9 research outputs found
Systems Insight into the Spore Germination of <i>Streptomyces coelicolor</i>
An example of bacterium, which undergoes a complex development,
is the genus of <i>Streptomyces</i> whose importance lies
in their wide capacity to produce secondary metabolites, including
antibiotics. In this work, a proteomic approach was applied to the
systems study of germination as a transition from dormancy to the
metabolically active stage. The protein expression levels were examined
throughout the germination time course, the kinetics of the accumulated
and newly synthesized proteins were clustered, and proteins detected
in each group were identified. Altogether, 104 2DE gel images at 13
time points, from dormant state until 5.5 h of growth, were analyzed.
The mass spectrometry identified proteins were separated into functional
groups and their potential roles during germination were further assessed.
The results showed that the full competence of spores to effectively
undergo active metabolism is derived from the sporulation step, which
facilitates the rapid initiation of global protein expression during
the first 10 min of cultivation. Within the first hour, the majority
of proteins were synthesized. From this stage, the full capability
of regulatory mechanisms to respond to environmental cues is presumed.
The obtained results might also provide a data source for further
investigations of the process of germination
Systems Insight into the Spore Germination of <i>Streptomyces coelicolor</i>
An example of bacterium, which undergoes a complex development,
is the genus of <i>Streptomyces</i> whose importance lies
in their wide capacity to produce secondary metabolites, including
antibiotics. In this work, a proteomic approach was applied to the
systems study of germination as a transition from dormancy to the
metabolically active stage. The protein expression levels were examined
throughout the germination time course, the kinetics of the accumulated
and newly synthesized proteins were clustered, and proteins detected
in each group were identified. Altogether, 104 2DE gel images at 13
time points, from dormant state until 5.5 h of growth, were analyzed.
The mass spectrometry identified proteins were separated into functional
groups and their potential roles during germination were further assessed.
The results showed that the full competence of spores to effectively
undergo active metabolism is derived from the sporulation step, which
facilitates the rapid initiation of global protein expression during
the first 10 min of cultivation. Within the first hour, the majority
of proteins were synthesized. From this stage, the full capability
of regulatory mechanisms to respond to environmental cues is presumed.
The obtained results might also provide a data source for further
investigations of the process of germination
Phlomis crinita
An example of bacterium, which undergoes a complex development,
is the genus of <i>Streptomyces</i> whose importance lies
in their wide capacity to produce secondary metabolites, including
antibiotics. In this work, a proteomic approach was applied to the
systems study of germination as a transition from dormancy to the
metabolically active stage. The protein expression levels were examined
throughout the germination time course, the kinetics of the accumulated
and newly synthesized proteins were clustered, and proteins detected
in each group were identified. Altogether, 104 2DE gel images at 13
time points, from dormant state until 5.5 h of growth, were analyzed.
The mass spectrometry identified proteins were separated into functional
groups and their potential roles during germination were further assessed.
The results showed that the full competence of spores to effectively
undergo active metabolism is derived from the sporulation step, which
facilitates the rapid initiation of global protein expression during
the first 10 min of cultivation. Within the first hour, the majority
of proteins were synthesized. From this stage, the full capability
of regulatory mechanisms to respond to environmental cues is presumed.
The obtained results might also provide a data source for further
investigations of the process of germination
Systems Insight into the Spore Germination of <i>Streptomyces coelicolor</i>
An example of bacterium, which undergoes a complex development,
is the genus of <i>Streptomyces</i> whose importance lies
in their wide capacity to produce secondary metabolites, including
antibiotics. In this work, a proteomic approach was applied to the
systems study of germination as a transition from dormancy to the
metabolically active stage. The protein expression levels were examined
throughout the germination time course, the kinetics of the accumulated
and newly synthesized proteins were clustered, and proteins detected
in each group were identified. Altogether, 104 2DE gel images at 13
time points, from dormant state until 5.5 h of growth, were analyzed.
The mass spectrometry identified proteins were separated into functional
groups and their potential roles during germination were further assessed.
The results showed that the full competence of spores to effectively
undergo active metabolism is derived from the sporulation step, which
facilitates the rapid initiation of global protein expression during
the first 10 min of cultivation. Within the first hour, the majority
of proteins were synthesized. From this stage, the full capability
of regulatory mechanisms to respond to environmental cues is presumed.
The obtained results might also provide a data source for further
investigations of the process of germination
Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples Searching for Biomarkers with Potential to Predict the Outcome of Therapy
Determination of the prognosis and treatment outcomes
of dilated
cardiomyopathy is a serious problem due to the lack of valid specific
protein markers. Using in-depth proteome discovery analysis, we compared
49 plasma samples from patients suffering from dilated cardiomyopathy
with plasma samples from their healthy counterparts. In total, we
identified 97 proteins exhibiting statistically significant dysregulation
in diseased plasma samples. The functional enrichment analysis of
differentially expressed proteins uncovered dysregulation in biological
processes like inflammatory response, wound healing, complement cascade,
blood coagulation, and lipid metabolism in dilated cardiomyopathy
patients. The same proteome approach was employed in order to find
protein markers whose expression differs between the patients well-responding
to therapy and nonresponders. In this case, 45 plasma proteins revealed
statistically significant different expression between these two groups.
Of them, fructose-1,6-bisphosphate aldolase seems to be a promising
biomarker candidate because it accumulates in plasma samples obtained
from patients with insufficient treatment response and with worse
or fatal outcome. Data are available via ProteomeXchange with the
identifier PXD046288
Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples Searching for Biomarkers with Potential to Predict the Outcome of Therapy
Determination of the prognosis and treatment outcomes
of dilated
cardiomyopathy is a serious problem due to the lack of valid specific
protein markers. Using in-depth proteome discovery analysis, we compared
49 plasma samples from patients suffering from dilated cardiomyopathy
with plasma samples from their healthy counterparts. In total, we
identified 97 proteins exhibiting statistically significant dysregulation
in diseased plasma samples. The functional enrichment analysis of
differentially expressed proteins uncovered dysregulation in biological
processes like inflammatory response, wound healing, complement cascade,
blood coagulation, and lipid metabolism in dilated cardiomyopathy
patients. The same proteome approach was employed in order to find
protein markers whose expression differs between the patients well-responding
to therapy and nonresponders. In this case, 45 plasma proteins revealed
statistically significant different expression between these two groups.
Of them, fructose-1,6-bisphosphate aldolase seems to be a promising
biomarker candidate because it accumulates in plasma samples obtained
from patients with insufficient treatment response and with worse
or fatal outcome. Data are available via ProteomeXchange with the
identifier PXD046288
Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples Searching for Biomarkers with Potential to Predict the Outcome of Therapy
Determination of the prognosis and treatment outcomes
of dilated
cardiomyopathy is a serious problem due to the lack of valid specific
protein markers. Using in-depth proteome discovery analysis, we compared
49 plasma samples from patients suffering from dilated cardiomyopathy
with plasma samples from their healthy counterparts. In total, we
identified 97 proteins exhibiting statistically significant dysregulation
in diseased plasma samples. The functional enrichment analysis of
differentially expressed proteins uncovered dysregulation in biological
processes like inflammatory response, wound healing, complement cascade,
blood coagulation, and lipid metabolism in dilated cardiomyopathy
patients. The same proteome approach was employed in order to find
protein markers whose expression differs between the patients well-responding
to therapy and nonresponders. In this case, 45 plasma proteins revealed
statistically significant different expression between these two groups.
Of them, fructose-1,6-bisphosphate aldolase seems to be a promising
biomarker candidate because it accumulates in plasma samples obtained
from patients with insufficient treatment response and with worse
or fatal outcome. Data are available via ProteomeXchange with the
identifier PXD046288
Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples Searching for Biomarkers with Potential to Predict the Outcome of Therapy
Determination of the prognosis and treatment outcomes
of dilated
cardiomyopathy is a serious problem due to the lack of valid specific
protein markers. Using in-depth proteome discovery analysis, we compared
49 plasma samples from patients suffering from dilated cardiomyopathy
with plasma samples from their healthy counterparts. In total, we
identified 97 proteins exhibiting statistically significant dysregulation
in diseased plasma samples. The functional enrichment analysis of
differentially expressed proteins uncovered dysregulation in biological
processes like inflammatory response, wound healing, complement cascade,
blood coagulation, and lipid metabolism in dilated cardiomyopathy
patients. The same proteome approach was employed in order to find
protein markers whose expression differs between the patients well-responding
to therapy and nonresponders. In this case, 45 plasma proteins revealed
statistically significant different expression between these two groups.
Of them, fructose-1,6-bisphosphate aldolase seems to be a promising
biomarker candidate because it accumulates in plasma samples obtained
from patients with insufficient treatment response and with worse
or fatal outcome. Data are available via ProteomeXchange with the
identifier PXD046288
Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples Searching for Biomarkers with Potential to Predict the Outcome of Therapy
Determination of the prognosis and treatment outcomes
of dilated
cardiomyopathy is a serious problem due to the lack of valid specific
protein markers. Using in-depth proteome discovery analysis, we compared
49 plasma samples from patients suffering from dilated cardiomyopathy
with plasma samples from their healthy counterparts. In total, we
identified 97 proteins exhibiting statistically significant dysregulation
in diseased plasma samples. The functional enrichment analysis of
differentially expressed proteins uncovered dysregulation in biological
processes like inflammatory response, wound healing, complement cascade,
blood coagulation, and lipid metabolism in dilated cardiomyopathy
patients. The same proteome approach was employed in order to find
protein markers whose expression differs between the patients well-responding
to therapy and nonresponders. In this case, 45 plasma proteins revealed
statistically significant different expression between these two groups.
Of them, fructose-1,6-bisphosphate aldolase seems to be a promising
biomarker candidate because it accumulates in plasma samples obtained
from patients with insufficient treatment response and with worse
or fatal outcome. Data are available via ProteomeXchange with the
identifier PXD046288