1 research outputs found
Multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in Bacillus cereus batch cultures.
Based on two staining protocols, DiOC6(3)/
propidium iodide (PI) and RedoxSensor Green (an
indicator of bacterial reductase activity)/PI, multiparameter
flow cytometry and cell sorting has identified
at least four distinguishable physiological states during
batch cultures of Bacillus cereus. Furthermore, dependent
on the position in the growth curve, single cells
gave rise to varying numbers of colonies when sorted
individually onto nutrient agar plates. These growing
colonies derived from a single cell had widely different
lag phases, inferred fromdifferences in colony size.This
further highlights the complex population dynamics
of bacterial monocultures and further demonstrates
that individual bacterial cells in a culture respond in markedly dissimilar ways to the environment, resulting
in a physiologically heterogenous and dynamic
population