A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus

Bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as Bacillus sp. YAS 1 after biochemical and molecular identifications. A sequential statistical approach (Plackett-Burman and Box-Behnken) was employed to optimize bacteriocin (BAC YAS 1) production. Using optimal levels of three key determinants (yeast extract (0.48% (w/v), incubation time (62 hrs), and agitation speed (207 rpm)) in peptone yeast beef based production medium resulted in 1.6-fold enhancement in BAC YAS 1 level (470 AU/mL arbitrary units against Erwinia amylovora). BAC YAS 1 showed activity over a wide range of pH (1–13) and temperature (45–80°C). A wide spectrum antimicrobial activity of BAC YAS 1 against the human pathogens (Clostridium perfringens, Staphylococcus epidermidis, Campylobacter jejuni, Enterobacter aerogenes, Enterococcus sp., Proteus sp., Klebsiella sp., and Salmonella typhimurium), the plant pathogen (E. amylovora), and the food spoiler (Listeria innocua) was demonstrated. On top and above, BAC YAS 1 showed no antimicrobial activity towards lactic acid bacteria (Lactobacillus bulgaricus, L. casei, L. lactis, and L. reuteri). Promising characteristics of BAC YAS 1 prompt its commercialization for efficient utilization in several industries

Effects of Using Green Concrete Materials on the CO2 Emissions of the Residential Building Sector in Egypt

Increasing the rate of construction material consumption has caused significant environmental problems in recent decades, especially the production of ordinary Portland cement (OPC), which has been associated with 8% of the world’s human CO2 emissions and is considered the leading binder of concrete. This study aims to investigate the effects of substituting conventional concrete (CC) material with green concrete (GC) in the non-structural concrete works of a residential building in New Borg El-Arab City, Egypt. It attempts to establish what the effects are of using GC on cement, natural aggregates, and CO2 emissions in the design phase. By using a design-based solution (DBS), we began with redesign, reduce, reselect, reuse, and recycle strategies to find an optimal solution for applying recycle aggregate concrete (RAC) as a replacement material in selected building parts, such as the internal floor, external sidewalk, entrance steps, and wall boundary. AutoCAD software and 3Dmax were used to modify the original design and obtain two design references with four different scenarios. Comparative analyses were applied to investigate the effects of different concrete materials. The results show a reduction of about 19.4% in cement consumption in terms of the total concrete of the building and a 44.5% reduction in CO2 emissions due to the reduction of cement in specific building parts. In addition, this solution decreased natural coarse aggregate (NCA) consumption by 23.7% in the final concrete. This study recommends that GC materials close the loop of cementitious material consumption to reduce environmental impacts and achieve sustainability in the Egyptian building sector

Use of Wheat Straw for Value-Added Product Xylanase by Penicillium chrysogenum Strain A3 DSM105774

The present work highlights the valorization of the bulky recalcitrant lignocellulose byproduct wheat straw (WS) for the enhanced production of value-added xylanase by the locally sourced novel Penicillium chrysogenum strain A3 DSM105774 for the first time. The optimized production of xylanase by submerged state of fermentation of WS was achieved using a three-step statistical and sequential approach: one factor at a time (OFAT), Plackett–Burman design (PBD), and Box Behnken design (BBD). Incubation temperature (30 °C), WS, and ammonium sulphate were the key determinants prompting xylanase production; inferred from OFAT. The WS concentration (%(w/v)), yeast extract concentration (%(w/v)), and initial pH of the production medium imposed significant effects (p ≤ 0.05) on the produced xylanase, realized from PBD. The predicted levels of WS concentration, initial pH of the production medium, and yeast extract concentration provoking the ultimate xylanase levels (53.7 U/mL) with an 8.95-fold enhancement, localized by the estimated ridge of the steepest ascent of the ridge analysis path, were 3.8% (w/v), 5.1, and 0.098% (w/v), respectively; 94.7% lab validation. The current data underpin the up-scaling of xylanase production using this eco-friendly, cheap, and robust methodology for the valorization of WS into the value-added product xylanase

A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation.

Pectic-Oligosaccharides (POS) have a growing potential in food and feed industries. To satisfy the demand of worldwide markets from POS and avoid the shortcomings of currently applied methodologies encountered in their preparation, the present study highlights a novel robust approach for POS biosynthesis. In the current approach, Aspergillus sp.section Flavi strain EGY1 DSM 101520 was grown on citrus pectin-based medium as a core POS production medium. POS' levels accumulated in the fungal fermentation broth were optimized through a three step sequential statistical mathematical methodology; Plackett-Burman design (PBD), Box-Behnken design (BBD) and canonical analysis. Three key determinants namely citrus pectin, peptone and NaH2PO4 were pointed out by PBD to impose significant consequences (P<0.05) on the process outcome (POS' levels). Optimal levels of these key determinants along with maximal of POS' levels were set by BBD and canonical analysis to be 2.28% (w/v) citrus pectin, 0.026% (w/v) peptone and 0.28% (w/v) NaH2PO4 to achieve a net amount of 1.3 g POS /2.28 g citrus pectin. Through this approach, a yield of 57% (w/w) POS of the total citrus pectin was obtained after 24 h of fungal growth on optimized citrus pectin-based medium. A fold enhancement of 13 times in POS' levels released in the fermentation fungal broth was realized by the end of the optimization strategy. This novel robust approach is considered a new insight towards POS biosynthesis via efficient, rapid and non-cumbersome procedure. To the best of authors' knowledge, the present work is the first article underlining detailed POS production from the fermentation broth of a fungus growing on citrus pectin-based medium

Three dimensional surface plot for the dependent variable POS in terms of reducing sugars vs. the independent variables peptone and NaH₂PO₄ at constant optimal value of pectin.

<p>Three dimensional surface plot for the dependent variable POS in terms of reducing sugars vs. the independent variables peptone and NaH₂PO₄ at constant optimal value of pectin.</p

Three dimensional surface plot for the dependent variable POS in terms of reducing sugars vs. the independent variables pectin and peptone at constant optimal value of NaH₂PO₄.

<p>Three dimensional surface plot for the dependent variable POS in terms of reducing sugars vs. the independent variables pectin and peptone at constant optimal value of NaH₂PO₄.</p

Real-coded values of independent variables and regression analysis for POS' optimization by PBD.

<p>Real-coded values of independent variables and regression analysis for POS' optimization by PBD.</p

Real–coded values of independent variables and regression analysis for POS optimization by Box-Behnken.

<p>Real–coded values of independent variables and regression analysis for POS optimization by Box-Behnken.</p

Estimated maximum response (POS) production by <i>Aspergillus</i> sp. section Flavi strain EGY1 DSM 101520 using a canonical path.

<p>Estimated maximum response (POS) production by <i>Aspergillus</i> sp. section Flavi strain EGY1 DSM 101520 using a canonical path.</p

Coded-Real levels of six independent variables in PBD for optimizing of POS' production by <i>Aspergillus</i> sp. section Flavi strain EGY1 DSM 101520 along with experimental and predicted levels of releasing POS.

<p>Coded-Real levels of six independent variables in PBD for optimizing of POS' production by <i>Aspergillus</i> sp. section Flavi strain EGY1 DSM 101520 along with experimental and predicted levels of releasing POS.</p