1 research outputs found
Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure
Matrix
metalloproteinase 2 (MMP-2) has been considered a promising
molecular biomarker for cancer diagnosis due to its related dysregulation.
In this work, a core–satellite structure-powered ratiometric
surface-enhanced Raman scattering (SERS) nanosensor with high sensitivity
and specificity to MMP-2 was developed. The SERS nanosensor was composed
of a magnetic bead encapsulated within a 5,5′-dithiobis(2-nitrobenzoic
acid) (DTNB)-labeled gold shell as the capture core and a 4-mercaptobenzonitrile
(MBN)-encoded silver nanoparticle as the signal satellite, which were
connected through a peptide substrate of MMP-2. MMP-2-triggered cleavage
of peptides from the core surface resulted in a decrease of the SERS
intensity of MBN. Since the SERS intensity of DTNB was used as an
internal standard, the reliable and sensitive quantification of MMP-2
activity would be realized by the ratiometric SERS signal, with a
limit of detection as low as 2.067 ng/mL and a dynamic range from
5 to 100 ng/mL. Importantly, the nanosensor enabled a precise determination
of MMP-2 activity in tumor cell secretions, which may provide an avenue
for early diagnosis and classification of malignant tumors