HOW POLICY DECISIONS ARE MADE IN CANADIAN AGRICULTURE

Agricultural and Food Policy,

Query-related data extraction of hidden web documents

The larger amount of information on the Web is stored in document databases and is not indexed by general-purpose search engines (i.e., Google and Yahoo). Such information is dynamically generated through querying databases — which are referred to as Hidden Web databases. Documents returned in response to a user query are typically presented using templategenerated Web pages. This paper proposes a novel approach that identifies Web page templates by analysing the textual contents and the adjacent tag structures of a document in order to extract query-related data. Preliminary results demonstrate that our approach effectively detects templates and retrieves data with high recall and precision

Information extraction from template-generated hidden web documents

The larger amount of information on the Web is stored in document databases and is not indexed by general-purpose search engines (such as Google and Yahoo). Databases dynamically generate a list of documents in response to a user query – which are referred to as Hidden Web databases. Such documents are typically presented to users as templategenerated Web pages. This paper presents a new approach that identifies Web page templates in order to extract queryrelated information from documents. We propose two forms of representation to analyse the content of a document – Text with Immediate Adjacent Tag Segments (TIATS) and Text with Neighbouring Adjacent Tag Segments (TNATS). Our techniques exploit tag structures that surround the textual contents of documents in order to detect Web page templates thereby extracting query-related information. Experimental results demonstrate that TNATS detects Web page templates most effectively and extracts information with high recall and precision

Transcriptomic profiling of human breast and melanoma cells selected by migration through narrow constraints

The metastatic spread of cancer cells is a step-wise process that starts with dissociation from primary tumours and local invasion of adjacent tissues. The ability to invade local tissues is the product of several processes, including degradation of extracellular matrices (ECM) and movement of tumour cells through physically-restricting gaps. To identify properties contributing to tumour cells squeezing through narrow gaps, invasive MDA-MB-231 human breast cancer and MDA-MB-435 human melanoma cells were subjected to three successive rounds of selection using cell culture inserts with highly constraining 3 μm pores. For comparison purposes, flow cytometry was also employed to enrich for small diameter MDA-MB-231 cells. RNA-Sequencing (RNA-seq) using the Illumina NextSeq 500 platform was undertaken to characterize how gene expression differed between parental, invasive pore selected or small diameter cells. Gene expression results obtained by RNA-seq were validated by comparing with RT-qPCR. Transcriptomic data generated could be used to determine how alterations that enable cell passage through narrow spaces contribute to local invasion and metastasis

Calcium signalling links MYC to NUAK1

NUAK1 is a member of the AMPK-related family of kinases. Recent evidence suggests that NUAK1 is an important regulator of cell adhesion and migration, cellular and organismal metabolism, and regulation of TAU stability. As such, NUAK1 may play key roles in multiple diseases ranging from neurodegeneration to diabetes and metastatic cancer. Previous work revealed a crucial role for NUAK1 in supporting viability of tumour cells specifically when MYC is overexpressed. This role is surprising, given that NUAK1 is activated by the tumour suppressor LKB1. Here we show that, in tumour cells lacking LKB1, NUAK1 activity is maintained by an alternative pathway involving calcium-dependent activation of PKCα. Calcium/PKCα-dependent activation of NUAK1 supports engagement of the AMPK-TORC1 metabolic checkpoint, thereby protecting tumour cells from MYC-driven cell death, and indeed, MYC selects for this pathway in part via transcriptional regulation of PKCα and ITPR. Our data point to a novel role for calcium in supporting tumour cell viability and clarify the synthetic lethal interaction between NUAK1 and MYC

Screening and mortality from cervical cancer. Study shows importance of centralised organisation in screening.

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