A Qualitative Investigation of a Setting-Wide PBS Workforce Development Programme in an Adult Disability Setting

The presence of distressed behaviours can amplify the difficulties experienced by people with intellectual disabilities (ID), and place pressure on the provision of effective support by organisations and direct support personnel. Setting-wide positive behaviour support (PBS) is an evidence-based framework aimed at enhancing quality of life and reducing distressed behaviour for people with intellectual disabilities through systemic change. Implementation science offers a route to better understand how we can support organisations to adopt best practice into routine procedures. This study employed a qualitative research design to examine the facilitators and barriers of a workforce development programme in setting-wide PBS in a disability service organisation through semi-structured interviews with staff (n=14). Two conceptual models of implementation science, the theoretical domain framework and the Capability, Opportunity, and Motivation (COM-B) Behaviour change model were adopted to code and analyse participant responses. Positive relationships with adults with ID, effective leadership and supervision and the systemic components of the PBS framework were highlighted as important enablers for behaviour change among participants. Workload, staff turnover, limited resources including lack of investment in workforce development and lack of mentorship were among the barriers identified. Systemic concerns with staff wellbeing, retention and capacity development continue to be prevalent in disability service provision. Outcomes indicate a need for significant investment in widespread, mandatory evidence-based training programmes in quality support for direct support staff, first line managers and service leaders

Mapping Glacier Ablation With a UAV in the North Cascades: A Structure-from-Motion Approach

The glaciers of the North Cascades have experienced mass loss and terminus retreat due to climate change. The meltwater from these glaciers provides a flux of cold glacier meltwater into the river systems, which supports salmon spawning during the late summer dry season. The Nooksack Indian Tribe monitors the outlet flow of the Sholes Glacier within the North Cascades range with the goal of understanding the health of the glacier and the ability of the Tribe to continue to harvest sustainable populations of salmon. This study compares the UAV derived glacier ablation with the discharge data collected by the Tribe. We surveyed the Sholes Glacier twice throughout the 2020 melt season and, using Structure-from-Motion technology, generated high resolution multispectral orthomosaics and Digital Elevation Models (DEMs) of the glacier on each of the survey dates. The DEMs were differenced to reveal the surface height change of the glacier. The spectral data of the orthomosaics were used to conduct IsoData unsupervised classification. This process divided the survey area into Snow, Ice, and Rock classes that were then used to attribute the surface height changes of the DEMs to either snow or ice melt. The analysis revealed the glacier lost an average thickness of −0.132 m per day (m d−1) with snow and ice losing thickness at similar rates, −0.130 m d−1 and −0.132 m d−1 respectively. DEM differencing reveals that a total of −550,161 ± 45,206 m3 water equivalent (w.e.) was discharged into Wells Creek between the survey dates whereas the stream gauge station measured a total discharge of 350,023 m3. This study demonstrates the ability to spectrally classify the UAV data and derive discharge measurements while evaluating the small-scale spatial variability of glacier melt. Assessing ablation in small alpine glaciers is of great importance to downstream communities, like the Nooksack Indian Tribe who seek to understand the magnitude and timing of glacier melt in order to better protect their salmon populations. With this paper, we provide a baseline for future glacier monitoring and the potential to connect the snow surface properties with the rate of snow melt into a warming future

Cell-to-cell diversity in protein levels of a gene driven by a tetracycline inducible promoter

<p>Abstract</p> <p>Background</p> <p>Gene expression in <it>Escherichia coli </it>is regulated by several mechanisms. We measured in single cells the expression level of a single copy gene coding for green fluorescent protein (GFP), integrated into the genome and driven by a tetracycline inducible promoter, for varying induction strengths. Also, we measured the transcriptional activity of a tetracycline inducible promoter controlling the transcription of a RNA with 96 binding sites for MS2-GFP.</p> <p>Results</p> <p>The distribution of GFP levels in single cells is found to change significantly as induction reaches high levels, causing the Fano factor of the cells' protein levels to increase with mean level, beyond what would be expected from a Poisson-like process of RNA transcription. In agreement, the Fano factor of the cells' number of RNA molecules target for MS2-GFP follows a similar trend. The results provide evidence that the dynamics of the promoter complex formation, namely, the variability in its duration from one transcription event to the next, explains the change in the distribution of expression levels in the cell population with induction strength.</p> <p>Conclusions</p> <p>The results suggest that the open complex formation of the tetracycline inducible promoter, in the regime of strong induction, affects significantly the dynamics of RNA production due to the variability of its duration from one event to the next.</p

Comparison of MSIS and Jacchia atmospheric density models for orbit determination and propagation

Two atmospheric density model families that are commonly chosen for orbit determination and propagation, Jacchia and MSIS, are compared for accuracy. The Jacchia 70 model, the MSISE-90 model, and the NRLMSISE-00 model may each be used to determine orbits over fitspans of several days and then to propagate forward. With observations kept over the propagation period, residuals may be computed and the accuracy of each model evaluated. We have performed this analysis for over 4000 cataloged satellites with perigee below 1000km for September-­October 1999, and the 60 HASDM calibration satellites with a large observation set for February 2001. The purpose of this study is to form a picture of the relative merits of the drag models in a comprehensive view, using all satellites in a manner consistent with the operational practice of US space surveillance centers. A further goal is to refine this knowledge to understand the orbital parameter regions where one of the models may be consistently superior

Non-Invasive Assessment of Back Surface Topography: Technologies, Techniques and Clinical Utility

(1) Background: Frequent exposure to ionising radiation is often used to determine the diagnosis of adolescent idiopathic scoliosis (AIS), a lateral curvature of the spine in those aged between 10 and 18 years, and a treatment plan according to Cobb angle. This narrative review outlines the clinical utility of surface topography (ST), a radiation-free imaging modality. (2) Methods: Publicly available databases were searched to yield literature related to ST. Identified articles were classified based on the equipment used and in order of how it was developed, i.e., historical, recent developments, and state-of-the-art developments. (3) Conclusions: ST is a reliable cost-effective non-invasive technique that provides an alternative to radiation-based imaging to aid with the diagnosis and potential screening of AIS. Several scanning methods are available, which allows ST to be used in several clinical environments. Limitations of inter-reliability and differences of apparatus resulting in variations of data have been noted through this narrative review

The Simons Observatory: Magnetic Sensitivity Measurements of Microwave SQUID Multiplexers

The Simons Observatory (SO) will be a cosmic microwave background (CMB) survey experiment with three small-aperture telescopes and one large-aperture telescope, which will observe from the Atacama Desert in Chile. In total, SO will field $\sim$70,000 transition-edge sensor (TES) bolometers in six spectral bands centered between 27 and 280 GHz in order to achieve the sensitivity necessary to measure or constrain numerous cosmological quantities. The SO Universal Focal Plane Modules (UFMs) each contain a 150 mm diameter TES detector array, horn or lenslet optical coupling, cold readout components, and magnetic shielding. SO will use a microwave SQUID multiplexing ($\mu$MUX) readout at an initial multiplexing factor of $\sim$1000; the cold (100 mK) readout components are packaged in a $\mu$MUX readout module, which is part of the UFM, and can also be characterized independently. The 100 mK stage TES bolometer arrays and microwave SQUIDs are sensitive to magnetic fields, and their measured response will vary with the degree to which they are magnetically shielded. We present measurements of the magnetic pickup of test microwave SQUID multiplexers as a study of various shielding configurations for the Simons Observatory. We discuss how these measurements motivated the material choice and design of the UFM magnetic shielding.Comment: 5 pages, 6 figures, conference proceedings submitted to IEEE Transactions on Applied Superconductivit

Single cell transcriptome analysis reveals disease-defining T cell subsets in the tumor microenvironment of classic Hodgkin lymphoma

Hodgkin lymphoma is characterized by an extensively dominant tumor microenvironment (TME) composed of different types of noncancerous immune cells with rare malignant cells. Characterization of the cellular components and their spatial relationship is crucial to understanding cross-talk and therapeutic targeting in the TME. We performed single-cell RNA sequencing of more than 127,000 cells from 22 Hodgkin lymphoma tissue specimens and 5 reactive lymph nodes, profiling for the first time the phenotype of the Hodgkin lymphoma–specific immune microenvironment at single-cell resolution. Single-cell expression profiling identified a novel Hodgkin lymphoma–associated subset of T cells with prominent expression of the inhibitory receptor LAG3, and functional analyses established this LAG3+ T-cell population as a mediator of immunosuppression. Multiplexed spatial assessment of immune cells in the microenvironment also revealed increased LAG3+ T cells in the direct vicinity of MHC class II–deficient tumor cells. Our findings provide novel insights into TME biology and suggest new approaches to immune-checkpoint targeting in Hodgkin lymphoma. SIGNIFICANCE: We provide detailed functional and spatial characteristics of immune cells in classic Hodgkin lymphoma at single-cell resolution. Specifically, we identified a regulatory T-cell–like immunosuppressive subset of LAG3+ T cells contributing to the immune-escape phenotype. Our insights aid in the development of novel biomarkers and combination treatment strategies targeting immune checkpoints

Effects of Transcriptional Pausing on Gene Expression Dynamics

Stochasticity in gene expression affects many cellular processes and is a source of phenotypic diversity between genetically identical individuals. Events in elongation, particularly RNA polymerase pausing, are a source of this noise. Since the rate and duration of pausing are sequence-dependent, this regulatory mechanism of transcriptional dynamics is evolvable. The dependency of pause propensity on regulatory molecules makes pausing a response mechanism to external stress. Using a delayed stochastic model of bacterial transcription at the single nucleotide level that includes the promoter open complex formation, pausing, arrest, misincorporation and editing, pyrophosphorolysis, and premature termination, we investigate how RNA polymerase pausing affects a gene's transcriptional dynamics and gene networks. We show that pauses' duration and rate of occurrence affect the bursting in RNA production, transcriptional and translational noise, and the transient to reach mean RNA and protein levels. In a genetic repressilator, increasing the pausing rate and the duration of pausing events increases the period length but does not affect the robustness of the periodicity. We conclude that RNA polymerase pausing might be an important evolvable feature of genetic networks