Young Entrepreneurship in Philadelphia

A contemporary case study in starting a small business in Philadelphia. The paper focuses on my experiences as an artist and centers on a pop-up exhibition held July 15th 2017

The Lantern, 2017-2018

On Dissociation • Untouchable • After Rocket Man • The Science Fair • Cardinal Rule at Stephen J. Memorial • Quentin & Sylvie • Cabello • The Get Out • Painting Day • Black, White and Grey • Family Pruning • How to Remove a Stain • Becoming Ourselves • Wonderbread U • Overture • Pescadero • Gross • Stage Fright • Lucky Daddy • Sarah • Rumble • Silvermine • The Green Iguana • A Poem for Ghost Children • A Poem for Lost Boys • Mother • Drop of Grease • Don\u27t Wanna be White • I • Amelia Earhart Disappeared Into My Vagina: An Ode to Cunts, Menstrual Cups and All Things Woman • Suburban Summer • Nightmares and Dreams Induced by My Mother • Teacups, Skins, etc. • Three Thoughts About My Bedroom • Dear Siri • 2 Queens (Beyonce in Reference to Sonia Sanchez) • Voyeurs • In Front of the Bathroom Mirror • To a Rose • Howl • Mice • Mirror • Language Accordion Volcano Mouth • Lucky Woman • Butterscotch • To Persephone • Wolf • Notes Never Passed • Topple • Bust • Kyoto • Identity • Sunflower • Tornabuoni Bubbles • Olympia • Decayed Hall • Perspectivehttps://digitalcommons.ursinus.edu/lantern/1186/thumbnail.jp

Median-joining haplotype network (A) of environmental <i>C. laurentii</i> isolates based on concatenated nucleotide sequences of the 5′ end of 18S-SSU, D1/D2 of 28S-LSU, and ITS regions.

<p>The tree represents 103 <i>Cryptococcus</i> spp. isolates from Brazil, Botswana, Canada, Japan, India, and the United States. The seven <i>C. laurentii</i> and three <i>C. flavescens</i> haplotypes are clearly distinguished. The Botswana ancestral haplotype (H4) of <i>C. laurentii</i> is presented and highlighted in yellow. Each circle represents a unique haplotype (H), and the circumference is proportional to haplotype frequency (H1: 44 isolates; H2: 1; H3: 18; H4: 2; H5: 8; H6: 2; H7: 1; H8: 1; H9: 8; H10: 2; H11: 3; H12: 2; H13: 10; H14: 1; outgroup <i>C. albidus</i> CBS 142). Yellow dots represents the number of mutation sites, excluding gaps, between the haplotypes. Black dots (median vectors) are hypothetical missing intermediates. Minimum spanning trees (B) using the goeBURST algorithm confirm the haplotype relationships among <i>C. laurentii</i> isolates determined by median-joining network analysis. The size of the circle corresponds to the number of isolates within that haplotype, and the numbers between haplotypes represent the genetic distance of each haplotype, excluding the gaps. Minimum spanning trees as described in B modified to show the distribution of haplotypes according to the country of origin (C) or environmental source (D).</p

DNA polymorphisms in the ribosomal loci of the 75 <i>C. laurentii</i> environmental isolates.

<p><b><i>S</i></b>: number of polymorphic sites. <b><i>π</i></b>: nucleotide diversity. <b><i>k</i></b>: average number of nucleotide differences per sequence. <b><i>h</i></b>: number of haplotypes. <b><i>H_d</i></b>: haplotype diversity. <b><i>D</i></b><b>, </b><b><i>F_D</i></b><b>, </b><b><i>F_F</i></b><b> and </b><b><i>Fs</i></b>: Tajima's D, Fu and Li's D*, Fu and Li's F* and Fu's Fs, respectively.</p>a<p>: p value<0.05.</p><p>DNA polymorphisms in the ribosomal loci of the 75 <i>C. laurentii</i> environmental isolates.</p

Phylogenetic analysis of 100 environmental <i>Cryptococcus</i> spp. isolates generated by the neighbor-joining, UPGMA, and maximum likelihood methods using partial nucleotide sequences of the (A) internal transcribed spacer (ITS) and (B) concatenated sequences of the three ribosomal regions.

<p>Numbers at each branch indicate bootstrap values>50% based on 1,000 replicates (NJ/UPGMA/ML). The analysis involved 105 and 103 nucleotide sequences for ITS and concatenated sequences respectively. ^T: Type strain.</p

Differential interference contrast (A) and India Ink staining (B) of <i>C. aspenensis</i> sp. nov. DS573^T (CBS 13867) cells after 3 days at 25°C in YPD broth. Scale bar of 20 µm is shown.

<p>Differential interference contrast (A) and India Ink staining (B) of <i>C. aspenensis</i> sp. nov. DS573^T (CBS 13867) cells after 3 days at 25°C in YPD broth. Scale bar of 20 µm is shown.</p

Isolate, species, source, and GenBank accession numbers of <i>Cryptococcus</i> spp. environmental isolates.

<p>NA: Not applicable.</p>T<p>: Type strain. Hap: Haplotype number.</p>#<p>: <i>C. aspenensis</i> sp. nov. (CBS 13867).</p><p>Mopane trees (<i>Colophospermum mopane</i>). Douglas fir (<i>Pseudotsuga menziesii</i>). Norway spruce (<i>Picea abies</i>). Trembling aspen (<i>Populus tremuloides</i>). CBS: Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands. 18S-SSU: Small subunit rDNA. 28S-LSU: Large subunit rDNA. ITS: Internal transcribed spacer region.</p><p>Isolate, species, source, and GenBank accession numbers of <i>Cryptococcus</i> spp. environmental isolates.</p

Phylogenetic analysis of 100 environmental <i>Cryptococcus</i> spp. isolates generated by the neighbor-joining, UPGMA, and maximum likelihood methods using partial nucleotide sequences of the (A) 5′end of 18S SSU-rDNA and (B) D1/D2 region of 28S LSU-rDNA.

<p>Numbers at each branch indicate bootstrap values>50% based on 1,000 replicates (NJ/UPGMA/ML). The analysis involved 103 and 105nucleotide sequences for the 18S-SSU and 28S-LSU respectively. ^T: Type strain.</p

Intraspecific and interspecific pairwise distance of the three ribosomal regions of the environmental <i>Cryptococcus</i> spp. calculated by the Kimura 2-parameter model revealed higher variability of the ITS region compared with the 18S-SSU and 28S-LSU regions.

<p>Intraspecific and interspecific pairwise distance of the three ribosomal regions of the environmental <i>Cryptococcus</i> spp. calculated by the Kimura 2-parameter model revealed higher variability of the ITS region compared with the 18S-SSU and 28S-LSU regions.</p

PCR conditions and primers used for the amplification of the ribosomal loci.

<p>PCR conditions and primers used for the amplification of the ribosomal loci.</p