Supplementary Information - plos one publicatio

Full length western blot membrane imaging for IL6 (A) and alpha-tubulin (B) relating to cropped data presented in Fig.5 of Plos One paper. <br

Sgp130Fc transgenic mice exhibit a similar inflammatory phenotype in response to a MCD diet.

<p>Flow cytometry on livers from 4 weeks fed control and methionine and choline deficient diet (MCDD) mice was performed and showed (A) liver immune cells liver as percent of leukocytes (CD45+ cells) and (B) macrophages and Kuppfer cells inflammatory phenotype as percent of total macrophages and Kuppfer cells respectively. Data expressed as average ± SEM, n = 6–7. Statistical analysis by 2 way ANOVA, * p<0.05; ***p<0.001 Main effect for Control vs MCDD.</p

MCD diet induces NASH independently of IL-6 signalling.

<p>Liver samples from 4 weeks-fed control diet (CD) or methionine and choline deficient diet (MCDD) mice were homogenised and (A) mRNA expression of pro-inflammatory genes and macrophage marker was assessed by real time PCR and (B) protein expression of IL-6 and the loading control αTubulin was measured by Western blotting in the WT or transgenic animals. (C) Plasma and hepatic IL6 levels were measured in C57BL/6 fed a CD or MCDD for 4 weeks and injected with vehicle or sgp130Fc every second day. Data expressed as average ± SEM, n = 6–7. Statistical analysis by 2 way ANOVA, * p<0.05; **p<0.01; ***p<0.001 Main effect for Control vs MCDD; & p<0.05 for diet/genotype interaction.</p

SemAssist : assistance and assessment tools for semantic memory rehabilitation

International audienceSemantic memory, which is responsible for the acquisition and maintenance of knowledge, plays an essential role in most cognitive functions and thus in activities of daily living (ADL). One rehabilitation approach, with patients presenting semantic memory deficits, is to help them maintain their residual knowledge or relearn lost knowledge through the execution and repetition of ADLs. During a therapy session, a caregiver supervises and assists a patient performing a predefined ADL. But this rehabilitation process is difficult to put into practice outside therapy sessions and data are lacking to assess patient progress with respect to everyday routine. SemAssist is a cognitive assistance system implemented to fill this gap. On the one hand, SemAssist guides the patient through the steps of a predefined ADL at home, providing semantic information about objects involved in this ADL. SemAssist records interactions with the patient, providing ecological data on the rehabilitation proces

Sgp130Fc transgenic mice develop a similar NASH pathology to wild type mice on a MCD diet.

<p>Liver condition in 4 weeks fed control and methionine and choline deficient diet (MCDD) mice was assessed by studying (A) liver weight (expressed as % body weight), (B) liver enzymes ALT and AST, (C) liver histology with H&E staining and (D) intra-hepatic cholesterol concentrations. Data expressed as average ± SEM, n = 6–7. Statistical analysis by 2 way ANOVA, ***p<0.001 Main effect for Control vs MCDD; & p<0.05 for diet/genotype interaction.</p