Supranutritional supplementation of vitamin E influences mitochondrial proteome profile of post-mortem longissimus lumborum from feedlot heifers

Supplementation of vitamin E improves beef colour stability by minimizing lipid oxidation-induced myoglobin oxidation. Mitochondria affect myoglobin redox stability, and dietary vitamin E influences mitochondrial functionality in skeletal muscles. Nonetheless, the influence of vitamin E on the mitochondrial proteome of beef skeletal muscles has yet to be  investigated. Therefore, the objective of this study was to examine the effect of dietary vitamin E on mitochondrial proteome of post-mortem beef longissimus lumborum (LL) muscle. Beef LL muscle samples (24 hours post-mortem) were obtained from the carcasses of nine (n = 9) vitamin E-fed (VITE) (1000 IU vitamin E for 89 days) and nine (n = 9) control (CONT) (diet without supplemental vitamin E) heifers. The mitochondrial proteome was analysed using two-dimensional gel electrophoresis and mass spectrometry, and nine differentially abundant spots were identified. All the differentially abundant spots were over-abundant in CONT, and the proteins were electron transport chain enzymes (NADH dehydrogenase iron-sulphur protein 8, NADH dehydrogenase flavoprotein 2, and cytochrome c oxidase subunit 5B), metabolic enzymes (succinate-CoA ligase (ADP-forming) subunit beta; dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex; pyruvate dehydrogenase protein X component), and enzymes involved in ATP regeneration (creatine kinase S-type). The low abundance of these proteins in VITE may decrease mitochondrial activity, resulting in low oxidative activity. These findings suggest that the strong antioxidant activity of vitamin E leads to less expression of mitochondrial oxidative enzymes in beef skeletal muscles.Keywords: Beef colour, lipid oxidation, mitochondrial enzyme

An insight into high-temperature deformation mechanism of magnesium in-situ composite through development of Johnson-Cook and constitutive model

The establishment of deformation mechanisms of Mg-metal matrix composite (Mg-MMC) is important to improve the high-temperature challenging applications. In this present work, an AZ91/TiC + TiB2 hybrid in-situ Mg-MMC was synthesized, and its deformation mechanisms were studied through a uniaxial hot compressive test at different temperatures and strain rates. The Johnson-Cook (JC) model and constitutive equation were established using experimental stress-strain data. Through the development of JC model, it was revealed that the TiC–TiB2 particles enhanced the yield strength parameter and increased the activation energy of the in-situ composite compared to the parent alloy. The load-shifting capability and grain refinement were found to be the dominating mechanisms, which effectively restricted dislocation movement during deformation, resulting in improved deformation resilience of the composite. A detailed study of JC model and constitutive equation parameters was analyzed with a focus on their microstructures

Hydroclimatological perspective of the Kerala flood of 2018

Flood is among the deadliest disasters in India, and the frequency of floods and extreme precipitation events is projected to increase under the warming climate. The frequency of floods in India varies geographically as some regions are more prone to floods than the others. The Kerala flood of 2018 caused enormous economic damage, affected millions of people, and resulted in the death of more than 400 people. Here we provide a hydroclimatological perspective on the Kerala flood of 2018. Using the observations and model simulations from the Variable Infiltration Capacity (VIC) model, we show that the 2018 extreme precipitation and runoff conditions that caused flooding were unprecedented in the record of the past 66 years (1951–2017). Our results show that mean monsoon precipitation has significantly declined while air temperature has significantly increased during 1951–2017 in Kerala. The drying and warming trends during the monsoon season resulted in a declined total runoff in large part of the state in the last 66 years. Apart from the mean hydroclimatic conditions, extreme precipitation, and extreme total runoff have also declined from 1951 to 2017. However, 1 and 2-day extreme precipitation and extreme runoff conditions in August 2018 exceeded substantially from the long-term 95th percentiles recorded during 1951–2017. Since there is no increase in mean and extreme precipitation in Kerala over the last six decades, the extreme event during August 2018 is likely to be driven by anomalous atmospheric conditions due to climate variability rather anthropogenic climate warming. The severity of the Kerala flood of 2018 and the damage caused might be affected by several factors including land use/land cover change, antecedent hydrologic conditions, reservoir storage and operations, encroachment of flood plains, and other natural factors. The impacts of key drivers (anthropogenic and natural) on flood severity need to be established to improve our understanding of floods and associated damage.by Vimal Mishra and Harsh L. Sha