14 research outputs found
Thinking Outside the Box Turtle: Public Perceptions of an Imperiled Species
Eastern box turtles (Terrapene carolina carolina) experience negative impacts from human activities. Collection for the pet trade or mortalities caused by lawn mowers and vehicles are detrimental to populations, which have experienced rapid decline in Indiana. Understanding perceptions and attitudes held about species can help outreach. This study aims to observe how perceptions and fear response differ between a) genders, b) contact with box turtles, and c) conservation group membership. Mail surveys were administered to 1,378 residents of the Blue River Watershed in Southern Indiana. Respondents rated box turtles on 11 semantic differential pairs and reported their agreement towards a series of Likert-scale questions that measured conservation and fear related attitudes. While fear response was relatively low across groups, women had had a significantly higher fear response (1.71 out of 5) than men (1.53). Women had higher agreement that box turtles are important to the Blue River ecosystem (4.08 vs. 3.77). Respondents who had encountered a box turtle associated box turtles with positive phrases and had higher agreement to ecosystem importance (3.94 vs. 3.39). Members of conservation groups had significantly higher agreement to ecosystem importance (4.25) and approval of government spending on box turtle conservation (3.31) compared to non-members (3.81 and 2.94). There was no correlation between level of fear response and recognition of ecosystem importance. Exposure to box turtles can be a mechanism of instilling positive perceptions of the species. Outreach may not need to address “fearful” perceptions of this species for individuals to still value box turtle conservation
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Population Structure of Geosmithia morbida, the Causal Agent of Thousand Cankers Disease of Walnut Trees in the United States
The ascomycete Geosmithia morbida and the walnut twig beetle Pityophthorus juglandis are associated with thousand
cankers disease of Juglans (walnut) and Pterocarya (wingnut). The disease was first reported in the western United States
(USA) on several Juglans species, but has been found more recently in the eastern USA in the native range of the highly
susceptible Juglans nigra. We performed a comprehensive population genetic study of 209 G. morbida isolates collected
from Juglans and Pterocarya from 17 geographic regions distributed across 12 U.S. states. The study was based on sequence
typing of 27 single nucleotide polymorphisms from three genomic regions and genotyping with ten microsatellite primer
pairs. Using multilocus sequence-typing data, 197 G. morbida isolates were placed into one of 57 haplotypes. In some
instances, multiple haplotypes were recovered from isolates collected on the same tree. Twenty-four of the haplotypes
(42%) were recovered from more than one isolate; the two most frequently occurring haplotypes (H02 and H03)
represented 36% of all isolates. These two haplotypes were abundant in California, but were not recovered from Arizona or
New Mexico. G. morbida population structure was best explained by four genetically distinct groups that clustered into
three geographic regions. Most of the haplotypes isolated from the native range of J. major (Arizona and New Mexico) were
found in those states only or present in distinct genetic clusters. There was no evidence of sexual reproduction or genetic
recombination in any population. The scattered distribution of the genetic clusters indicated that G. morbida was likely
disseminated to different regions at several times and from several sources. The large number of haplotypes observed and
the genetic complexity of G. morbida indicate that it evolved in association with at least one Juglans spp. and the walnut
twig beetle long before the first reports of the disease
The Role of Wildlife Value Orientations in Framing Interactions with Wildlife Near the Home: A Mixed-methods Analysis of Self-reported Problems with Wildlife
Population Structure of <i>Geosmithia morbida</i>, the Causal Agent of Thousand Cankers Disease of Walnut Trees in the United States
<div><p>The ascomycete <i>Geosmithia morbida</i> and the walnut twig beetle <i>Pityophthorus juglandis</i> are associated with thousand cankers disease of <i>Juglans</i> (walnut) and <i>Pterocarya</i> (wingnut). The disease was first reported in the western United States (USA) on several <i>Juglans</i> species, but has been found more recently in the eastern USA in the native range of the highly susceptible <i>Juglans nigra</i>. We performed a comprehensive population genetic study of 209 <i>G. morbida</i> isolates collected from <i>Juglans</i> and <i>Pterocarya</i> from 17 geographic regions distributed across 12 U.S. states. The study was based on sequence typing of 27 single nucleotide polymorphisms from three genomic regions and genotyping with ten microsatellite primer pairs. Using multilocus sequence-typing data, 197 <i>G. morbida</i> isolates were placed into one of 57 haplotypes. In some instances, multiple haplotypes were recovered from isolates collected on the same tree. Twenty-four of the haplotypes (42%) were recovered from more than one isolate; the two most frequently occurring haplotypes (H02 and H03) represented 36% of all isolates. These two haplotypes were abundant in California, but were not recovered from Arizona or New Mexico. <i>G. morbida</i> population structure was best explained by four genetically distinct groups that clustered into three geographic regions. Most of the haplotypes isolated from the native range of <i>J. major</i> (Arizona and New Mexico) were found in those states only or present in distinct genetic clusters. There was no evidence of sexual reproduction or genetic recombination in any population. The scattered distribution of the genetic clusters indicated that <i>G. morbida</i> was likely disseminated to different regions at several times and from several sources. The large number of haplotypes observed and the genetic complexity of <i>G. morbida</i> indicate that it evolved in association with at least one <i>Juglans</i> spp. and the walnut twig beetle long before the first reports of the disease.</p></div
Coordinates of 57 (A) and 55 (B) haplotypes of <i>Geosmithia morbida</i> from the MLST-DAPC model.
<p>The most distant cluster (cluster 4 in green) comprised of haplotypes H32 and H33 is identified <b>(A)</b>, as well as the coordinates of all remaining haplotypes when H32 and H33 were excluded <b>(B)</b>. A comparison between the assignments of the MLST-DAPC and MLST-STRUCTURE models are shown in detail. Pie charts give the probability of assignment of haplotypes to the four genetic clusters obtained in the four-clusters-MLST-STRUCTURE model. They are represented by colors, cluster 1 = blue, cluster 2 = red, cluster 3 = yellow and cluster 4 = green. Haplotypes in the box (in <b>B</b>) were amplified for better resolution.</p
Locations, hosts, haplotypes and genetic clusters based on the four-cluster-MLST-DAPC model, for <i>Geosmithia morbida</i> isolates.
<p>* Geographical regions are depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112847#pone-0112847-g001" target="_blank">Fig. 1B</a>.</p><p>** Isolates selected in the first trial of MLST analysis.</p>☆<p>Isolates deposited at Centraalbureau voor Schimmelcultures as CBS 124663 (1217) and CBS 124664 (1218).</p><p>Isolates underlined were subjected to SSR analysis.</p>A-P<p>Common letters indicate isolates from different cankers of the same tree.</p><p>Collectors are as follows: R. M. Bostock (RMB), T. W. Coleman (TWC), W. Cranshaw (WC), P.L. Dallara (PLD), G. Durham (GD), E. Fichtner (EF), S. Fraedrich (SF), A. D. Graves (ADG), K.J. Greby (KJG), B. Hammon (BH), J.E. Henrich (JEH), S.M. Hishinuma (SMH), D. Leatherman (DL), C. Leslie (CL), A. Liu (AL), J. McKenna (JM), L.M. Ohara (LMO), J. Pscheidt (JP), M. Putnam (MP), S. Schlarbaum (SS), S. J. Seybold (SJS), N. Tisserat (NT), C. Utley (CU), D.L. Wood (DLW). All isolations were made in the laboratory of NT with the exception of isolate 1513, which was made in the laboratory of RMB.</p><p>Locations, hosts, haplotypes and genetic clusters based on the four-cluster-MLST-DAPC model, for <i>Geosmithia morbida</i> isolates.</p
Primers tested on MLST analysis of <i>Geosmithia morbida</i> isolates.
<p>* BT Geo was designed based on <i>G. morbida</i> genome, inwardly oriented after amplification by using BT22 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112847#pone.0112847-ODonnell1" target="_blank">[36]</a>.</p><p>Primers tested on MLST analysis of <i>Geosmithia morbida</i> isolates.</p
Pairwise <i>F</i><sub>ST</sub> values calculated for the three-population geographical model observed with Hudson’s Permtest.
<p>Significant (<i>P</i><0.05) values are denoted by (*).</p><p>Geographical regions: 1) NM_AZ/Ce CA/No CA/No CO/TN; 2) Ce AZ; and 3) Sw CA/OR_WA/So CO.</p><p>Pairwise <i>F</i><sub>ST</sub> values calculated for the three-population geographical model observed with Hudson’s Permtest.</p
Coordinates of the SSR profile of 112 isolates of <i>G. morbida</i> based on DAPC analysis: of a theoretical <i>K</i> = 6 (A); and the same, but excluding the distant isolates in green, which resulted in <i>K</i> = 3 (B).
<p>Coordinates of the SSR profile of 112 isolates of <i>G. morbida</i> based on DAPC analysis: of a theoretical <i>K</i> = 6 (A); and the same, but excluding the distant isolates in green, which resulted in <i>K</i> = 3 (B).</p
Unrooted phylogenetic tree of <i>Geosmithia</i> species based on ITS/BT sequences.
<p>A Bayesian analysis was performed for 1,500,000 generations by using a GTR-gamma distributed model of evolution (invariant sites). Bayesian percentages (≥50%) are depicted above each branch, and maximum likelihood bootstrap values (≥500) obtained by using PhyML (default parameters) are shown below most branches. <i>Geosmithia morbida</i> haplotypes are color coded according to their genetic cluster assignment (four-cluster-MLST-DAPC model, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0112847#pone-0112847-g003" target="_blank">Figure 3</a>) and haplotypes sharing the same ITS and BT sequences are co-located. Leaves pertaining to the same branch were arranged together according to their cluster assignment. GenBank accession numbers of other <i>Geosmithia</i> spp. are identified within parenthesis.</p