The impacts of EMMPRIN glycosylation on the activation of the ERK/NF-κB signalling pathway.

<p>A representative Western blot illustrates the intercellular phosphorylation of ERK1/2 and IκBα when THP-1 cells were treated with HG-EMMPRIN (with or without the pre-treatment of PD98059), LG-EMMPRIN or the control medium. Quantitative analysis of 3 independent experiments is shown below, the band density was normalized to that of the total ERK1/2 and IκBα, respectively. * <i>P</i> < 0.05 compared with the control group (whose value was set as 1.0).</p

The impacts of EMMPRIN glycosylation on the expression and gelatinolytic activity of MMP-9 in THP1 cells.

<p>Representative Western blot (upper) and gelatine zymography (lower) results demonstrating the expression and the activity of MMP-9 by THP-1 cells in response to the treatment of HG-EMMPRIN (with or without pre-treatment of PD98059), LG-EMMPRIN or the control medium, respectively. Quantitative analysis of 3 independent experiments is shown on the right side. For Western blot, the band intensity was normalized to that of theβ-actin. *<i>P</i> < 0.05, compared with the control group (whose value was set as 1.0).</p

Changing of EMMPRIN glycosylation in response to pro-inflammatory stimuli in THP-1 cells.

<p>A representative Western blot demonstrates the changing of EMMPRIN glycosylation in response to PMA (A and B) or H₂O₂ (C and D) both in a concentration and time-dependent manner. Quantitative analysis of 3 independent experiments is shown for each treatment. The band intensity was normalized to that of theβ-actin. *<i>P</i> < 0.05, compared with the control group (whose value was set as 1.0).</p

The impact of EMMPRIN glycosylation on monocytes migration.

<p>A: Representative sections demonstrating migratory activities of THP-1 cells in response to the treatment of HG-EMMPRIN (with or without pre-treatment of PD98059), LG-EMMPRIN or the control medium, respectively, as measured by the Boyden chamber assay. B: Quantification of migratory cell numbers of 3 independent experiments. *<i>P</i> < 0.05, compared with the control group.</p

The impact of EMMPRIN glycosylation on monocytes-endothelium adhesion.

<p>A: Representative sections demonstrating the adhesive capabilities of fluorescently labelled THP-1 cells to the HUVEC monolayer when treated with HG-EMMPRIN (with or without pre-treatment of PD98059), LG-EMMPRIN or the control medium. B: Quantification of adhesive cell numbers of 3 independent experiments. *<i>P</i> < 0.05, compared with the control group.</p

Characterization of glycosylation patterns of EMMPRIN in THP-1 cells.

<p>A: A representative Western blot of 3 independent experiments reveals two differently glycosylated EMMPRIN proteins from THP-1 cell, which refer to HG-EMMPRIN (with larger molecular weight) and LG-EMMPRIN (with smaller molecular weight). The protein glycosylation had different response to the treatment of PNGase F or Endo H. B: A representative Western blot demonstrates that the expression of HG-EMMPRIN by THP-1 cells responsive to PMA induction was inhibited by swainsonine. C: Quantitative analysis of 3 independent experiments probing the change of HG-EMMPRIN (upper figure) and LG-EMMPRIN (lower figure) in response to PMA and swainsonine. The band intensity was normalized to that of the β-actin. *<i>P</i> < 0.05, compared with the control group (whose value was set as 1.0).</p

Echocardiographic follow-up at 90 days.

<p>Nearly half of the Grade-2 patients had LVEF recovered to normal (≥55%), while much fewer patients had improved LVEF in both Grade-3 and Grade-4 groups.</p

Rates of 90-day MACEs (defined as any death, resuscitated cardiac arrest, acute heart failure or stroke) were incremental with higher grades.

<p>Rates of 90-day MACEs (defined as any death, resuscitated cardiac arrest, acute heart failure or stroke) were incremental with higher grades.</p

The pathophysiological classification demonstrates comparable prognostic values as infarction size by C-statistics in the prediction of 90-day MACEs.

<p>The pathophysiological classification demonstrates comparable prognostic values as infarction size by C-statistics in the prediction of 90-day MACEs.</p

Logistic regression analysis for the predictor of 90-day MACEs.

<p>HTN: Hypertension; DM: Diabetes mellitus; LVEF: left ventricular ejection fraction</p><p>Logistic regression analysis for the predictor of 90-day MACEs.</p