the lipid bilayer of the liposomes for use in treatment of eye diseases that require frequent and continuous drug administration. Proliferative vitreoretinopathy was selected as a model disease and doxorubicin as the therapeutic agent. Doxorubicin loaded phosphatidyl choline (PC): cholesterol (CHOL): all-trans retinal (ATR) (7:1:3) liposomes were tested on RPE/D407 cells to determine the effect of UVA exposure (365 nm) on the release of doxorubicin, and therefore, cell viability. Among the different liposome compositions studied by exposing to UVA it was found that PC:CHOL:ATR (7:1:3) formulation responded the best. Antiproliferative effects of all-trans retinal (ATR) and doxorubicin were observedwhen tested on RPE/D407 cell line. Also, the interaction of the cells with doxorubicin and liposomes were studied with flow cytometry and confocal laser scanning microscopy (CLSM). It was found that liposomes or ATR can penetrate the cells while doxorubicin penetrates the nucleus.M.S. - Master of Scienc

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

IŞIĞA DUYARLI LİPOZOMLARDAN KONTROLLU İLAÇ SALIM

IŞIĞA DUYARLI LİPOZOMLARIN (DRVs) NEOVASKÜLER GÖZ HASTALIKLARININ TEDEVİSİ İÇİN İLAÇ TAŞIYICI SİSTEM OLARAK GELİŞTİRİLMESİ

IŞIĞA DUYARLI LİPOZOMLARIN (DRVs) NEOVASKÜLER GÖZ HASTALIKLARININ TEDEVİSİ İÇİN İLAÇ TAŞIYICI SİSTEM OLARAK GELİŞTİRİLMES

FEN BİLİMLERİ ENSTİTÜSÜ/LİSANSÜSTÜ TEZ PROJESİ

DOKU MÜHENDİSLİĞİ YÖNTEMİ LE HASTAYA ÖZEL ORTAPEDİK İMPLANT TASARIMI VE ÜRETİM

FLUORIDE-CONTAINING ANTI-CARIES pH-SENSITIVE RELEASE SYSTEM AND ITS EFFECT ON STREPTOCOCCUS MUTANS

The aim of this study was to evaluate the antimicrobial effects of a newly developed anti-caries pH-sensitive controlled release system against Streptococcus mutans. For this purpose 9, 30, and 45 mg of NaF were placed in multilayer polymeric structures consisting of three layers: poly-L-lactide (PLLA), 8% Eudragit E 100 + Eudragit RS 100, and Chitosan. The amount of fluoride ion (F) released was determined with an ionmeter. In order to assess the effect of this antimicrobial material releasing NaF in a controlled manner on S. mutans, the proliferation of this organism was recorded by preparing test discs with a standard surface area containing the active ingredient. In all the groups the F release began when the pH fell under 5.5, after 6 hr, and the number of S. mutans declined. In conclusion, the newly developed controlled release system can release intraorally the fluoride ion with its anti-caries properties when the pH falls below 5.5 and it may help significantly to improve caries prevention