Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis

The development of granulomas is a major histopathological feature of tuberculosis. Very little information is available concerning the physiology and functions of different cell types in the tuberculous granulomas. The aim of this study was to compare the epithelioid cells (ECs) and multinucleated giant cells (MGCs) in the granulomas caused by Mycobacterium tuberculosis complex organisms. Lymph node biopsies from 30 cases of lymphadenitis were studied for expression of the secreted mycobacterial protein MPT64, caspase 3 as a marker of apoptosis, apoptosis-related proteins (Fas Ligand, Fas and Bax) and inflammatory cytokines (interleukin-10, transforming growth factor-β (TGF-β), tumour necrosis factor-α and interferon-γ) by immunohistochemistry. MGCs more often contained M. tuberculosis secretory antigen MPT64 (p < 0.001) and expressed more TGF-β (p = 0.004) than ECs. The total number of apoptotic MGCs was higher than the number of apoptotic ECs (p = 0.04). Interestingly, there was a significant negative correlation between apoptosis and MPT64 expression in MGCs (r = −0.569, p = 0.003), but not in ECs, implying that the heavy antigen load would lead to inhibition of apoptosis in these cells. When compared with ECs, higher percentage of MGCs expressed Fas Ligand and Fas (p < 0.004). The role of MGCs may thus be different from surrounding ECs and these cells by virtue of higher mycobacterial antigen load, more TGF-β and reduced apoptosis may contribute towards persistence of infection

What Can We Learn from Urban Crisis?

The irreversible transition towards urban living entails complex challenges and vulnerabilities for citizens, civic authorities, and the management of global commons. Many cities remain beset by political, infrastructural, social, or economic fragility, with crisis arguably becoming an increasingly present condition of urban life. While acknowledging the intense vulnerabilities that cities can face, this article contends that innovative, flexible, and often ground-breaking policies, practices, and activities designed to manage and overcome fragility can emerge in cities beset by crisis. We argue that a deeper understanding of such practices and the knowledge emerging from contexts of urban crisis may offer important insights to support urban resilience and sustainable development. We outline a simple conceptual representation of the interrelationships between urban crisis and knowledge production, situate this in the context of literature on resilience, sustainability, and crisis, and present illustrative examples of real-world practices. In discussing these perspectives, we reflect on how we may better value, use, and exchange knowledge and practice in order to address current and future urban challenges

What Can We Learn from Urban Crisis?

The irreversible transition towards urban living entails complex challenges and vulnerabilities for citizens, civic authorities, and the management of global commons. Many cities remain beset by political, infrastructural, social, or economic fragility, with crisis arguably becoming an increasingly present condition of urban life. While acknowledging the intense vulnerabilities that cities can face, this article contends that innovative, flexible, and often ground-breaking policies, practices, and activities designed to manage and overcome fragility can emerge in cities beset by crisis. We argue that a deeper understanding of such practices and the knowledge emerging from contexts of urban crisis may offer important insights to support urban resilience and sustainable development. We outline a simple conceptual representation of the interrelationships between urban crisis and knowledge production, situate this in the context of literature on resilience, sustainability, and crisis, and present illustrative examples of real-world practices. In discussing these perspectives, we reflect on how we may better value, use, and exchange knowledge and practice in order to address current and future urban challenges

What Can We Learn from Urban Crisis?

The irreversible transition towards urban living entails complex challenges and vulnerabilities for citizens, civic authorities, and the management of global commons. Many cities remain beset by political, infrastructural, social, or economic fragility, with crisis arguably becoming an increasingly present condition of urban life. While acknowledging the intense vulnerabilities that cities can face, this article contends that innovative, flexible, and often ground-breaking policies, practices, and activities designed to manage and overcome fragility can emerge in cities beset by crisis. We argue that a deeper understanding of such practices and the knowledge emerging from contexts of urban crisis may offer important insights to support urban resilience and sustainable development. We outline a simple conceptual representation of the interrelationships between urban crisis and knowledge production, situate this in the context of literature on resilience, sustainability, and crisis, and present illustrative examples of real-world practices. In discussing these perspectives, we reflect on how we may better value, use, and exchange knowledge and practice in order to address current and future urban challenges.publishedVersio

What Can We Learn from Urban Crisis?

The irreversible transition towards urban living entails complex challenges and vulnerabilities for citizens, civic authorities, and the management of global commons. Many cities remain beset by political, infrastructural, social, or economic fragility, with crisis arguably becoming an increasingly present condition of urban life. While acknowledging the intense vulnerabilities that cities can face, this article contends that innovative, flexible, and often ground-breaking policies, practices, and activities designed to manage and overcome fragility can emerge in cities beset by crisis. We argue that a deeper understanding of such practices and the knowledge emerging from contexts of urban crisis may offer important insights to support urban resilience and sustainable development. We outline a simple conceptual representation of the interrelationships between urban crisis and knowledge production, situate this in the context of literature on resilience, sustainability, and crisis, and present illustrative examples of real-world practices. In discussing these perspectives, we reflect on how we may better value, use, and exchange knowledge and practice in order to address current and future urban challenges

Norske floker - urbane utfordringer - Rapport fra forskningsprosjektet Learning Flexibility 2018

Et stort demografisk skifte til urbane levesett og den økende urbaniseringen gir både muligheter og utfordringer. Blant de største utfordringene kan vi nevne ressursknapphet klimaendringer og byvekst, sammen med mer komplekse og sammensatte samfunn. Selv i høyt utviklede og urbaniserte land som Norge er det viktig å ta tak i framtidens utfordringer i dag for å sikre en bærekraftig byutvikling og levelige byer for senere generasjoner. Denne rapporten peker på noen av hovedutfordringene for norske byer. Disse vil trolig vil kreve økt oppmerksomhet nå og i de kommende årene. Gjennom samtaler og diskusjoner med berørte parter belyser rapporten spørsmål knyttet til byutvikling, energi, styresett og deltakelse i norske byer de neste tre tiårene, samt hvordan man kan angripe disse. Å utforske fleksibilitet som utgangspunkt for å takle hurtig og uforutsigbar endring har stått sentralt i samtalene. Ved å diskutere hvordan fleksibilitet kan arte seg på forskjellige områder som byutvikling, energi, styresett og medvirkning, kan vi trekke opp forbindelser mellom disse områdene