8 research outputs found
DTU Synthetic Promoter Library Standard
The purpose of this RFC is to outline a method for generating a BioBrick compatible Synthetic Promoter Library (SPL) within bacteria in order to fine-tune the expression of BioBrick parts and devices
In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods
Evaluation of hMLH1-R389W Mutation in Patients with Suspected Hereditary Nonpolyposis Colorectal Cancer
The Communication of Science: A case study: The Communication of GMO in China and the UK
The influence of resveratrol treatment in combination with tsc1 knock out on the mammalian cell growth
The effect of light intensity on the growth of Myrophyllium Aquaticum and Myrophyllium Tuberculatum
Retracing Circulating Tumour Cells for Biomarker Characterization after Enumeration
Background
Retracing and biomarker characterization of individual circulating tumour cells (CTCs) may potentially contribute to personalized metastatic cancer therapy. This is relevant when a biopsy of the metastasis is complicated or impos‐ sible to acquire.
Methods
A novel disc format was used to map and retrace individual CTCs from breast-cancer patients and nucleated cells from healthy blood donors using the CytoTrack platform. For proof of the retracing concept, CTC HER2 characterization by immunofluorescence was tested.
Results
CTCs were detected and enumerated in three of four blood samples from breast-cancer patients and the locations of each individual CTCs were mapped on the discs. Nucleat‐ ed cells were retraced on seven discs with 96.6%±8.5% recovery on five fields of view on each disc. Shifting of field of view for retracing was measured to 4-29 μm. In a blood sample from a HER2-positive breast-cancer patient, CTC enumeration and mapping was followed by HER2 charac‐ terization and retracing to demonstrate downstream immunofluorescence analysis of the CTC.
Conclusion
Mapping and retracing of CTCs enables downstream analysis of individual CTCs for existing and future cancer genotypic and phenotypic biomarkers. Future studies will uncover this potential of the novel retracing technology