Additional file 1: of Assessing the effectiveness of household-level focal mass drug administration and community-wide mass drug administration for reducing malaria parasite infection prevalence and incidence in Southern Province, Zambia: study protocol for a community randomized controlled trial

Web appendix containing supporting information. (DOCX 60 kb

Study participant flow.

Sixteen clusters were selected from all eligible health facility catchment areas in Southern Province, Zambia.</p

Population seroprevalence of a selection of long- and short-term malaria antigens.

(DOCX)</p

Day 1 individuals recruited for RCD follow-ups on days 30 and 90 by age and gender.

(DOCX)</p

Reference standard curves for <i>P</i>. <i>falciparum</i> 3D7 strain showing the relationship between parasitaemia and Ct / DNA concentration.

The assay was performed in duplicate (n = 3). (TIF)</p

Fig 4 -

Seropositivity for each of the health facility catchment populations to long-term antigens, stratified by age (left and middle), and short-term antigens (right). Health facilities are ordered in each panel according to maximum seropositivity observed. RFTAT control (orange) and RFDA intervention (blue) arms are shown accordingly. Bars show 95% confidence intervals.</p

List of antigens used in the serology multiplex bead assay with positivity observed and numbers.

(DOCX)</p

Number of individuals enrolled and treated in the CoRE study by arm.

Number of individuals enrolled and treated in the CoRE study by arm.</p

CONSORT checklist.

(DOC)</p

S1 Protocol -

(DOCX)</p