1 research outputs found
Microfluidic Device-Based <i>In Vivo</i> Detection of PD-L1-Positive Small Extracellular Vesicles and Its Application for Tumor Monitoring
Liquid biopsy is of great significance in tumor early
diagnosis
and treatment stratification. PD-L1-positive small extracellular vesicles
(PD-L1+ sEVs) are closely related to tumor growth and immunotherapy
response, which are considered valuable liquid biopsy biomarkers.
In contrast to conventional in vitro detection, in vivo detection has the ability to improve the detection
efficiency and enable continuous or real-time dynamic monitoring.
However, in vivo detection of PD-L1+ sEVs
has multiple difficulties, such as high cell background, complex blood
environments, and lack of a specific and stable detection method.
Herein, the in vivo detection of PD-L1+ sEVs method was constructed, which efficiently separated sEVs based
on the microfluidic device and quantitatively analyzed PD-L1+ sEVs by aptamer recognition and hybridization chain reaction. The
concentration of PD-L1+ sEVs was continuously monitored,
and significant differences at different stages of tumor as well as
a correlation with tumor volume were found. Diseased and healthy individuals
could also be effectively distinguished based on the concentration
of PD-L1+ sEVs. The method with good stability, biocompatibility,
and detection performance provided a powerful means for in
vivo detection of PD-L1+ sEVs, contributing to
the clinical diagnosis and treatment of tumor