Uso de tecnologias em lavouras de trigo tecnicamente assistidas no Paraná - safra 2008.

bitstream/CNPT-2010/40770/1/p-do111.pd

The Genome Sequence DataBase: towards an integrated functional genomics resource

During 1998 the primary focus of the Genome Sequence DataBase (GSDB; http://www.ncgr.org/gsdb ) located at the National Center for Genome Resources (NCGR) has been to improve data quality, improve data collections, and provide new methods and tools to access and analyze data. Data quality has been improved by extensive curation of certain data fields necessary for maintaining data collections and for using certain tools. Data quality has also been increased by improvements to the suite of programs that import data from the International Nucleotide Sequence Database Collaboration (IC). The Sequence Tag Alignment and Consensus Knowledgebase (STACK), a database of human expressed gene sequences developed by the South African National Bioinformatics Institute (SANBI), became available within the last year, allowing public access to this valuable resource of expressed sequences. Data access was improved by the addition of the Sequence Viewer, a platform-independent graphical viewer for GSDB sequence data. This tool has also been integrated with other searching and data retrieval tools. A BLAST homology search service was also made available, allowing researchers to search all of the data, including the unique data, that are available from GSDB. These improvements are designed to make GSDB more accessible to users, extend the rich searching capability already present in GSDB, and to facilitate the transition to an integrated system containing many different types of biological data

Uso de tecnologias em lavouras de trigo tecnicamente assistidas no Paraná - safra 2014.

bitstream/item/147667/1/ID43753-2016DO160.pd

Uso de tecnologias em lavouras de trigo tecnicamente assistidas no Paraná - safra 2012.

Registra e analisa informações sobre a triticultura paranaense correspondente à safra de 2012, assim como efetua diagnóstico acerca dos desafios para o avanço da cultura, no contexto dos sistemas de produção regionais.bitstream/item/103757/1/2014-documentos-online148.pd

Monitoramento de Phakopsora pachyrhizi na safra 2018/2019 para tomada de decisão do controle da ferrugem-asiática da soja.

bitstream/item/201231/1/CIRCULAR-TECNICA-150-OL.pd

Rapid sequencing of DNA based on single molecule detection

Sequencing the human genome is a major undertaking considering the large number of nucleotides present in the genome and the slow methods currently available to perform the task. We have recently reported on a scheme to sequence DNA rapidly using a non-gel based technique. The concept is based upon the incorporation of fluorescently labeled nucleotides into a strand of DNA, isolation and manipulation of a labeled DNA fragment and the detection of single nucleotides using ultra-sensitive laser-induced fluorescence detection following their cleavage from the fragment. Detection of individual fluorophores in the liquid phase was accomplished with time-gated detection following pulsed-laser excitation. The photon bursts from individual rhodamine 6G (R6G) molecules travelling through a laser beam have been observed as have bursts from single fluorescently modified nucleotides. Using two different biotinylated nucleotides as a model system for fluorescently labeled nucleotides, we have observed synthesis of the complementary copy of M13 bacteriophage. Work with fluorescently labeled nucleotides is underway. We have observed and manipulated individual molecules of DNA attached to a microbead with an epifluorescence microscope. 16 refs., 3 figs., 1 tab