Immunoblot validation of protein markers in BAL.

<p>(A) Differential expression analysis based on DeCyder data for chosen protein markers. Every dot indicates the identified protein spot and its expression level in that particular study group. The difference in protein expression (average expression) between the groups is visualized with a continuous curve. Histone and semenogelin plots contains more than one curve due to several protein spot identification for histones H4, H2B (n = 3) and semenogelin I (n = 2) as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102624#pone.0102624.s005" target="_blank">Table S1</a>. (B) Images of immunoblot membranes and (C) immunoblot based validation results for protein markers. The number of samples in validation: CTR n = 19, AME n = 7–9, DBRI n = 17, HP n = 9, SARC n = 8. The means (black lines) are shown in scatter plots. *Indicates statistical significance, which is shown between experimental and control group, at the level of p≤0.05, **p≤0.01 and, ***p≤0.001.</p

Major symptoms and HRCT findings.

a<p>before BAL procedure.</p>b<p>HRCT = high-resolution computed tomography.</p>c<p>Hypersensitivity pneumonitis.</p

Immunoblot validation of protein markers in plasma.

<p>The immunoblot and validation analysis for (A) galectin-3 and (B<b>)</b> histone H2B from plasma samples. The number of samples in validation: CTR n = 16, HP n = 10, SARC n = 11. The means are shown in scatter plots. *Indicates statistical significance, which is shown between experimental and control group, at the level of p≤0.05, **p≤0.01 and, ***p≤0.001.</p

Patient characteristics.

<p>*Data available for ≥80% of samples.</p><p>**Data available for ≥75% of samples.</p>a<p>Damp building-related illness.</p>b<p>Agricultural type of microbial exposure.</p>c<p>Hypersensitivity pneumonitis.</p>d<p>Sarcoidosis.</p

The false color image of two-dimensional DIGE gel of BAL.

<p>The gel image represents the Cy3 labeled (red), Cy5 labeled (blue) and Cy2 labeled (yellow) patient samples. The latter is a pooled sample, which served as an internal standard. Spot abbreviations refer to the identified proteins listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0102624#pone.0102624.s005" target="_blank">Table S1</a>. Molecular weights are shown on the right edge of the gel and the pI range on the top part of the SDS-PAGE.</p