IL-17 and synaptopodin protein expression of an anti-thy1 glomerulonephritic animal on d5.

<p>Representative images showing glomerular IL-17 and synaptopodin protein expression in renal tissue sections of an anti-thy1 glomerulonephritic animal on d5 after disease induction, in paraffin-embedded immunostained sections at x400 magnification. IL-17 green, synaptopodin red, nucleus blue, merge yellow.</p

IL-17 and PECAM-1 protein expression of an anti-thy1 glomerulonephritic animal on d5.

<p>Representative images showing glomerular IL-17 and PECAM-1 protein expression in renal tissue sections of an anti-thy1 glomerulonephritic animal on d5 after disease induction, in paraffin-embedded immunostained sections at x400 magnification. IL-17 green, PCAM-1 red, nucleus blue, merge yellow.</p

Glomerular protein expression and <i>in situ</i> hybridization of IL-17 mRNA of anti-thy1 glomerulonephritic animals on d5.

<p>Representative images showing glomerular protein expression (A) and <i>in situ</i> hybridization (B) of IL-17 mRNA (dark purple) of anti-thy1 glomerulonephritic animals on d5 after disease induction, in paraffin-embedded renal sections at x400 magnification. The black arrows indicate IL-17protein or mRNA deposition.</p

Immunostained tissue sections over the time course of acute anti-thy1 glomerulonephritis (aGN).

<p>Representative pictures showing IL-17-immunostained kidneys from con (PBS-injected control) animals, d5 = animals on d5 after aGN induction, d10 = animals on day 10 after aGN induction, d15 = animals on day 15 after aGN induction and nc = negative control are shown. IL-17 red, nucleus blue (magnification x 400).</p

Glomerular mRNA expression of IL-6, TGF-β1 and IL-17 over the time course of acute anti-thy1 glomerulonephritis (aGN).

<p>The disease was induced by a single OX-7 antibody injection. Control animals (con) received the same volume of PBS. Animals were sacrificed, glomeruli were harvested and graded using the sieving technique and mRNA was isolated using TRIZOL reagent. After reverse transcription mRNA, expression levels were measured by real time PCR. For analysis, the relative quantification ΔΔCP method was used. (# p<0.05 vs. con).</p

Immunofluorescence of NRK-52E after stimulation.

<p>Representative images showing IL-17 (red) and TGF-β1 (green) expression, merge (yellow), nucleus (blue) of NRK-52E cells. Row one shows untreated NRK-52E cells, NRK-52E cells stimulated by 25 mM glucose (high glucose), 5 ng/ml TGF-β1, 25 ng/ml IL-17, 5 ng/ml TGF-β1+ 10 ng/ml IL-6. Row two depicts TGF-β1 and IL-17 expression in NRK-52E after treatment by 10 μM TGF-β receptor blocker SB431542 after stimulation by 25 mM glucose (high glucose), 5 ng/ml TGF-β1, 25 ng/ml IL-17, 5ng/ml TGF-β1+ 10 ng/ml IL-6. All cells are starved 24 h for cell cycle synchronization and stimulated for 48 h at 37°C under 5% CO₂ and then immune-stained (magnification x1000).</p

Proteinuria over the time course of acute anti-thy1 glomerulonephritis.

<p>The disease was induced by a single OX-7 antibody injection. Control animals received the same volume of PBS. Animals were housed in metabolic cages, urine was collected for 24 h and proteinuria was measured by a modified pyrogallol red method 24h = animals after 24 hours after aGN induction, d5 = animals on day 5 after aGN induction, d10 = animals on day 10 after aGN induction, d15 = animals on day 15 after aGN induction, d20 = animals on day 20 after aGN induction are shown. (# p< 0.001 vs. con; *p<0.05 vs. d5).</p

TGF-β1, IL-6 and IL-17 mRNA expression of NRK-52E after glucose stimulation.

<p>Relative TGF-β1 (A), IL-6 (B) and IL-17 (C) mRNA expression of non-stimulated NRK-52E (nc) and at different time points after stimulation by 25 mM glucose or 20 mM mannose as an osmotic control. Analysis utilized real-time PCR and was normalized to β-actin as housekeeping gene. (# p <0.05 vs. nc; * p<0.001 vs. nc).</p

TGF-β1, IL-6 and IL-17 mRNA expression of NRK-52E after co-stimulation by TGF-β1 and IL-6.

<p>Relative TGF-β1 (A), IL-6 (B) and IL-17 (C) mRNA expression of non-stimulated NRK-52E (nc) and at different time points after co-stimulation by 5 ng/ml TGF- β1 and 10 ng/ml IL-6. Analysis utilized real-time PCR and was normalized to β-actin as housekeeping gene. (# p <0.05 vs. nc; * p<0.001 vs. nc).</p

TGF-β1, IL-6 and IL-17 mRNA expression of NRK-52E after glucose, IL-6, TGF-β1 or IL-17 stimulation.

<p>TGF-β1, IL-6 and IL-17 mRNA expression in NRK-52E cells 15 min after stimulation by 25 mM glucose, 10 ng/ml IL-6, 5 ng/ml TGF-β1 or 25 ng/ml IL-17. Analysis utilized real-time PCR and was normalized to β-actin as housekeeping gene. (# p <0.05 vs. nc; * p<0.01 vs. nc).</p