177 research outputs found
Effect of elevation on composition and diversity of fungi in the rhizosphere of a population of Deyeuxia angustifolia on Changbai Mountain, northeastern China
Soil fungi are a key component of terrestrial ecosystems and play a major role in soil biogeochemical cycling. Although the diversity and composition of fungal communities are regulated by many abiotic and biotic factors, the effect of elevation on soil fungal community diversity and composition remains largely unknown. In this study, the soil fungal composition and diversity in Deyeuxia angustifolia populations along an elevational gradient (1,690 m to 2020 m a.s.l.) were assessed, using Illumina MiSeq sequencing, on the north-facing slope of the Changbai Mountain, northeastern China. Our results showed that soil physicochemical parameters changed significantly along with the elevational gradients. The Ascomycota and Basidiomycota were the most dominant phyla along with the gradient. Alpha diversity of soil fungi decreased significantly with elevation. Soil nitrate nitrogen (NO3−-N) was positively correlated with fungal richness and phylogenetic diversity (PD), indicating that soil nitrate nitrogen (NO3−-N) is a key soil property determining fungal community diversity. In addition to soil nitrate content, soil pH and soil moisture were the most important environmental properties determining the soil fungal diversity. Our results suggest that the elevational changes in soil physicochemical properties play a key role in shaping the community composition and diversity of soil fungi. This study will allow us to better understand the biodiversity distribution patterns of soil microorganisms in mountain ecosystems
Prevalence of porcine circovirus-like agent P1 in Jiangsu, China
Recently, we identified a novel porcine circovirus type 2-like agent P1 isolate from swine. The present study represents the first survey of P1 prevalence in swine herds from Jiangsu, China, by using PCR targeting the complete genome of P1. Prevalences of 50% and 19% were found among 6 herds and 248 animals, respectively. The results indicate a high prevalence of P1 in China pig populations
Independent evolution of sex chromosomes and male pregnancy-related genes in two seahorse species
Robust 3.7 V-Na[CuMn]O Cathode for Na-ion Batteries
Na-ion batteries (NIBs), which are recognized as a next-generation
alternative technology for energy storage, still suffer from commercialization
constraints due to the lack of low-cost, high-performance cathode materials.
Since our first discovery of Cu/Cu electrochemistry in 2014,
numerous Cu-substituted/doped materials have been designed for NIBs. However
for almost ten years, the potential of Cu/Cu electrochemistry has
been grossly underappreciated and normally regarded as a semielectrochemically
active redox. Here, we re-synthesized P2-Na[CuMn]O
and reinterpreted it as a high-voltage, cost-efficient, air-stable, long-life,
and high-rate cathode material for NIBs, which demonstrates a high operating
voltage of 3.7 V and a completely active Cu/Cu redox reaction.
The 2.3 Ah cylindrical cells exhibit excellent cycling (93.1% capacity after
2000 cycles), high rate (97.2% capacity at 10C rate), good low-temperature
performance (86.6% capacity at -30C), and high safety, based on which,
a 56 V-11.5 Ah battery pack for E-bikes is successfully constructed, exhibiting
stable cycling (96.5% capacity at the 800th cycle) and a long driving distance
(36 km, tester weight 65 kg). This work offers a commercially feasible cathode
material for low-cost, high-voltage NIBs, paving the way for advanced NIBs in
power and stationary energy storage applications.Comment: 15 pages, 3 figures, 1 tabl
Frequent alterations in cytoskeleton remodelling genes in primary and metastatic lung adenocarcinomas
The landscape of genetic alterations in lung adenocarcinoma derived from Asian patients is largely uncharacterized. Here we present an integrated genomic and transcriptomic analysis of 335 primary lung adenocarcinomas and 35 corresponding lymph node metastases from Chinese patients. Altogether 13 significantly mutated genes are identified, including the most commonly mutated gene TP53 and novel mutation targets such as RHPN2, GLI3 and MRC2. TP53 mutations are furthermore significantly enriched in tumours from patients harbouring metastases. Genes regulating cytoskeleton remodelling processes are also frequently altered, especially in metastatic samples, of which the high expression level of IQGAP3 is identified as a marker for poor prognosis. Our study represents the first large-scale sequencing effort on lung adenocarcinoma in Asian patients and provides a comprehensive mutational landscape for both primary and metastatic tumours. This may thus form a basis for personalized medical care and shed light on the molecular pathogenesis of metastatic lung adenocarcinoma
Cytochrome P450-mediated co-metabolism of fluoroquinolones by Haematococcus lacustris for simultaneously promoting astaxanthin and lipid accumulation
Microalgae-based antibiotic removal treatment has attracted attention because of its low carbon and sustainable
advantages. The microalgal co-metabolism system with a suitable carbon source leads to enhanced performance
of pollutant removal. However, currently, limited knowledge is available for the removal of fluoroquinolone
using a microalgae-mediated co-metabolism system. In this study, we first investigated that the biotic processes
by alga Haematococcus lacustris in the co-metabolism system by adding glycerol would be the main contributors
responsible for the removal of 10 mg/L ofloxacin (OFL) with the efficiency of 79.73% and the removal of 10 mg/
L enrofloxacin (ENR) with the efficiency of 54.10%, respectively. Furthermore, we found that pyruvate from
glycerol was converted into substrates and precursors, thereby resulting in the significant accumulations of
microalgal astaxanthin and lipid. The astaxanthin content of H. lacustris was achieved at 4.81% and 4.69%
treated with OFL and ENR in the presence of glycerol, with 16.04% and 14.55% of lipid content, respectively.
The proposed metabolites and pathways were identified to plausibly explain the biodegradation of fluoroquinolone
by H. lacustris. The molecular analyses demonstrated that cytochrome P450 (CYP450) enzymes are
responsible for the biodegradation of fluoroquinolone, and it was further verified that fluoroquinolones might
insert into CYP450 to finally form an efficient and tight binding conformation by molecular dynamic simulation.
These findings provide a microalgae-based route for feasible and sustainable biodegradation of antibiotics using
a co-metabolism strategy comprising glycerol as a carbon source, with the synergistic accumulation of valuable
products.peer-reviewe
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