Reduction of radiation dose by application of optimized filtered backprojection algorithm

X-Ray tomography is one of widespread methods of medical diagnostics. Due to its opportunity to visualize inside structure of scanned object it allows to diagnose, for example, cancer cells. Modern medical tomographic setups have lots of advantages such as high resolution, which allows to visualize objects of very small size, high level of reproducibility, which allows to verify obtained results, simplicity of utilization etc. There is certain amount of improvements still can be applied. One of most significant challenges is reducing of radiation dose. Diagnostics of complex structures sometimes requires more time of measurements. That means that dose will increase with time. This work proposes optimized filtered backprojection algorithm as solution for the problem of radiation dose level

First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1)

The antigenic structures of the haemotrophic Mycoplasma suis, an epicellular parasite of porcine erythrocytes, are largely unknown due to its unculturability. In this study, serological proteome and mass spectrometry analyses allowed the characterization of M. suis proteins targeted by the porcine antibody response: two proteins with characteristics of heat shock proteins, two proteins with characteristics of glycolytic enzymes, a RNA helicase- and an actin-like protein. The DnaK-like protein of M. suis (HspA1) was further analysed genetically and functionally. Its encoding gene (M. suis a1 gene) is 1.830 bp in size and corresponds to a 67 kDa protein. Immunoelectron microscopy verified the surface accessibility of HspA1 in M. suis. Recombinant HspA1 expressed in Escherichia coli demonstrated ATPase activity and antigenicity in experimentally infected pigs. In conclusion, this first identification and recombinant expression of an antigenic protein of M. suis provides the basis for the development of vaccines and new in vitro diagnostic assays