Enteric coating of granules containing the probiotic Lactobacillus acidophilus

In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid medium and disintegration time of 1 h in buffer pH 6.8. The amount of Eudragit L30D-55 in the capsules correlated with gastric juice resistance. The best protective qualities against artificial gastric juice were observed when capsules were prepared from granules composed of L. acidophilus, corn starch, lactose monohydrate, polyvinylpyrrolidone and coated with 12.5 % (m/V) of Eudragit L30D-55. Capsule formulation of L. acidophilus in edible broth medium suspension serves as a cheap alternative to the expensive freeze-drying procedure for preparing L. acidophilus. In addition, the enteric coating using Eudragit L30D-55 could protect probiotics from the acidic gastric environment and enhance the bioactivity of probiotics along with replacement of pathogenic microbes in human intestine

Screening Of Agro-Wastes As Growth Substrate, Quantification And Antimicrobial Activity Of Bioactive Metabolites Of Lactobacillus Species

Tujuan kajian ini adalah untuk menskrin dan kenalpasti sisa pertanian yang sesuai dalam membangunkan media pertumbuhan yang selektif untuk Lactobacillus. Metabolit bioaktif yang dihasilkan oleh Lactobacillus dikira dan aktiviti antimikrob ditentukan. The objective of this study was to screen and identify a suitable agro-waste in the development of a selective growth medium for Lactobacillus

The Impact of Blood Cupping (Al-Hijama) on Serum Creatinine and Uric Acid Levels in Patients from Hadhramout, Yemen

Cupping is a wonderful healing tool that has been employed for hundreds of years by numerous traditional folklore practitioners. Cupping treats many diseases including eliminating blood pollutants like uric acid and creatinine. The aim of this study to evaluate the impact of cupping on blood pressure, uric acid concentration, and creatinine levels. Sixty (60) random patient between the ages of (20 -70) years were selected, the blood samples were collected before cupping and after cupping for the evaluation. The result showed that the levels of creatinine and uric acid decreased significantly (p < 0.05) after cupping for both creatinine and uric acid. Moreover, the study showed that blood pressure decreased significantly (p < 0.05) after cupping. The result concluded that cupping has positive impact on health by modulating creatinine, uric acid blood levels and decreasing blood pressure

Enteric coating of granules containing the probiotic Lactobacillus acidophilus

In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid medium and disintegration time of 1 h in buffer pH 6.8. The amount of Eudragit L30D-55 in the capsules correlated with gastric juice resistance. The best protective qualities against artificial gastric juice were observed when capsules were prepared from granules composed of L. acidophilus, corn starch, lactose monohydrate, polyvinylpyrrolidone and coated with 12.5 % (m/V) of Eudragit L30D-55. Capsule formulation of L. acidophilus in edible broth medium suspension serves as a cheap alternative to the expensive freeze-drying procedure for preparing L. acidophilus. In addition, the enteric coating using Eudragit L30D-55 could protect probiotics from the acidic gastric environment and enhance the bioactivity of probiotics along with replacement of pathogenic microbes in human intestin

Treatment of Water Contaminated with Polycyclic Aromatic Hydrocarbons (PAHs): A Review of Various Techniques, Constraints, and Field Procedures

Clean water is vital in the creation of energy and sustenance of life. However, the pollution of water and the absence of potable water are global problems resulting from agricultural and industrial activities. We have witnessed significant growth in the pollution of water by organic compounds like PAH. Experts have made an effort to establish favorable techniques for the treatment of PAH polluted water. These techniques are either thermal, biological, physical or chemical. Bioremediation, chemical oxidation, solid-phase extraction, coagulation, photocatalytic degradation and adsorption using graphenes, mesoporous silica and agricultural wastes are techniques that are already in use in the field treatment of PAHs while electrokinetic remediation and nanoremediation are still in their developmental stage. Several reviews on the treatment of sediments and soils contaminated with PAHs have been published, but only a few reviews center mainly on the removal of PAHs in water. Therefore, this review aims to provide information on the techniques used in the treatment of water contaminated with PAHs. Techniques that are already in use and those that are in their developmental stage were reviewed. The successes of these methods, limitations, constraints and field procedures were analyzed and this will help to inform decision making