Identifying Elements of \u3cem\u3eKinder- und Jugendliteratur\u3c/em\u3e

Kinder- und Jugendliteratur, children’s literature written for the purpose of teaching or entertaining young people, has been present in German literature since the Middle Ages. This genre has changed as German literature progressed, reflecting the developments of each era, including such periods as the Romantic, the Biedermeier, Realism, Modernism and Postmodernism. As such, we examined individual works of German Kinder- und Jugendliteratur for the purpose of identifying distinctive features which situate them within children’s literature as well as in the respective historical genre. The works examined were Nußknacker und Mausekönig (1816) by E. T. A. Hoffmann, Emil und die Detektive (1929) by Erich Kästner, Försters Pucki (1935) by Madge Trott, Jan und das Wildpferd (1957) by Heinrich Denneborg, Die Wolke (1987) by Gudrun Pausewang, and Tintenherz (2003) by Cornelia Funke. We present our findings in the form of a Wimmelbuch, a typical form of German Kinder- und Jugendliteratur made popular during the Biedermeier period of the mid-1800s

Pan-ethnic carrier screening and prenatal diagnosis for spinal muscular atrophy: clinical laboratory analysis of >72 400 specimens

Spinal muscular atrophy (SMA) is a leading inherited cause of infant death with a reported incidence of ∼1 in 10 000 live births and is second to cystic fibrosis as a common, life-shortening autosomal recessive disorder. The American College of Medical Genetics has recommended population carrier screening for SMA, regardless of race or ethnicity, to facilitate informed reproductive options, although other organizations have cited the need for additional large-scale studies before widespread implementation. We report our data from carrier testing (n=72 453) and prenatal diagnosis (n=121) for this condition. Our analysis of large-scale population carrier screening data (n=68 471) demonstrates the technical feasibility of high throughput testing and provides mutation carrier and allele frequencies at a level of accuracy afforded by large data sets. In our United States pan-ethnic population, the calculated a priori carrier frequency of SMA is 1/54 with a detection rate of 91.2%, and the pan-ethnic disease incidence is calculated to be 1/11 000. Carrier frequency and detection rates provided for six major ethnic groups in the United States range from 1/47 and 94.8% in the Caucasian population to 1/72 and 70.5% in the African American population, respectively. This collective experience can be utilized to facilitate accurate pre- and post-test counseling in the settings of carrier screening and prenatal diagnosis for SMA

Conscious uncoupling between FANCI and FANCD2 in DNA repair

The Fanconi anemia (FA)-BRCA pathway mediates repair of DNA interstrand crosslinks. The FA core complex, a multi-subunit ubiquitin ligase, participates in the detection of DNA lesions and monoubiquitinates two downstream FA proteins, FANCD2 and FANCI (or the ID complex). However, the regulation of the FA core complex itself is poorly understood. Here we show that the FA core complex proteins are recruited to sites of DNA damage and form nuclear foci in S and G2 phases of the cell cycle. ATR kinase activity, an intact FA core complex and FANCM-FAAP24 were crucial for this recruitment. Surprisingly, FANCI, but not its partner FANCD2, was needed for efficient FA core complex foci formation. Monoubiquitination or ATR-dependent phosphorylation of FANCI were not required for the FA core complex recruitment, but FANCI deubiquitination by USP1 was. Additionally, BRCA1 was required for efficient FA core complex foci formation. These findings indicate that FANCI functions upstream of FA core complex recruitment independently of FANCD2, and alter the current view of the FA-BRCA pathway

Optimal Design and Allocation of Electrified Vehicles and Dedicated Charging Infrastructure for Minimum Greenhouse Gas Emissions

Electrified vehicles, including plug-in hybrid electric vehicles (PHEVs) and battery electric vehicles (BEVs), have the potential to reduce greenhouse gas (GHG) emissions from personal transportation by shifting energy demand from gasoline to electricity. GHG reduction potential depends on vehicle design, adoption, driving and charging patterns, charging infrastructure, and electricity generation mix. We construct an optimization model to study these factors by determining optimal design of conventional vehicles (CVs), hybrid electric vehicles (HEVs), PHEVs, and BEVs and optimal allocation of vehicle designs and charging infrastructure in the fleet for minimum lifecycle GHG emissions over a range of scenarios. We focus on vehicles with similar size and acceleration to a Toyota Prius under urban EPA driving conditions. We find that under today’s U.S. average grid mix, the vehicle fleet allocated for minimum GHG emissions includes HEVs and PHEVs with ~30 miles (48 km) of electric range. Allocating only CVs, HEVs, PHEVs, or BEVs will produce 86%, 1%, 0%, or 13+% more life cycle GHG emissions, respectively. Unlike BEVs, PHEVs do consume some gasoline; however, PHEVs can power a large portion of vehicle miles on electrical energy while accommodating infrequent long trips without need for a large battery pack, with its corresponding production and weight implications. Availability of workplace charging for 90% of vehicles optimistically reduces optimized GHG emissions by 0.5%. Under decarbonized grid scenarios, larger battery packs are more competitive and reduce life cycle GHG emissions significantly. Future work will relax modeling assumptions and address life cycle cost and cost-effectiveness of GHG reductions.</p

Does Delay Length or Sequence Exposure Affect Repeated Acquisition Performance?

Color poster with text and graphs.Repeated acquisition of response chains is a systematic method used to study learning and memory. Subjects complete sequences of responses in order to earn reinforcers. Subjects must learn the correct sequence of responses for the session through trial-and-error. As the subject completes more correct sequences over session time, the number of errors should be reduced throughout the session. This within-session error reduction is defined as learning. The purpose of this study was to examine learning and memory by establishing baseline error rates for subjects and response sequences.University of Wisconsin--Eau Claire Office of Research and Sponsored Programs

Crystal structure and magnetic properties of the cluster complex tris(1-cysteinethiolato)pentacopper(2+) perchlorate monohydrate, a mixed-valence copper-mercaptide species

This article does not have an abstract

Vulnerability to (+)-Methamphetamine Effects and the Relationship to Drug Disposition in Pregnant Rats during Chronic Infusion

Chronic (+)-methamphetamine (METH) use during pregnancy increases the health risk for both mother and fetus. To provide insights into these risks, the relationship between changes in METH disposition and METH-induced pharmacological effects were studied in Sprague-Dawley rat dams and litters. Timed-pregnant rats (n = 5–6) were given saline or METH (5.6–17.8 mg/kg/day) by continuous sc infusion from gestational day (GD) 7 (before organogenesis) until GD21 (0–2 days before delivery). By GD11, all rats in the 17.8-mg/kg/day group died or were sacrificed for humane reasons. There were significant (p < 0.05) dose- and gestational time-dependent decreases in maternal body weight in the 10- to 13.2-mg/kg/day groups, which slowly recovered to near normal by GD21. Continued METH dosing in the surviving groups did not affect the mean pups/litter weight at the end of the experiment on GD21. While maternal and fetal METH and (+)-amphetamine serum concentrations were similar on GD21, brain concentrations were significantly greater in the dams (p < 0.05). Importantly, brain-to-serum ratios in the dams were 9:1 and 3:1 in the pups. METH systemic clearance (ClS) in dams significantly (p < 0.05) decreased from 52 ± 14 ml/min/kg on GD10 to 28 ± 6 ml/min/kg on GD21 in all dose groups, indicating late-gestational stage reductions in METH ClS. Overall, these findings suggest that there were two periods of increased susceptibility for dams and fetuses during chronic METH treatment. First was the period after the start of METH dosing in which neuroadaptation and tolerance to METH occurs in the adult. The second was at the end of pregnancy when METH clearance was significantly reduced