Top BLAST matches of the 8 gene segments from FAV-003 and FAV-009 obtained from NCBI influenza A virus nucleotide database.

<p>Top BLAST matches of the 8 gene segments from FAV-003 and FAV-009 obtained from NCBI influenza A virus nucleotide database.</p

Antigenic characterization of triple reassortant H3N2 viruses isolated from turkeys by hemagglutination-inhibition assay using turkey red blood cells and various reference H3 antisera.

<p>Antigenic characterization of triple reassortant H3N2 viruses isolated from turkeys by hemagglutination-inhibition assay using turkey red blood cells and various reference H3 antisera.</p

Percent similarities in the amino acid residues of the HA0 and HA1 of the three H3N2 isolates from turkeys compared to a prototype cluster IV TR H3N2 strain.

<p>ND = Not done [full HA sequence of A/Mallard duck/MN/79/1979 (H3N4) was not available].</p

Alignment of the H3 HA1 amino acid sequences (without signal peptide).

<p>Amino acids of the HA1 subunit of the three unique turkey isolates, the duck H3N4 vaccine strain, a prototype cluster IV TR H3N2 virus (A/SW/ON/33853/2005) and phylogenetically related isolates A/Sw/QC/1265553/2010 (H3N2) and A/SW/QC/1698-1/2009 (H3N2) were aligned. Residues shown in red, green, blue and purple represent previously identified antigenic sites A, B, C and D respectively. Potential glycosylation sites are underlined.</p

Locations of the amino acid alterations at the major antigenic sites of HA1 molecule in the H3 crystal structure.

<p>(I) Side view of a HA monomer in cartoon format with major antigenic sites A, B, C and D shown in spheres (II, III, IV, and V??amino acid changes identified at the major antigenic sites. The locations of changed amino acids are indicated and colored in red. (VI, VII, VIII, IX, and X) Back view of panels I, II, III, IV, and V, respectively. The view in panels I to V is rotated 180° along Y-axis.TK/ON/FAV-003/2011 vs. DK/MN/1979 (II and VII); TK/ON/FAV-009/2011 vs. DK/MN/1979 (III and VIII); TK/ON/FAV-003/2011 vs. SW/ON/33853/2005 (IV and IX); TK/ON/FAV-009/2011 vs. SW/ON/33853/2005 (V and X).</p

Phylogenetic analysis of the HA and NA genes.

<p>The HA (a) and NA (b) gene segments of the unique TR H3N2 viruses isolated from turkeys were compared with other TR H3N2 viruses from turkey (open circle), quail (open diamond) and pig (open triangles) that were previously sequenced by our laboratory <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032858#pone.0032858-Nfon1" target="_blank">[24]</a>.</p

Influenza A matrix gene real time RT-PCR and virus isolation results for the swab specimens received from the three turkey farms.

<p>ND = not determined.</p

Summary of cross neutralization assay results as determined by IPRVN of TR H3N2 viruses isolated from turkeys using various reference H3 antisera.

<p>ND – Not Done.</p