19 research outputs found
Rapid detection of trace bacteria in biofluids using porous monoliths in microchannels
We present advancements in microfluidic technology for rapid detection of as few as 10 rickettsial organisms in complex biological samples. An immuno-reactive filter, macroporous polyacrylamide monolith (PAM), fabricated within a microfluidic channel enhances solid-phase immuno-capture, staining and detection of targeted bacteria. Bacterial cells in samples flowing through the channel are forced to interact with the PAM filter surface due to size exclusion, overcoming common transport and kinetic limitations for rapid (min), high-efficiency (~100%) capture. In the process, targeted cells in sample volumes of 10 ?l to >100 ?l are concentrated within a sub-50 nl region at the PAM filter edge in the microchannel, thus concentrating them over 1000-fold. This significantly increases sensitivity, as the hydrophilic PAM also yields low non-specific immuno-fluorescence backgrounds with samples including serum, blood and non-targeted bacteria. The concentrated target cells are detected using fluorescently-labeled antibodies. With a single 2.0�0�3 mm PAM filter, as few as 10 rickettsial organisms per 100 祃 of lysed blood sample can be analyzed within 60 min, as compared to hours or even days needed for conventional detection methods. This method is highly relevant to rapid, multiplexed, low-cost point of care diagnostics at early stages of infection where diagnostics providing more immediate and actionable test results are needed to improve patient outcomes and mitigate potential natural and non-natural outbreaks or epidemics of rickettsial diseases
Spatiotemporal Mapping of Concentration Polarization Induced pH Changes at Nanoconstrictions
Under an applied electric field, concentration polarization (CP) arises from ion permselectivity of most nanoporous materials and biological ion channels. We present novel methods to quantitatively assess CP-induced spatiotemporal changes of pH that may significantly impact transport dynamics, device functionality, and physicochemical properties of molecular analytes in devices with nanofluidic constrictions. We measured pH fluctuations of >1.5 pH units and changes extending over 100's of micrometers from nanoconstrictions. The degree of change depends on key system parameters including buffer composition, surface charge, and strength of electric field. The results highlight the importance of neglected contributions of pH changes, and the approach can aid characterization and manipulation of mass transport in nanofluidic systems
Microfluidic Digital Isoelectric Fractionation for Rapid Multidimensional Glycoprotein Analysis
Here we present an integrated microfluidic device for
rapid and
automated isolation and quantification of glycoprotein biomarkers
directly from biological samples on a multidimensional analysis platform.
In the first dimension, digital isoelectric fractionation (dIEF) uses
discrete pH-specific membranes to separate proteins and their isoforms
into precise bins in a highly flexible spatial arrangement on-chip.
dIEF provides high sample preconcentration factors followed by immediate
high-fidelity transfer of fractions for downstream analysis. We successfully
fractionate isoforms of two potential glycoprotein cancer markers,
fetuin and prostate-specific antigen (PSA), with 10 min run time,
and results are compared qualitatively and quantitatively to conventional
slab gel IEF. In the second dimension, functionalized monolithic columns
are used to capture and detect targeted analytes from each fraction.
We demonstrate rapid two-dimensional fractionation, immunocapture,
and detection of C-reactive protein (CRP) spiked in human serum. This
rapid, flexible, and automated approach is well-suited for glycoprotein
biomarker research and verification studies and represents a practical
avenue for glycoprotein isoform-based diagnostic testing
Microfluidic Digital Isoelectric Fractionation for Rapid Multidimensional Glycoprotein Analysis
Here we present an integrated microfluidic device for
rapid and
automated isolation and quantification of glycoprotein biomarkers
directly from biological samples on a multidimensional analysis platform.
In the first dimension, digital isoelectric fractionation (dIEF) uses
discrete pH-specific membranes to separate proteins and their isoforms
into precise bins in a highly flexible spatial arrangement on-chip.
dIEF provides high sample preconcentration factors followed by immediate
high-fidelity transfer of fractions for downstream analysis. We successfully
fractionate isoforms of two potential glycoprotein cancer markers,
fetuin and prostate-specific antigen (PSA), with 10 min run time,
and results are compared qualitatively and quantitatively to conventional
slab gel IEF. In the second dimension, functionalized monolithic columns
are used to capture and detect targeted analytes from each fraction.
We demonstrate rapid two-dimensional fractionation, immunocapture,
and detection of C-reactive protein (CRP) spiked in human serum. This
rapid, flexible, and automated approach is well-suited for glycoprotein
biomarker research and verification studies and represents a practical
avenue for glycoprotein isoform-based diagnostic testing
Microfluidic Digital Isoelectric Fractionation for Rapid Multidimensional Glycoprotein Analysis
Here we present an integrated microfluidic device for
rapid and
automated isolation and quantification of glycoprotein biomarkers
directly from biological samples on a multidimensional analysis platform.
In the first dimension, digital isoelectric fractionation (dIEF) uses
discrete pH-specific membranes to separate proteins and their isoforms
into precise bins in a highly flexible spatial arrangement on-chip.
dIEF provides high sample preconcentration factors followed by immediate
high-fidelity transfer of fractions for downstream analysis. We successfully
fractionate isoforms of two potential glycoprotein cancer markers,
fetuin and prostate-specific antigen (PSA), with 10 min run time,
and results are compared qualitatively and quantitatively to conventional
slab gel IEF. In the second dimension, functionalized monolithic columns
are used to capture and detect targeted analytes from each fraction.
We demonstrate rapid two-dimensional fractionation, immunocapture,
and detection of C-reactive protein (CRP) spiked in human serum. This
rapid, flexible, and automated approach is well-suited for glycoprotein
biomarker research and verification studies and represents a practical
avenue for glycoprotein isoform-based diagnostic testing
Microfluidic Digital Isoelectric Fractionation for Rapid Multidimensional Glycoprotein Analysis
Here we present an integrated microfluidic device for
rapid and
automated isolation and quantification of glycoprotein biomarkers
directly from biological samples on a multidimensional analysis platform.
In the first dimension, digital isoelectric fractionation (dIEF) uses
discrete pH-specific membranes to separate proteins and their isoforms
into precise bins in a highly flexible spatial arrangement on-chip.
dIEF provides high sample preconcentration factors followed by immediate
high-fidelity transfer of fractions for downstream analysis. We successfully
fractionate isoforms of two potential glycoprotein cancer markers,
fetuin and prostate-specific antigen (PSA), with 10 min run time,
and results are compared qualitatively and quantitatively to conventional
slab gel IEF. In the second dimension, functionalized monolithic columns
are used to capture and detect targeted analytes from each fraction.
We demonstrate rapid two-dimensional fractionation, immunocapture,
and detection of C-reactive protein (CRP) spiked in human serum. This
rapid, flexible, and automated approach is well-suited for glycoprotein
biomarker research and verification studies and represents a practical
avenue for glycoprotein isoform-based diagnostic testing
A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish <i>In Vitro</i> Tissue Interfaces
<div><p>Microfluidic barrier tissue models have emerged as advanced <i>in vitro</i> tools to explore interactions with external stimuli such as drug candidates, pathogens, or toxins. However, the procedures required to establish and maintain these systems can be challenging to implement for end users, particularly those without significant in-house engineering expertise. Here we present a module-based approach that provides an easy-to-use workflow to establish, maintain, and analyze microscale tissue constructs. Our approach begins with a removable culture insert that is magnetically coupled, decoupled, and transferred between standalone, prefabricated microfluidic modules for simplified cell seeding, culture, and downstream analysis. The modular approach allows several options for perfusion including standard syringe pumps or integration with a self-contained gravity-fed module for simple cell maintenance. As proof of concept, we establish a culture of primary human microvascular endothelial cells (HMVEC) and report combined surface protein imaging and gene expression after controlled apical stimulation with the bacterial endotoxin lipopolysaccharide (LPS). We also demonstrate the feasibility of incorporating hydrated biomaterial interfaces into the microfluidic architecture by integrating an ultra-thin (< 1 <b>μ</b>m), self-assembled hyaluronic acid/peptide amphiphile culture membrane with brain-specific Young’s modulus (~ 1kPa). To highlight the importance of including biomimetic interfaces into microscale models we report multi-tiered readouts from primary rat cortical cells cultured on the self-assembled membrane and compare a panel of mRNA targets with primary brain tissue signatures. We anticipate that the modular approach and simplified operational workflows presented here will enable a wide range of research groups to incorporate microfluidic barrier tissue models into their work.</p></div
Differential height vs. predicted flow rate and shear stress.
<p>Differential height vs. predicted flow rate and shear stress.</p
A Reversibly Sealed, Easy Access, Modular (SEAM) Microfluidic Architecture to Establish <i>In Vitro</i> Tissue Interfaces - Fig 3
<p>a) Layers comprising the gravity fed perfusion module. b) Side view of connected perfusion and culture modules. The fluidic circuit (inlet reservoir, aa, through the culture module to the outlet reservoir, bb) was gently primed with medium using a syringe. c) Once filled, surface tension kept the medium from spilling out of the reservoirs, and the difference in hydrostatic pressure <b>Δ</b>P induced fluid flow from inlet to outlet reservoirs.</p
SEAM readouts after LPS stimulation.
<p>a) To explore SEAM readouts that mimic vascular inflammation, human microvasular endothelial cells were seeded and transferred to the coupled culture and micro-perfusion modules. b) After apical simulation with LPS for 4 hours, cells were and stained in-channel and the membrane was transferred to a glass slide to explore ICAM1 surface expression after exposure to stimulation. Scale bar = 40 μm. c) To demonstrate rapid and robust RNA isolation, inserts were removed from the architecture and transferred to off-chip RNA isolation. Statistically significant (p<0.001) differences in mRNA expression were observed in ICAM1, CXCL1, CXCL2, and CCL2 with VFW (endothelial marker) remaining constant. SEAM is able to replicate both gene expression and differential surface protein responses to LPS stimulation with an easy to use workflow.</p