SD-OCT images of WT and Retinitis Pigmentosa (RP) rabbit retinas and histology of the visual streak in an RP rabbit.

<p>(A) A fundus infrared image of a WT rabbit retina, including optic nerve head (ONH) and visual streak. The area between dotted lines is the visual streak. (B) A vertical SD-OCT image along the green arrow in panel A, which passes through the center of the ONH. On this vertical image, the scleral ring was regarded as the lower margin of the ONH. (C) A magnified OCT image of the area enclosed by the blue square in panel B, which includes the visual streak. (D) A fundus infrared image of a RP rabbit retina, including the ONH and visual streak. (E) A vertical SD-OCT image of a 20-week-old RP rabbit along the green arrow in panel D. (F) A magnified OCT image of the area enclosed by the blue square in panel E. The 2.2 mm width of this OCT section was vertically cut between 1.8 mm and 4.0 mm ventral to the inferior edge of the ONH. A dotted arrow indicates the region of the visual streak. (G) Hematoxylin-Eosin staining of a retinal section corresponding to the area in the OCT image in F. Scale Bar = 200 µm (B, E), 100 µm (C, F), and 50 µm (G). RNFL, retinal nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; GCC, ganglion cell complex; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; ELM, external limiting membrane; IS, inner segments of photoreceptors; OS, outer segments of photoreceptors; IS/OS, junctions between IS and OS; RPE, retinal pigment epithelium; and BM, Bruch's membrane.</p

Ultrastructure of photoreceptors in WT and RP rabbits.

<p>(A, B) Ultrastructure of photoreceptors in 4-week-old WT rabbits. The inner (IS) and outer segments (OS) of the photoreceptors were regular and dense. There are no vesicles in the extracellular spaces (*). (C–E) Ultrastructure of the photoreceptors in the 4-week-old RP rabbits. The IS and OS were less organized than those in the WT rabbits. In the magnified image (D), the RP rabbit retina showing many small vesicles (arrowheads) accumulated in the extracellular spaces (indicated with * in panel C). The vesicles appeared to be cleaved from the IS into the extracellular space around the photoreceptors (arrows in panel E). (F) Ultrastructural immunohistochemistry by using an anti-rhodopsin antibody. The small vesicles (disintermediated arrowheads) in the extracellular spaces around the photoreceptors exhibit black dots indicating the presence of rhodopsin.</p

Time-dependent changes in morphological features of the retina and visual function in the RP rabbits.

<p>(A) SD-OCT images beneath the visual streak in an RP rabbit at 4, 6, 10, and 20 weeks and in a 20-week-old WT rabbit. The total retinal and ONL thickness in the RP rabbits decreased with age. The IS and OS were highly reflective in the RP rabbits compared with the WT rabbits. ONL, outer nuclear layer; and OS, outer segments of photoreceptors. (B) Hematoxylin-eosin staining of retinas in 4-, 6-, 10-, and 20-week-old RP and 20-week-old WT rabbits. The ONL in RP rabbits thinned with age. In 20-week-old RP rabbits, only 1–2 layers of nuclei were detected in the ONL. (C) Representative scotopic electroretinograms of 4-, 6-, 10-, and 20-week-old RP and 20-week-old WT rabbits. (D) The a-wave amplitude of the mixed rod and cone response. The amplitude was smaller in the RP rabbits than in the WT rabbits. The differences between the WT and RP rabbits were significant at all study points between 4 and 20 weeks. *<i>P</i><0.05, ***<i>P</i><0.001 (unpaired <i>t</i>-test). Scale Bar = 100 µm in A, and 50 µm in B. ONL, outer nuclear layer; ELM, external limiting membrane; IS/OS, junctions between inner segment (IS) and outer segment (OS); RPE, retinal pigment epithelium; and BM, Bruch's membrane.</p