MOESM2 of Dimerized translationally controlled tumor protein increases interleukin-8 expression through MAPK and NF-κB pathways in a human bronchial epithelial cell line

Additional file 2: Figure S2. Lack of FcεRIα expression in BEAS-2B cells. FcεRIα expression was determined by immunoblotting in BMMC and BEAS-2B cells. For BEAS-2B, 10 μg/ml of dTCTP was treated for the indicated times and the change in protein expression were measured. BMMC was used as a positive control for cells expressing FcεRIα

MOESM4 of Dimerized translationally controlled tumor protein increases interleukin-8 expression through MAPK and NF-κB pathways in a human bronchial epithelial cell line

Additional file 4: Figure S4. Dose-denpendent IL-8 release by dTCTP. BEAS-2B cells were stimulated with the indicated doses of dTCTP (0–10 μg/ml) and incubated for 16 h. The IL-8 protein released into the supernatant was measured using a sandwhich ELISA kit. The relative percentage was calculated by setting the maximum value of IL-8 to 100%

Additional file 1 of Mut2Vec: distributed representation of cancerous mutations

It contains the visualization results with mutation vectors trained with an autoencoder and a denoising autoencoder. (PDF 427 kb

Additional file 2 of Mut2Vec: distributed representation of cancerous mutations

It contains the most enriched clusters with IntOGen driver mutations obtained by six clustering methods(K-Means, Agglomerative hierarchical clustering, BIRCH, Spectral clustering, Affinity Propagation, and Gaussian Mixture) and five options of the number of clusters(50, 100, 200, 300 and 500); except Affinity Propagation. (PDF 108 kb

Solvent-Free Methallylboration of Ketones Accelerated by <i>tert</i>-Alcohols

A solvent- and metal-free process has been developed for the direct methallylboration of ketones employing the stable <i>B</i>-methallylborinane <b>1</b>, which was accelerated by tertiary alcohols. In the presence of 2.0 equiv of readily available tertiary alcohols such as <i>tert</i>-amyl alcohol, the methallylation products were prepared at room temperature in excellent yields. The salient features of the described process include simple operation, high efficiency, and mild reaction conditions

Carbamoyl Anion Addition to Nitrones

The addition of carbamoyl anions derived from <i>N</i>,<i>N</i>-disubstituted formamides and LDA to <i>N</i>-<i>tert</i>-butyl nitrones is described. The reaction was demonstrated with a variety of formamides and nitrones and provided a direct route to α-(<i>N</i>-hydroxy)­amino amides. The use of a <i>tert</i>-leucinol derived chiral auxiliary on the nitrone provided products in good diastereoselectivity. Derivatization of the products by <i>tert</i>-butyl deprotection or <i>N</i>-deoxygenation was demonstrated

The Reaction of Grignard Reagents with Bunte Salts: A Thiol-Free Synthesis of Sulfides

S-Alkyl, S-aryl, and S-vinyl thiosulfate sodium salts (Bunte salts) react with Grignard reagents to give sulfides in good yields. The S-alkyl Bunte salts are prepared from odorless sodium thiosulfate by an S_N2 reaction with alkyl halides. A Cu-catalyzed coupling of sodium thiosulfate with aryl and vinyl halides was developed to access S-aryl and S-vinyl Bunte salts. The reaction is amenable to a broad structural array of Bunte salts and Grignard reagents. Importantly, this route to sulfides avoids the use of malodorous thiol starting materials or byproducts

Metal-Free Cycloetherification by in Situ Generated <i>P</i>‑Stereogenic α‑Diazanium Intermediates: A Convergent Synthesis of Enantiomerically Pure Dihydrobenzooxaphospholes

A metal-free tandem reaction, initiated by the generation of a diazonium cation and followed by cycloetherification, was developed. Acid-promoted de-<i>tert</i>-butylation of <i>N</i>-nitroso <i>N</i>-<i>tert</i>-butylamine was used to generate a diazonium cation in situ, demonstrating a new application of nitroso chemistry. This reaction was employed in the synthesis of substituted benzofuran-3­(2<i>H</i>)-ones and dihydrobenzo­[<i>d</i>]­[1,3]­oxaphosphole 3-oxides

Synthesis of <i>P</i>‑Chiral Dihydrobenzooxaphospholes Through Negishi Cross-Coupling

An efficient Negishi cross-coupling was developed for the synthesis of the biaryl axes present in useful <i>P</i>-chiral dihydrobenzooxaphosphole ligands. This approach has allowed for the synthesis of new derivatives of these ligands that were not accessible by the previous route employing Suzuki–Miyaura cross-coupling. The use of Pd₂(dba)₃/<b>BI-DIME</b> as the catalyst system affords the desired biaryl compounds in good yields with excellent rates and with catalyst loadings as low as 0.25 mol %

Copper-Catalyst-Controlled Site-Selective Allenylation of Ketones and Aldehydes with Propargyl Boronates

A practical and highly site-selective copper-PhBPE-catalyst-controlled allenylation with propargyl boronates has been developed. The methodology has shown to be tolerant of diverse ketones and aldehydes providing the allenyl adducts in high selectivity. The BPE ligand and boronate substituents were shown to direct the site selectivity for which either propargyl or allenyl adducts can be acquired in high selectivity. A model is proposed that explains the origin of the site selectivity