Three-Dimensional Superlocalization Imaging of Gliding <i>Mycoplasma mobile</i> by Extraordinary Light Transmission through Arrayed Nanoholes

In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding <i>Mycoplasma mobile</i> (<i>M. mobile</i>). The results analyzed with multiple <i>M. mobile</i> bacteria show individual characters and reveal that <i>M. mobile</i> undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision

Three-Dimensional Superlocalization Imaging of Gliding <i>Mycoplasma mobile</i> by Extraordinary Light Transmission through Arrayed Nanoholes

In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding <i>Mycoplasma mobile</i> (<i>M. mobile</i>). The results analyzed with multiple <i>M. mobile</i> bacteria show individual characters and reveal that <i>M. mobile</i> undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision

Three-Dimensional Superlocalization Imaging of Gliding <i>Mycoplasma mobile</i> by Extraordinary Light Transmission through Arrayed Nanoholes

In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding <i>Mycoplasma mobile</i> (<i>M. mobile</i>). The results analyzed with multiple <i>M. mobile</i> bacteria show individual characters and reveal that <i>M. mobile</i> undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision

Three-Dimensional Superlocalization Imaging of Gliding <i>Mycoplasma mobile</i> by Extraordinary Light Transmission through Arrayed Nanoholes

In this paper, we describe super-resolved sampling of live bacteria based on extraordinary optical transmission (EOT) of light. EOT is produced by surface plasmon confinement and coupling with nanostructures. Bacterial fluorescence is excited by the localized fields for subdiffraction-limited sampling. The concept was applied to elucidating bacterial dynamics of gliding <i>Mycoplasma mobile</i> (<i>M. mobile</i>). The results analyzed with multiple <i>M. mobile</i> bacteria show individual characters and reveal that <i>M. mobile</i> undergoes a significant axial variation at 94 nm. The sampling error of the method is estimated to be much smaller than 1/10 of the diffraction limit both in the lateral and depth axis. The method provides a powerful tool for investigation of biomolecular dynamics at subwavelength precision

Systematic Study on the Sensitivity Enhancement in Graphene Plasmonic Sensors Based on Layer-by-Layer Self-Assembled Graphene Oxide Multilayers and Their Reduced Analogues

The use of graphene in conventional plasmonic devices was suggested by several theoretic research studies. However, the existing theoretic studies are not consistent with one another and the experimental studies are still at the initial stage. To reveal the role of graphenes on the plasmonic sensors, we deposited graphene oxide (GO) and reduced graphene oxide (rGO) thin films on Au films and their refractive index (RI) sensitivity was compared for the first time in SPR-based sensors. The deposition of GO bilayers with number of deposition L from 1 to 5 was carried out by alternative dipping of Au substrate in positively- and negatively charged GO solutions. The fabrication of layer-by-layer self-assembly of the graphene films was monitored in terms of the SPR angle shift. GO-deposited Au film was treated with hydrazine to reduce the GO. For the rGO-Au sample, 1 bilayer sample showed a higher RI sensitivity than bare Au film, whereas increasing the rGO film from 2 to 5 layers reduced the RI sensitivity. In the case of GO-deposited Au film, the 3 bilayer sample showed the highest sensitivity. The biomolecular sensing was also performed for the graphene multilayer systems using BSA and anti-BSA antibody