High damage potential of seed-borne spot blotch in organically grown spring barley in Denmark

Spot blotch of barley (Bipolaris sorokiniana, perfect state: Cochliobolus sativus) occurs wherever barley is grown but is normally not considered a major problem in Danish barley production. It is therefore not included in routine disease surveys or official variety testing in Denmark. However, recommended seed contamination thresholds are 30% for spring barley and 15% for winter barley. Several spring barley varieties were grown under organic (no seed dressing, mechanical weeding) and conventional (seed dressing with fungicides, herbicide application) production conditions at three sites in Denmark in 2003. The harvested seeds were analysed for contamination levels of seed-borne B. sorokiniana using a blotter method. The percentage of seeds contaminated with B. sorokiniana ranged from about 5 to 95 % and was highly depending on the site, production system and variety. Contamination levels of organically cultivated plots were about twice as high as those of plots receiving conventional treatments. The germinating ability of seeds was highly affected by the level of B. sorokiniana contamination and declined drastically at contamination levels above 60%. This effect appeared to differ among varieties. The 1000-grain weight was not affected by the level of spot blotch contamination of seeds. The results indicate that the importance of spot blotch may be underrated, especially in organic barley production and that the role of varietal resistance should be investigated. More results are expected from ongoing seed analyses

Resistente bygsorter begrænser problemer med bladplet i økologisk såsæd

Seed born net blotch is a threat to the organic barley seed production in Denmark because it often exceeds the legal 15% infestation threshold in organically produced barley seed lots. Relationships among initial seed infestation levels, disease development during the growing season, yield parameters and net blotch infestation of the harvested grains were examined. Highly significant positive correlations among initial seed infestation, primary infection of seedlings and disease severity during the growing season as well as among disease severity at flowering and infestation of the harvested grains were found. The seed infestation level was much less than proportionally related to the primary infection of seedlings and to the disease severity later in the season. All these variables were negatively correlated with single grain weight, fraction of large grains and total yield. Varietal resistance appeared to be a key determinant for disease severity and seed infestation. The results will be used to help develop more flexible net blotch infestation thresholds for organically produced barley seeds

Baseline anti-NS4a antibodies in combination with on-treatment quantitative HCV-RNA reliably identifies nonresponders to pegylated interferon-ribavirin combination therapy after 4 weeks of treatment

Background Early detection of nonresponders to hepatitis C therapy limits unnecessary exposure to treatment and its side-effects. A recent algorithm combining baseline anti-NS4a antibodies and on-treatment quantitative PCR identified nonresponders to a combination of interferon and ribavirin after 1 week of treatment. Aim To validate a stopping rule based on baseline anti-NS4a antibody levels and early on-treatment virological response in treatment-naive genotype 1 chronic hepatitis C patients treated with the current standard pegylated interferon and ribavirin combination therapy. Methods Eighty-nine genotype 1 patients from the Dynamically Individualized Treatment of hepatitis C Infection and Correlates of Viral/Host dynamics Study treated for 48 weeks with standard 180 mu g pegylated interferon (PEG-IFN)-alpha-2a (weekly) and ribavirin 1000-1200mg (daily) were analysed. Baseline anti-NS4a antibody enzyme-linked immunosorbent assay (NS4a AA 1687-1718) was performed on pretreatment serum. Hepatitis C virus-RNA was assessed at days 0, 1, 4, 7, 8, 15, 22, 29, weeks 6, 7, 8, 10, 12 and 6 weekly thereafter until end of treatment. Multiple regression logistic analysis was performed. Results Overall 54 of 89 (61%) patients achieved sustained virological response. A baseline anti-NS4a antibody titre less than 1/1250 correlated with absence of favourable initial viral decline according to variable response types (P=0.015). The optimal algorithm was developed using the combination of the absence of anti-NS4a Ab (= 100.000 IU/ml at week 4. This algorithm has a specificity of 43% and negative predictive value of 100% to detect nonresponse to standard PEG-IFN-alpha-2a and ribavirin therapy at fourth week of therapy (intention-to-treat analysis). Conclusion The decision to stop the therapy in genotype 1 chronic hepatitis C patients treated with PEG-IFN-alpha-2a and ribavirin can be confidently made after 4 weeks of treatment based on the absence of baseline anti-NS4a Ab and a week-4 hepatitis C virus-RNA above 100.000 IU/ml. Eur J Gastroenterol Hepatol 22:1443-1448 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins

Continuous Damage Fiber Bundle Model for Strongly Disordered Materials

We present an extension of the continuous damage fiber bundle model to describe the gradual degradation of highly heterogeneous materials under an increasing external load. Breaking of a fiber in the model is preceded by a sequence of partial failure events occurring at random threshold values. In order to capture the subsequent propagation and arrest of cracks, furthermore, the disorder of the number of degradation steps of material constituents, the failure thresholds of single fibers are sorted into ascending order and their total number is a Poissonian distributed random variable over the fibers. Analytical and numerical calculations showed that the failure process of the system is governed by extreme value statistics, which has a substantial effect on the macroscopic constitutive behaviour and on the microscopic bursting activity as well.Comment: 10 pages, 13 figure

A diagrammatic formulation of the kinetic theory of fluctuations in equilibrium classical fluids. VI. Binary collision approximations for the memory function for self correlation functions

We use computer simulation results for a dense Lennard-Jones fluid for a range of temperatures to test the accuracy of various binary collision approximations for the memory function for density fluctuations in liquids. The approximations tested include the moderate density approximation of the generalized Boltzmann-Enskog memory function (MGBE) of Mazenko and Yip, the binary collision approximation (BCA) and the short time approximation (STA) of Ranganathan and Andersen, and various other approximations derived by us using diagrammatic methods. The tests are of twotypes. The first is a comparison of the correlation functions predicted by each approximate memory function with the simulation results, especially for the self longitudinal current correlation function (SLCC). The second is a direct comparison of each approximate memory function with a memory function numerically extracted from the correlation function data. The MGBE memory function is accurate at short times but decays to zero too slowly and gives a poor description of the correlation function at intermediate times. The BCA is exact at zero time, but it predicts a correlation function that diverges at long times. The STA gives a reasonable description of the SLCC but does not predict the correct temperature dependence of the negative dip in the function that is associated with caging at low temperatures. None of the other binary collision approximations is a systematic improvement upon the STA. The extracted memory functions have a rapidly decaying short time part, much like the STA, and a much smaller, more slowly decaying part of the type predicted by mode coupling theory. Theories that use mode coupling commonly include a binary collision term in the memory function but do not discuss in detail the nature of that term. ...Comment: 18 pages, 10 figure