生活体験学習大分フォーラム「変貌する幼少年期の生活体験をどう捉えるか」

日時 : 2009年１月24日(土) 13：40- 会場 : 大分大学教育福祉科学部棟及び一般教育棟 登壇者 : 三田井万(社会福祉法人ポプラ会旦野原保育所) 櫻井晴美(鴛野校区児童育成クラブ) 村上和子(社会福祉法人シンフォニー) 矢野修(大分県教育庁生涯学習課) 藤澤敏夫(大分市立稙田東中学校) コーディネーター : 山岸治男(大分大学

Complete Polar Lipid Composition of Thermoplasma acidophilum HO-62 Determined by High-Performance Liquid Chromatography with Evaporative Light-Scattering Detection

Polar ether lipids of Thermoplasma acidophilum HO-62 were purified by high-performance liquid chromatography with an evaporative light-scattering detector. Structures of purified lipids were investigated by capillary gas chromatography, mass spectrometry, and nuclear magnetic resonance. Three types of ether lipids were found: phospholipids, glycolipids, and phosphoglycolipids. The two phospholipids had glycerophosphate as the phosphoester moiety. The seven glycolipids had different combinations of gulose, mannose, and glucose, which formed mono- or oligosaccharides. The eight phosphoglycolipids with two polar head groups contained glycerophosphate as the phosphoester moiety and gulose alone or gulose and mannose, which formed mono- or oligosaccharides, as the sugar moiety. Although gulose is an unusual sugar in nature, several glyco- and phosphoglycolipids contained gulose as one of the sugar moieties in Thermoplasma acidophilum. All the ether lipids had isopranoid chains of C(40) or C(20) with zero to three cyclopentane rings. The structures of these lipids including four new glycolipids and three new phosphoglycolipids were determined, and a glycosylation process for biosynthesis of these glycolipids was suggested