Phytochemical analysis of major secondary metabolites present in methanolic extract of <i>Eichhornia crassipes</i>.

<p>Phytochemical analysis of major secondary metabolites present in methanolic extract of <i>Eichhornia crassipes</i>.</p

Diameter of inhibition zones (mm) around paper disc loaded with crude methanolic extract and fractions of <i>Eichhornia crassipes</i> against tested microalgae after 72–168h.

<p>Abbreviation: Dictyo.  =  <i>Dictyochloropsis splendid</i>; Nos.  =  <i>Nostoc piscinale</i>; Chl.  =  <i>Chlorella vulgaris</i>; Spir.  =  <i>Spirulina platensis</i>.</p><p>*Each value is presented as mean of triplicate treatments, LSD: Least different significantly at p≤0.01 according to Duncan's multiple range test. Data are expressed as mean ± S.D.</p

Fragmentation pattern of active ingredient (chemical structure and M. Wt) from <i>Eichhornia crassipes</i>.

<p>Fragmentation pattern of active ingredient (chemical structure and M. Wt) from <i>Eichhornia crassipes</i>.</p

Diameter of inhibition zones (mm) of crude methanolic extract and fractions of <i>Eichhornia crassipes</i> against tested microorganisms (bacteria after 24h and fungi after 72h).

<p>Abbreviation: Ab, antibiotic; Tetra, Tetracycline (antibacterial agent); Amph, Amphotericin B (antifungal agent).</p><p>*–: not tested; Each value is presented as mean of triplicate treatments, LSD: Least different significantly at p≤0.01 according to Duncan's multiple range test. Data are expressed as mean ± S.D.</p

Figure 5

<p>The mode of action of the Salicin and Saligenin to destroy immature White Blood Cells (WBC's) in leukemia patients.</p

GC/MS of crude methanolic extract of <i>Eichhornia crassipes</i>.

<p>*Rt: Retention time.</p

Predicted chemical structure of different active compounds separated from <i>Eichhornia crassipes</i>.

<p>Predicted chemical structure of different active compounds separated from <i>Eichhornia crassipes</i>.</p

Minimum inhibition concentration (MIC) of active fraction (A) from <i>Eichhornia crassipes</i>.

<p>*Each value is presented as mean of triplicate treatments, LSD: Least different significantly at p≤0.01 according to Duncan's multiple range test. Data are expressed as mean ± S.D.</p

Figure 1

<p>The effect of willow young leaves extracts on the viability of ALL cells. 7 ALL patients (acute lymphoblastic leukemia) 0.1 ml of the cell suspension containing 10⁵ cells was added to 5 tubes. To three of the tubes, 0.1 ml of the willow extract was added, while the other two tubes served as negative and positive controls. Culture medium was used instead of the willow extract for the negative control and the willow extract was added to the cells from healthy volunteers as a positive control. The tubes were incubated for 24 h and tested for their viability using the trypan blue.</p

Figure 4

<p>Effect of salix water extract on surviving of transplanted animals (EACC). One group fed on normal diet (negative control), second group was fed on normal diet and transplanted to the intraperitoneal (i.p) cavity with EACC at 2×10⁶ cells (0.2 ml) (positive control), third group fed the normal diet and EACC infected then each mouse daily forced to ingest orally via stomach tube about 0.2 ml of an aqueous willow extract (10% w/v), fourth group were treated daily like the third group except with 0.6 ml willow extract.</p