1 research outputs found
Live Stimulated Raman Histology for the Near-Instant Assessment of Central Nervous System Samples
Central nervous system tumors encompass many heterogeneous
neoplasms
with different outcomes and treatment strategies. The current classification
of these tumors is based on molecular parameters in addition to histopathology
to define tumor entities. This genomic characterization of tumors
is also becoming increasingly essential for physicians to identify
targeted therapy options. The deployment of such genomic profiling
relies on an efficient surgical sampling. To perform an appropriate
tumor resection and a correct sampling of the tumor, the neurosurgeon
may request an intraoperative pathological consultation. Stimulated
Raman histology (SRH), an emerging nondestructive imaging technology,
can address this challenge. SRH allows for a rapid and label-free
microscopic examination of unprocessed tissues samples in near-perfect
concordance with standard histology. In this study we showed that
SRH enabled the near-instant microscopic examination of various central
nervous system samples without any tissue processing such as labeling,
freezing nor sectioning. Since SRH imaging is a nondestructive approach,
we demonstrated that the tissue could be readily recovered after SRH
imaging and reintroduced into the conventional pathology workflow
including immunohistochemistry and genomic profiling to establish
a definitive diagnosis