6 research outputs found
General Method for Generating Circular Gradients of Active Proteins on Nanofiber Scaffolds Sought for Wound Closure and Related Applications
Scaffolds
functionalized with circular gradients of active proteins
are attractive for tissue regeneration because of their enhanced capability
to accelerate cell migration and/or promote neurite extension in a
radial fashion. Here, we report a general method for generating circular
gradients of active proteins on scaffolds composed of radially aligned
nanofibers. In a typical process, the scaffold, with its central portion
raised using a copper wire to take a cone shape, was placed in a container
(upright or up-side-down), followed by dropwise addition of bovine
serum albumin (BSA) solution into the container. As such, a circular
gradient of BSA was generated along each nanofiber. The bare regions
uncovered by BSA were then filled with an active protein of interest.
In demonstrating their potential applications, we used different model
systems to examine the effects of two types of protein gradients.
While the gradient of laminin and epidermal growth factor accelerated
the migration of fibroblasts and keratinocytes, respectively, from
the periphery toward the center of the scaffold, the gradient of
nerve growth factor promoted the radial extension of neurites from
the embryonic chick dorsal root ganglion. This method for generating
circular gradients of active proteins can be readily extended to different
types of scaffolds to suit wound closure and related applications
that involve cell migration and/or neurite extension in a radial fashion
PVDF Nanofiber Modified with ZnO Nanowires/Polydopamine for the Treatment of Sewage Containing Heavy Metals, Organic Dyes, and Bacteria
In various countries worldwide, the
issue of wastewater contamination
poses a significant threat due to its intricate composition of heavy
metals, organic dyes, and microorganisms, thereby complicating the
purification process. Consequently, researchers have expressed considerable
interest in materials capable of eliminating organic, heavy metal,
and microbial pollutants. This study focuses on the fabrication of
a water purification membrane (PDA/ZnO-NWs/PVDF) with a hierarchical
structure and the ability to remove multiple pollutants. The membrane
was created by modifying poly(vinylidene fluoride) (PVDF) nanofiber
with zinc oxide nanowires (ZnO-NWs) and reinforcing it with polydopamine
(PDA). The experimental results demonstrate that the PDA/ZnO-NWs/PVDF
membrane exhibits a range of functionalities, including long-lasting
superhydrophilicity, Cu(II) adsorption, photocatalytic degradation,
and antibacterial ability. The manipulation of the DA synthesis procedure
allows for the adjustment of the wettability, adsorption, and photocatalytic
and antibacterial activities of the PDA/ZnO-NWs/PVDF composite. According
to the Langmuir isotherm, the maximum Cu(II) adsorption capacity of
the PDA/ZnO-NWs/PVDF membrane is determined to be 65.75 mg/g, which
is significantly higher (27.26 mg/g) than that of the ZnO-NWs/PVDF
membrane (38.49 mg/g). The PDA/ZnO-NWs/PVDF composite exhibited a
notable degradation capacity toward rhodamine B under natural sunlight,
reaching a maximum of 5.97 mg/g. Additionally, the degradation rate
achieved during daylight hours was as high as 90.42%. Furthermore,
the antibacterial efficacy of the PDA/ZnO-NWs/PVDF composite against
both Gram-positive and Gram-negative bacteria approached 100%. This
work presents a promising approach for the treatment of wastewater
containing various coexisting contaminants
PVDF Nanofiber Modified with ZnO Nanowires/Polydopamine for the Treatment of Sewage Containing Heavy Metals, Organic Dyes, and Bacteria
In various countries worldwide, the
issue of wastewater contamination
poses a significant threat due to its intricate composition of heavy
metals, organic dyes, and microorganisms, thereby complicating the
purification process. Consequently, researchers have expressed considerable
interest in materials capable of eliminating organic, heavy metal,
and microbial pollutants. This study focuses on the fabrication of
a water purification membrane (PDA/ZnO-NWs/PVDF) with a hierarchical
structure and the ability to remove multiple pollutants. The membrane
was created by modifying poly(vinylidene fluoride) (PVDF) nanofiber
with zinc oxide nanowires (ZnO-NWs) and reinforcing it with polydopamine
(PDA). The experimental results demonstrate that the PDA/ZnO-NWs/PVDF
membrane exhibits a range of functionalities, including long-lasting
superhydrophilicity, Cu(II) adsorption, photocatalytic degradation,
and antibacterial ability. The manipulation of the DA synthesis procedure
allows for the adjustment of the wettability, adsorption, and photocatalytic
and antibacterial activities of the PDA/ZnO-NWs/PVDF composite. According
to the Langmuir isotherm, the maximum Cu(II) adsorption capacity of
the PDA/ZnO-NWs/PVDF membrane is determined to be 65.75 mg/g, which
is significantly higher (27.26 mg/g) than that of the ZnO-NWs/PVDF
membrane (38.49 mg/g). The PDA/ZnO-NWs/PVDF composite exhibited a
notable degradation capacity toward rhodamine B under natural sunlight,
reaching a maximum of 5.97 mg/g. Additionally, the degradation rate
achieved during daylight hours was as high as 90.42%. Furthermore,
the antibacterial efficacy of the PDA/ZnO-NWs/PVDF composite against
both Gram-positive and Gram-negative bacteria approached 100%. This
work presents a promising approach for the treatment of wastewater
containing various coexisting contaminants
PVDF Nanofiber Modified with ZnO Nanowires/Polydopamine for the Treatment of Sewage Containing Heavy Metals, Organic Dyes, and Bacteria
In various countries worldwide, the
issue of wastewater contamination
poses a significant threat due to its intricate composition of heavy
metals, organic dyes, and microorganisms, thereby complicating the
purification process. Consequently, researchers have expressed considerable
interest in materials capable of eliminating organic, heavy metal,
and microbial pollutants. This study focuses on the fabrication of
a water purification membrane (PDA/ZnO-NWs/PVDF) with a hierarchical
structure and the ability to remove multiple pollutants. The membrane
was created by modifying poly(vinylidene fluoride) (PVDF) nanofiber
with zinc oxide nanowires (ZnO-NWs) and reinforcing it with polydopamine
(PDA). The experimental results demonstrate that the PDA/ZnO-NWs/PVDF
membrane exhibits a range of functionalities, including long-lasting
superhydrophilicity, Cu(II) adsorption, photocatalytic degradation,
and antibacterial ability. The manipulation of the DA synthesis procedure
allows for the adjustment of the wettability, adsorption, and photocatalytic
and antibacterial activities of the PDA/ZnO-NWs/PVDF composite. According
to the Langmuir isotherm, the maximum Cu(II) adsorption capacity of
the PDA/ZnO-NWs/PVDF membrane is determined to be 65.75 mg/g, which
is significantly higher (27.26 mg/g) than that of the ZnO-NWs/PVDF
membrane (38.49 mg/g). The PDA/ZnO-NWs/PVDF composite exhibited a
notable degradation capacity toward rhodamine B under natural sunlight,
reaching a maximum of 5.97 mg/g. Additionally, the degradation rate
achieved during daylight hours was as high as 90.42%. Furthermore,
the antibacterial efficacy of the PDA/ZnO-NWs/PVDF composite against
both Gram-positive and Gram-negative bacteria approached 100%. This
work presents a promising approach for the treatment of wastewater
containing various coexisting contaminants
PVDF Nanofiber Modified with ZnO Nanowires/Polydopamine for the Treatment of Sewage Containing Heavy Metals, Organic Dyes, and Bacteria
In various countries worldwide, the
issue of wastewater contamination
poses a significant threat due to its intricate composition of heavy
metals, organic dyes, and microorganisms, thereby complicating the
purification process. Consequently, researchers have expressed considerable
interest in materials capable of eliminating organic, heavy metal,
and microbial pollutants. This study focuses on the fabrication of
a water purification membrane (PDA/ZnO-NWs/PVDF) with a hierarchical
structure and the ability to remove multiple pollutants. The membrane
was created by modifying poly(vinylidene fluoride) (PVDF) nanofiber
with zinc oxide nanowires (ZnO-NWs) and reinforcing it with polydopamine
(PDA). The experimental results demonstrate that the PDA/ZnO-NWs/PVDF
membrane exhibits a range of functionalities, including long-lasting
superhydrophilicity, Cu(II) adsorption, photocatalytic degradation,
and antibacterial ability. The manipulation of the DA synthesis procedure
allows for the adjustment of the wettability, adsorption, and photocatalytic
and antibacterial activities of the PDA/ZnO-NWs/PVDF composite. According
to the Langmuir isotherm, the maximum Cu(II) adsorption capacity of
the PDA/ZnO-NWs/PVDF membrane is determined to be 65.75 mg/g, which
is significantly higher (27.26 mg/g) than that of the ZnO-NWs/PVDF
membrane (38.49 mg/g). The PDA/ZnO-NWs/PVDF composite exhibited a
notable degradation capacity toward rhodamine B under natural sunlight,
reaching a maximum of 5.97 mg/g. Additionally, the degradation rate
achieved during daylight hours was as high as 90.42%. Furthermore,
the antibacterial efficacy of the PDA/ZnO-NWs/PVDF composite against
both Gram-positive and Gram-negative bacteria approached 100%. This
work presents a promising approach for the treatment of wastewater
containing various coexisting contaminants
Additional file 1 of Remote evaluation of sleep to enhance understanding of early dementia due to Alzheimer’s Disease (RESTED-AD): an observational cohort study protocol
Additional file 1: SM 1. Further Statistical Analysis of Data Pertaining to Secondary Hypotheses. SM 2. RESTED Sleep Questionnaire