Chemical structure of <i>N</i>-methyl-substituted [^99mTc]BT2 ([^99mTc]MBT2).

<p>Chemical structure of <i>N</i>-methyl-substituted [^99mTc]BT2 ([^99mTc]MBT2).</p

In vitro autoradiograms of mouse brain sections labeled with ^99mTc-Ham complexes.

<p>The sections are from wild-type (A, E, and I) and Tg2576 (B, F, and J) mice. The same Tg2576 mouse brain sections are stained with thioflavin-S (C, G, and K). Blocking studies with PIB are also performed using the adjacent brain sections (D, H, and L). ^aData from our previous study (Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163969#pone.0163969.ref030" target="_blank">30</a>).</p

Synthetic route for MBTHam.

<p>Synthetic route for MBTHam.</p

In vitro autoradiograms of human brain sections from a patient with CAA.

<p>The sections are labeled with [^99mTc]MBT2 (A) and [^99mTc]BT2B (C). The adjacent brain section is immunostained with an antibody against β-amyloid (B). ^aData from our previous study (Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163969#pone.0163969.ref028" target="_blank">28</a>).</p

Radiochromatograms of [^99mTc]BT2 (A) and [^99mTc]MBT2 (B).

<p>Radiochromatograms of [^99mTc]BT2 (A) and [^99mTc]MBT2 (B).</p

Half-maximal inhibitory concentration (IC₅₀, μM) for the binding of PIB to Aβ aggregates determined using ^99mTc-Ham complexes as ligands.

<p>Half-maximal inhibitory concentration (IC₅₀, μM) for the binding of PIB to Aβ aggregates determined using ^99mTc-Ham complexes as ligands.</p

Percent radiochemical purity of [^99mTc]MBT2 and [^99mTc]BT2 as a function of time.

<p>Percent radiochemical purity of [^99mTc]MBT2 and [^99mTc]BT2 as a function of time.</p

Baseline characteristics of patients in three groups.

<p>Baseline characteristics of patients in three groups.</p

Multivariate linear regression analysis of aortic valvular fibrosis in patients with severe AS.

<p>Multivariate linear regression analysis of aortic valvular fibrosis in patients with severe AS.</p

Histological and immunohistochemical findings of aortic valve in TAV-AS, CBAV-AS, and CBAV-AR.

<p>A: Inflammation; Inflammation with CD3 is seen in each group. More inflammatory cell infiltration is noted in TAV-AS and CBAV-AS compared with CBAV-AR. Red arrows indicate T cells infiltrations in valves. Upper photomicrographs are hematoxylin-eosin (H&E) staining, and lower photomicrographs are immunohistochemical staining for CD3. B: Neovascularization; Neovascularizations in TAV-AS and CBAV-AS are more intensive than those in CBAV-AR. Upper photomicrographs are H&E staining, and lower photomicrographs are immunohistochemical staining for von Willebrand factor (vWF). Blue arrows indicate the expression of vWF. C: Calcium deposition; The degrees of calcium deposition are higher in TAV-AS and CBAV-AS compared with CBAV-AR. Upper photomicrographs are Masson’s trichrome (MT) staining, and lower photomicrographs are immunohistochemical staining for osteopontin (OPN). Red arrows indicate the expression of osteopontin. D: Cholesterol deposition; The degrees of cholesterol deposition are higher in TAV-AS and CBAV-AS compared with CBAV-AR. Photomicrographs are H&E staining. E: Fibrosis; Valvular fibrosis is more severely noted in CBAV-AS compared with TAV-AS and CBAV-AR. Photomicrographs are MT staining. F: Immunohistochemical staining for tenascin-C. Red arrows indicate the expression of tenascin-C. Tenascin-C deposit is more diffuse and intensive in CBAV-AS valve.</p