Modification of polyelectrolyte multilayer coatings using nanoparticles to optimize adhesion and proliferation of different cell types

Adapting characteristics of biomaterials specifically for in vitro and in vivo applications is becoming increasingly important in order to control interactions between material and biological systems. These complex interactions are influenced by surface properties like chemical composition, charge, mechanical and topographic attributes. In many cases it is not useful or even not possible to alter the base material but changing surface, to improve biocompatibility or to make surfaces bioactive, may be achieved by thin coatings. An already established method is the coating with polyelectrolyte multilayers (PEM). To adjust adhesion, proliferation and improve vitality of certain cell types, we modified the roughness of PEM coatings. We included different types nanoparticles (NP’s) in different concentrations into PEM coatings for controlling surface roughness. Surface properties were characterized and the reaction of 3 different cell types on these coatings was tested

Additional file 3: Figure S1. of SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Melting curve analysis demonstrating the non-specific amplification by primer combinations F + R3, F + R4 and F3 + R4 in the presence of mosquito-derived RNA and specificity of F + R3 in a panel of flavivirus and alphavirus RNA. a. Melting peak analysis for the F + R4 primer pair b. Melting peak analysis for the F3 + R4 primer pair c. Melting peak analysis for the F + R3 primer pair. d. Melting peak analysis for the F + R3 primer pairs within a panel of flavivirus and alphavirus RNA. Abbreviation: NTC, Negative control. (TIFF 2225 kb

Additional file 4: Figure S2. of SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Melting peak analysis for the rRT-PCR assay performance in field-caught ZIKV-infected mosquitoes. The evaluation is based on the primer combination F2 + R3. The melting peak for ZIKV infected mosquitoes (1–7) and ZIKV non-infected mosquito species (8–14) falls within the same range as the positive control (ATCC® VR-84). (TIFF 679 kb

Additional file 2: of Construction sites as an important driver of dengue transmission: implications for disease control

Table S1. Mosquito breeding habitats and larvae counts detected in construction sites and residential premises from 2013 to 2016 (DOCX 12 kb

Additional file 3 Table S3. of Microbial survey of ready-to-eat salad ingredients sold at retail reveals the occurrence and the persistence of Listeria monocytogenes Sequence Types 2 and 87 in pre-packed smoked salmon

Primers used for the detection of Listeria spp. and L. monocytogenes, as well as the characterisation of E. coli, S. aureus, B. cereus and L. monocytogenes (DOCX 28 kb

Additional file 1 Table S1. of Microbial survey of ready-to-eat salad ingredients sold at retail reveals the occurrence and the persistence of Listeria monocytogenes Sequence Types 2 and 87 in pre-packed smoked salmon

Descriptions of ingredients in pre-packed salads. Table S2. Breakdown of salad bar ingredients collected. (DOCX 17 kb