2 research outputs found
Real-Time Capture and Visualization of Individual Viruses in Complex Media
Label-free imaging of individual
viruses and nanoparticles directly
in complex solutions is important for virology research and biosensing
applications. A successful visualization technique should be rapid,
sensitive, and inexpensive, while needing minimal sample preparation
or user expertise. Current approaches typically require fluorescent
labeling or the use of an electron microscope, which are expensive
and time-consuming to use. We have developed an imaging technique
for real-time, sensitive, and label-free visualization of viruses
and nanoparticles directly in complex solutions such as serum. By
combining the advantages of a single-particle reflectance imaging
sensor, with microfluidics, we perform real-time digital detection
of individual 100 nm vesicular stomatitis viruses as they bind to
an antibody microarray. Using this approach, we have shown capture
and visualization of a recombinant vesicular stomatitis virus Ebola
model (rVSV-ZEBOV) at 100 PFU/mL in undiluted fetal bovine serum in
less than 30 min
Real-Time Capture and Visualization of Individual Viruses in Complex Media
Label-free imaging of individual
viruses and nanoparticles directly
in complex solutions is important for virology research and biosensing
applications. A successful visualization technique should be rapid,
sensitive, and inexpensive, while needing minimal sample preparation
or user expertise. Current approaches typically require fluorescent
labeling or the use of an electron microscope, which are expensive
and time-consuming to use. We have developed an imaging technique
for real-time, sensitive, and label-free visualization of viruses
and nanoparticles directly in complex solutions such as serum. By
combining the advantages of a single-particle reflectance imaging
sensor, with microfluidics, we perform real-time digital detection
of individual 100 nm vesicular stomatitis viruses as they bind to
an antibody microarray. Using this approach, we have shown capture
and visualization of a recombinant vesicular stomatitis virus Ebola
model (rVSV-ZEBOV) at 100 PFU/mL in undiluted fetal bovine serum in
less than 30 min