7 research outputs found
Influence of statin treatment on spontaneous cell migration.
<p>MPC (A) and MTT (B) were plated in vehicle (Ctr), 5 µM fluvastatin (Fluva), 5 µM simvastatin (Simva), 5 µM lovastatin (Lova) with or without 100 µM trans, trans farnesol (FOH) and spontaneous migration was recorded for 24.</p
Combination of two different statins to evaluate potential additive effects on MPC or MTT.
<p>Relative viability of MPC (A) and MTT (B) at the indicated doses and durations for the most effective combined treatments including simvastatin relative to simvastatin alone.</p
Effect of 6.25–50 µM statin treatment for 3 days on MPC and MTT proliferation.
<p>Percent significant results over all statins for all treatment doses at the different treatment durations for MPC (A) and MTT (B). The percentage of significant results was increased in MTT compared to MPC after 48 and 72 h, suggesting that MTT are more sensitive to statin treatment.</p
MAPK pathway representation in SDHB-derived PHEOs/PGLs.
<p>A. Western blot of pMAPK1/3, total MAPK1/3, and GAPDH in human PHEOs/PGLs. Patient and tumor information for each sample are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097712#pone-0097712-t001" target="_blank">Table 1</a>. Abbreviations: B) SDHB, D) SDHD, N) NF1, M) normal adrenal medulla. B. Heatmap showing expression of 21 MAPK pathway genes in pseudohypoxic PHEOs/PGLs. Expression of these 21 genes was significantly elevated in SDHB compared to normal medulla (p<0.002). Each sample was assigned a number. The corresponding sample identifier from the original article <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097712#pone.0097712-Shankavaram1" target="_blank">[33]</a> is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097712#pone.0097712.s001" target="_blank">Table S1</a>. Patient information and link to deposited data are given in the original article.</p
Relative proliferation of MPC and MTT under statin treatment.
<p>MPC (A–F) and MTT (G–K) were treated with ▪ 6.25 µM, ▴ 12.50 µM, ▾ 25.00 µM, and ⧫ 50.00 µM of atorvastatin, fluvastatin, lovastatin, pravastatin, rosuvastatin, and simvastatin for 24, 48, and 72 hours.</p
Expression of selected proteins in MPC and MTT after treatment with fluvastatin, simvastatin, or lovastatin.
<p>A. Western blot showing decreased levels of pMAPK1/3 in treated vs. untreated MPC and MTT relative to total MAPK1/3 and GAPDH. B. Western blot showing decreased levels of intact PARP (top bands) and increased levels of cleaved PARP (lower bands) in treated vs. untreated MPC and MTT. In accordance, cleaved CASP-3 was elevated in treated cells, indicating apoptosis. Cells were treated with 25 µM of the indicated statin for 48 hours.</p
Patient information.
<p>Abbreviations: A: adrenal, E: extra-adrenal, F: female, HN: head and neck, ID: identifier, M: male, MM: metastatic metastases, N: normal, P: primary non-metastatic, PM: primary metastatic.</p