Agarose gel (1%) analysis of PCR product of SXT integrase from IDH isolates and their transconjugants.

<p>PCR products obtained using genomic DNA templates from clinical isolates or their transconjugants have been electrophoresed in different lanes as follows: Lane M : 1 kb ladder (Fermentas); Lane 1: Positive control <i>V.cholerae</i> O139 MO10; Lane 2: Recipient <i>E. coli</i> XL-1 Blue; Lanes 3 and 4: Negative controls of no DNA template and SXT-negative IDH02095 isolate respectively; Lanes 5 and 6 : IDH01572 (SXT-positive) isolate and its transconjugant respectively; Lanes 7 and 8 : IDH01738 (SXT-positive) isolate and its transconjugant respectively.</p

Antibiotic susceptibility profile of 119 clinical isolates from Kolkata, India, in 2009.

<p>AMP, Ampicillin; CHL, Chloramphenicol; CIP, Ciprofloxacin; COT, Co-Trimoxazole; GEN, Gentamicin; KAN, Kanamycin; NAL, Nalidixic Acid; NEO, Neomycin; NOR, Norfloxacin; PB, Polymixin B; STR, Streptomycin; SUL, Sulfisoxazole; TET, Tetracycline; TRI, Trimethoprim.</p

Antimicrobial susceptibility of IDH01572, IDH01738 and their transconjugants.

<p>The antibiotic names are as described in legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056477#pone-0056477-g001" target="_blank">figure 1</a>.</p><p>Bold face indicates the resistance traits from recipient XL-1Blue cells.</p