Surgical Instrument Sets for the Small Animal Practice

The use of standardized pre-sterilized instrument sets in small animal surgery contributes to practice efficiency by having sterilized instruments ready when they are needed. A basic set of instruments is prepared and can be supplemented with special packs for specific applications. A disadvantage to this approach is that the expense when the packs are originally established may be greater

Electrospray Ionization with High-Resolution Mass Spectrometry as a Tool for Lignomics: Lignin Mass Spectrum Deconvolution

Capability to characterize lignin, lignocellulose, and their degradation products is essential for development of new renewable feedstocks. Electrospray ionization high-resolution time-offlight mass spectrometry (ESI HR TOF MS) method was developed expanding the lignomics toolkit while targeting the simultaneous detection of low and high molecular weight (MW) lignin species. The effect of a broad range of electrolytes and various ionization conditions on ion formation and ionization effectiveness was studied using a suite of mono-, di- and triarene lignin model compounds as well as intact lignin. Contrary to the previous studies, the positive ionization mode was found to be more effective for methoxy-substituted arenes and polyphenols, i.e., species of a broadly varied MW structurally similar to the native lignin. For the first time, we report an effective formation of multiply charged species of lignin with the subsequent mass spectrum deconvolution in the presence of 100 mmol·L-1 formic acid in the positive ESI mode. The developed method enabled the detection of lignin species with an MW between 150 and 9,000 Da or higher, depending on the mass analyzer. The obtained Mn and Mw values of 1,500 and 2,500 Da, respectively, were in good agreement with those determined by gel permeation chromatography. Furthermore, the deconvoluted ESI mass spectrum was similar to that obtained with matrixassisted laser desorption/ionization (MALDI) TOF MS, yet featuring a higher signal-to-noise ratio. The formation of multiply charged species was confirmed with ESI ion mobility HR Q-TOF MS

Surgical Instrument Sets for the Small Animal Practice

The use of standardized pre-sterilized instrument sets in small animal surgery contributes to practice efficiency by having sterilized instruments ready when they are needed. A basic set of instruments is prepared and can be supplemented with special packs for specific applications. A disadvantage to this approach is that the expense when the packs are originally established may be greater.</p

Modeling nonlinear response from distributed damage and kissing bonds

A large number of models have been proposed in the field of nonlinear elastic wave propagation in damaged solids to validate experimental observations with theoretical predictions. In this contribution, we distinguish between "distributed" and "interface" implementations of nonlinearity, and deduce particular properties for the differing models. For the models of distributed damage, we prove that many of the currently suggested models can be seen as particular cases of the PM-model for specific protocols and parameters. In addition, we illustrate the potentiality of interface models to validate experimental results reported by other research groups

Efficient Extraction of 3D Bone Cells Descriptors from Micro-CT Images

International audienceWhile cell analysis is conventionally performed on 2D slices, novel micro and nano-CT system opens new perspectives in this area. Here, we show that synchrotron radiation (SR) micro-CT is well suited to analyze the 3D distribution of osteocyte lacunae in bone tissue. Osteocytes are receiving increasing interest in the comprehension of bone diseases. Here, we propose a fast automated method to extract 3D quantitative morphological descriptors on these cells. To this aim, after a fast connected component analysis applied on the segmented image, a moment-based approach and intrinsic volumes are calculated to derive 3D descriptors on each object. The segmentation is refined by eliminating artifacts according to some descriptors. Validation of segmentation and experimental results on twelve bone samples are presented. This method is efficient and is believed to open new perspectives to quantify physiopathologic changes at the cell level

3D bone cells quantification from 3D micro-CT images

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