DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: Comparison with CAS 200

BACKGROUND: For solid tumors, image cytometry has been shown to be more sensitive for diagnosing DNA content abnormalities (aneuploidy) than flow cytometry. Image cytometry has often been performed using the semi-automated CAS 200 system. Recently, an Automated Cellular Imaging System (ACIS) was introduced to determine DNA content (DNA index), but it has not been validated. METHODS: Using the CAS 200 system and ACIS, we compared the DNA index (DI) obtained from the same archived formalin-fixed and paraffin embedded tissue samples from Barrett's esophagus related lesions, including samples with specialized intestinal metaplasia without dysplasia, low-grade dysplasia, high-grade dysplasia and adenocarcinoma. RESULTS: Although there was a very good correlation between the DI values determined by ACIS and CAS 200, the former was 25% more sensitive in detecting aneuploidy. ACIS yielded a mean DI value 18% higher than that obtained by CAS 200 (p < 0.001; paired t test). In addition, the average time required to perform a DNA ploidy analysis was shorter with the ACIS (30–40 min) than with the CAS 200 (40–70 min). Results obtained by ACIS gave excellent inter-and intra-observer variability (coefficient of correlation >0.9 for both, p < 0.0001). CONCLUSION: Compared with the CAS 200, the ACIS is a more sensitive and less time consuming technique for determining DNA ploidy. Results obtained by ACIS are also highly reproducible

On embeddings of Morrey type spaces between weighted Lebesgue or Stummel spaces with application to Herz spaces

We study embeddings of Morrey type spaces M-p,M-q,M-omega(R-n), 1 1. In the case q > p we show that the embedding, in general, cannot hold with omega = w. For global Morrey type spaces we also prove embeddings into Stummel spaces. Similar embeddings for complementary Morrey type spaces are obtained. We also study inverse embeddings of weighted Lebesgue spaces L-p(R-n, w) into Morrey type and complementary Morrey type spaces. Finally, using our previous results on relations between Herz and Morrey type spaces, we obtain "for free" similar embeddings for Herz spaces.Start-up Grant of United Arab Emirates University, Al Ain, United Arab Emirates [G00002994]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-01-00223]; TUBITAK and Russian Foundation for Basic researchTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [20-51-46003